Calcium in PDB 1h71: Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
Enzymatic activity of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
All present enzymatic activity of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18':
3.4.24.40;
Protein crystallography data
The structure of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18', PDB code: 1h71
was solved by
V.Villeret,
F.Van Petegem,
N.Aghajari,
J.-P.Chessa,
C.Gerday,
R.Haser,
J.Van Beeumen,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
15.00 /
2.10
|
|
Space group
|
P 21 21 21
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
54.000,
57.550,
161.810,
90.00,
90.00,
90.00
|
|
R / Rfree (%)
|
16.4 /
22.1
|
Other elements in 1h71:
The structure of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' also contains other interesting chemical elements:
Calcium Binding Sites:
The binding sites of Calcium atom in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
(pdb code 1h71). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 8 binding sites of Calcium where determined in the
Psychrophilic Protease From Pseudoalteromonas 'Tac II 18', PDB code: 1h71:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
Calcium binding site 1 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 1 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca500
b:39.1
occ:1.00
|
O
|
P:VAL55
|
2.5
|
33.7
|
1.0
|
|
OD2
|
P:ASP114
|
2.5
|
36.8
|
1.0
|
|
OD2
|
P:ASP49
|
2.6
|
37.9
|
1.0
|
|
OD1
|
P:ASP53
|
2.6
|
39.0
|
1.0
|
|
ND2
|
P:ASN57
|
2.9
|
46.9
|
1.0
|
|
OD1
|
P:ASN57
|
3.0
|
47.5
|
1.0
|
|
ND2
|
P:ASN51
|
3.1
|
55.9
|
1.0
|
|
OD1
|
P:ASP114
|
3.2
|
32.6
|
1.0
|
|
CG
|
P:ASP114
|
3.2
|
36.9
|
1.0
|
|
CG
|
P:ASN57
|
3.3
|
42.4
|
1.0
|
|
CG
|
P:ASP53
|
3.3
|
43.8
|
1.0
|
|
CG
|
P:ASP49
|
3.6
|
38.6
|
1.0
|
|
OD2
|
P:ASP53
|
3.6
|
44.3
|
1.0
|
|
C
|
P:VAL55
|
3.7
|
37.9
|
1.0
|
|
CG
|
P:ASN51
|
3.8
|
57.0
|
1.0
|
|
N
|
P:ASN57
|
3.9
|
31.8
|
1.0
|
|
OD1
|
P:ASN51
|
4.1
|
59.8
|
1.0
|
|
CA
|
P:ASP49
|
4.2
|
37.9
|
1.0
|
|
OD1
|
P:ASP49
|
4.2
|
40.9
|
1.0
|
|
N
|
P:VAL55
|
4.2
|
39.8
|
1.0
|
|
N
|
P:ASP53
|
4.3
|
48.5
|
1.0
|
|
CA
|
P:ILE56
|
4.3
|
34.7
|
1.0
|
|
CB
|
P:ASP49
|
4.4
|
37.0
|
1.0
|
|
N
|
P:ASN51
|
4.5
|
49.1
|
1.0
|
|
C
|
P:ASP49
|
4.5
|
41.2
|
1.0
|
|
N
|
P:ILE56
|
4.5
|
34.6
|
1.0
|
|
CA
|
P:VAL55
|
4.5
|
39.6
|
1.0
|
|
C
|
P:ILE56
|
4.6
|
34.4
|
1.0
|
|
CB
|
P:ASN57
|
4.6
|
37.5
|
1.0
|
|
CB
|
P:ASP53
|
4.6
|
43.7
|
1.0
|
|
CG1
|
P:VAL55
|
4.6
|
45.1
|
1.0
|
|
CB
|
P:ASP114
|
4.6
|
32.5
|
1.0
|
|
N
|
P:ASN52
|
4.8
|
49.0
|
1.0
|
|
N
|
P:LEU50
|
4.8
|
42.0
|
1.0
|
|
CA
|
P:ASN57
|
4.9
|
30.8
|
1.0
|
|
CA
|
P:ASP53
|
4.9
|
43.2
|
1.0
|
|
O
|
P:ASP49
|
4.9
|
41.9
|
1.0
|
|
C
|
P:ASN51
|
4.9
|
49.0
|
1.0
|
|
N
|
P:GLY54
|
5.0
|
43.1
|
1.0
|
|
CB
|
P:ASN51
|
5.0
|
53.8
|
1.0
|
|
Calcium binding site 2 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 2 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca501
b:25.0
occ:1.00
|
OD1
|
P:ASP397
|
2.4
|
20.1
|
1.0
|
|
OD2
|
P:ASP371
|
2.4
|
24.9
|
1.0
|
|
O
|
P:GLY369
|
2.6
|
23.4
|
1.0
|
|
O
|
P:GLY367
|
2.6
|
21.6
|
1.0
|
|
OE1
|
P:GLN393
|
2.6
|
31.8
|
1.0
|
|
O
|
P:HOH2254
|
2.6
|
24.7
|
1.0
|
|
CG
|
P:ASP397
|
3.2
|
24.0
|
1.0
|
|
OD2
|
P:ASP397
|
3.2
|
23.9
|
1.0
|
|
CG
|
P:ASP371
|
3.5
|
26.3
|
1.0
|
|
C
|
P:GLY369
|
3.7
|
26.4
|
1.0
|
|
CD
|
P:GLN393
|
3.7
|
39.8
|
1.0
|
|
C
|
P:GLY367
|
3.8
|
24.8
|
1.0
|
|
C
|
P:THR368
|
3.9
|
25.9
|
1.0
|
|
O
|
P:THR368
|
4.0
|
24.2
|
1.0
|
|
N
|
P:ASP371
|
4.0
|
21.4
|
1.0
|
|
N
|
P:GLY369
|
4.1
|
24.6
|
1.0
|
|
CB
|
P:ASP371
|
4.1
|
22.5
|
1.0
|
|
C
|
P:GLY366
|
4.2
|
26.0
|
1.0
|
|
CA
|
P:THR368
|
4.3
|
25.2
|
1.0
|
|
N
|
P:GLY367
|
4.3
|
23.2
|
1.0
|
|
CG
|
P:GLN393
|
4.4
|
33.3
|
1.0
|
|
OD1
|
P:ASP371
|
4.4
|
24.2
|
1.0
|
|
CA
|
P:GLY366
|
4.4
|
22.7
|
1.0
|
|
O
|
P:GLY366
|
4.5
|
20.4
|
1.0
|
|
N
|
P:THR368
|
4.5
|
23.9
|
1.0
|
|
CA
|
P:GLY369
|
4.5
|
21.4
|
1.0
|
|
O
|
P:HOH2235
|
4.6
|
53.3
|
1.0
|
|
N
|
P:SER370
|
4.6
|
22.6
|
1.0
|
|
CA
|
P:SER370
|
4.6
|
26.5
|
1.0
|
|
CB
|
P:ASP397
|
4.6
|
23.4
|
1.0
|
|
O
|
P:HOH2237
|
4.7
|
50.8
|
1.0
|
|
CA
|
P:GLY367
|
4.7
|
22.6
|
1.0
|
|
C
|
P:SER370
|
4.8
|
23.9
|
1.0
|
|
CA
|
P:ASP371
|
4.8
|
22.3
|
1.0
|
|
CA
|
P:CA502
|
4.8
|
23.8
|
1.0
|
|
NE2
|
P:GLN393
|
4.8
|
39.2
|
1.0
|
|
O
|
P:ASP391
|
4.9
|
22.6
|
1.0
|
|
Calcium binding site 3 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 3 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca502
b:23.8
occ:1.00
|
O
|
P:GLY366
|
2.4
|
20.4
|
1.0
|
|
O
|
P:GLY349
|
2.4
|
23.3
|
1.0
|
|
OD1
|
P:ASP371
|
2.5
|
24.2
|
1.0
|
|
OD2
|
P:ASP353
|
2.5
|
19.0
|
1.0
|
|
O
|
P:GLY351
|
2.5
|
21.7
|
1.0
|
|
O
|
P:THR368
|
2.5
|
24.2
|
1.0
|
|
OD2
|
P:ASP371
|
3.0
|
24.9
|
1.0
|
|
CG
|
P:ASP371
|
3.1
|
26.3
|
1.0
|
|
CG
|
P:ASP353
|
3.5
|
21.0
|
1.0
|
|
C
|
P:GLY366
|
3.5
|
26.0
|
1.0
|
|
C
|
P:GLY351
|
3.6
|
24.7
|
1.0
|
|
C
|
P:GLY349
|
3.7
|
25.5
|
1.0
|
|
C
|
P:THR368
|
3.8
|
25.9
|
1.0
|
|
C
|
P:ALA350
|
3.8
|
26.8
|
1.0
|
|
O
|
P:ALA350
|
3.8
|
22.1
|
1.0
|
|
C
|
P:GLY367
|
4.0
|
24.8
|
1.0
|
|
N
|
P:GLY351
|
4.0
|
25.4
|
1.0
|
|
O
|
P:GLY367
|
4.1
|
21.6
|
1.0
|
|
N
|
P:ASP353
|
4.1
|
21.2
|
1.0
|
|
CB
|
P:ASP353
|
4.2
|
17.8
|
1.0
|
|
N
|
P:THR368
|
4.2
|
23.9
|
1.0
|
|
CA
|
P:GLY367
|
4.2
|
22.6
|
1.0
|
|
CA
|
P:ALA350
|
4.3
|
25.3
|
1.0
|
|
N
|
P:GLY367
|
4.3
|
23.2
|
1.0
|
|
C
|
P:GLY348
|
4.3
|
22.2
|
1.0
|
|
N
|
P:GLY349
|
4.3
|
22.4
|
1.0
|
|
CA
|
P:GLY351
|
4.4
|
25.3
|
1.0
|
|
N
|
P:ALA350
|
4.4
|
22.2
|
1.0
|
|
OD1
|
P:ASP353
|
4.4
|
20.7
|
1.0
|
|
CA
|
P:GLY369
|
4.4
|
21.4
|
1.0
|
|
N
|
P:ASN352
|
4.5
|
21.5
|
1.0
|
|
O
|
P:GLY348
|
4.5
|
19.5
|
1.0
|
|
N
|
P:GLY369
|
4.5
|
24.6
|
1.0
|
|
CA
|
P:GLY366
|
4.6
|
22.7
|
1.0
|
|
CB
|
P:ASP371
|
4.6
|
22.5
|
1.0
|
|
CA
|
P:THR368
|
4.6
|
25.2
|
1.0
|
|
CA
|
P:GLY348
|
4.6
|
20.4
|
1.0
|
|
CA
|
P:ASN352
|
4.6
|
23.3
|
1.0
|
|
CA
|
P:GLY349
|
4.6
|
23.1
|
1.0
|
|
C
|
P:GLY369
|
4.7
|
26.4
|
1.0
|
|
CA
|
P:CA503
|
4.7
|
24.5
|
1.0
|
|
O
|
P:GLY369
|
4.8
|
23.4
|
1.0
|
|
CA
|
P:CA501
|
4.8
|
25.0
|
1.0
|
|
C
|
P:ASN352
|
4.8
|
25.6
|
1.0
|
|
CA
|
P:ASP353
|
4.8
|
22.1
|
1.0
|
|
Calcium binding site 4 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 4 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca503
b:24.5
occ:1.00
|
O
|
P:GLY348
|
2.4
|
19.5
|
1.0
|
|
O
|
P:ALA350
|
2.5
|
22.1
|
1.0
|
|
OD2
|
P:ASP335
|
2.5
|
22.0
|
1.0
|
|
O
|
P:GLY333
|
2.5
|
21.7
|
1.0
|
|
OD1
|
P:ASP353
|
2.5
|
20.7
|
1.0
|
|
O
|
P:GLY331
|
2.5
|
23.8
|
1.0
|
|
OD2
|
P:ASP353
|
3.0
|
19.0
|
1.0
|
|
CG
|
P:ASP353
|
3.2
|
21.0
|
1.0
|
|
CG
|
P:ASP335
|
3.3
|
25.3
|
1.0
|
|
C
|
P:GLY348
|
3.5
|
22.2
|
1.0
|
|
C
|
P:GLY331
|
3.6
|
25.7
|
1.0
|
|
C
|
P:ALA350
|
3.7
|
26.8
|
1.0
|
|
C
|
P:GLY333
|
3.7
|
27.0
|
1.0
|
|
N
|
P:GLY333
|
4.0
|
27.8
|
1.0
|
|
C
|
P:GLY349
|
4.0
|
25.5
|
1.0
|
|
CB
|
P:ASP335
|
4.0
|
22.9
|
1.0
|
|
O
|
P:GLY349
|
4.0
|
23.3
|
1.0
|
|
OD1
|
P:ASP335
|
4.1
|
24.0
|
1.0
|
|
N
|
P:GLY331
|
4.1
|
23.3
|
1.0
|
|
N
|
P:ASP335
|
4.1
|
22.2
|
1.0
|
|
C
|
P:SER332
|
4.2
|
29.2
|
1.0
|
|
N
|
P:ALA350
|
4.2
|
22.2
|
1.0
|
|
CA
|
P:GLY349
|
4.3
|
23.1
|
1.0
|
|
N
|
P:GLY349
|
4.3
|
22.4
|
1.0
|
|
CA
|
P:GLY351
|
4.4
|
25.3
|
1.0
|
|
CA
|
P:GLY348
|
4.4
|
20.4
|
1.0
|
|
O
|
P:HOH2223
|
4.4
|
20.9
|
1.0
|
|
CA
|
P:SER332
|
4.4
|
28.0
|
1.0
|
|
N
|
P:SER332
|
4.4
|
25.4
|
1.0
|
|
N
|
P:GLY351
|
4.4
|
25.4
|
1.0
|
|
CA
|
P:GLY331
|
4.5
|
26.1
|
1.0
|
|
CA
|
P:GLY333
|
4.5
|
27.7
|
1.0
|
|
C
|
P:GLY330
|
4.6
|
23.9
|
1.0
|
|
CA
|
P:ALA350
|
4.6
|
25.3
|
1.0
|
|
CB
|
P:ASP353
|
4.6
|
17.8
|
1.0
|
|
N
|
P:ASN334
|
4.6
|
24.4
|
1.0
|
|
C
|
P:GLY351
|
4.6
|
24.7
|
1.0
|
|
O
|
P:SER332
|
4.7
|
27.6
|
1.0
|
|
O
|
P:GLY351
|
4.7
|
21.7
|
1.0
|
|
CA
|
P:CA502
|
4.7
|
23.8
|
1.0
|
|
CA
|
P:ASN334
|
4.7
|
24.3
|
1.0
|
|
CA
|
P:ASP335
|
4.8
|
22.7
|
1.0
|
|
C
|
P:ASN334
|
4.9
|
26.3
|
1.0
|
|
CA
|
P:GLY330
|
4.9
|
21.3
|
1.0
|
|
Calcium binding site 5 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 5 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca504
b:25.7
occ:1.00
|
OD2
|
P:ASP362
|
2.4
|
20.9
|
1.0
|
|
O
|
P:HOH2249
|
2.5
|
19.5
|
1.0
|
|
O
|
P:GLY360
|
2.5
|
24.9
|
1.0
|
|
O
|
P:ASP380
|
2.5
|
28.1
|
1.0
|
|
OD2
|
P:ASP387
|
2.5
|
22.3
|
1.0
|
|
O
|
P:GLY358
|
2.5
|
21.3
|
1.0
|
|
CG
|
P:ASP387
|
3.4
|
25.7
|
1.0
|
|
OD1
|
P:ASP387
|
3.4
|
27.7
|
1.0
|
|
CG
|
P:ASP362
|
3.4
|
25.6
|
1.0
|
|
C
|
P:ASP380
|
3.6
|
27.4
|
1.0
|
|
C
|
P:GLY358
|
3.7
|
28.1
|
1.0
|
|
C
|
P:GLY360
|
3.7
|
26.7
|
1.0
|
|
O
|
P:GLY359
|
3.8
|
25.3
|
1.0
|
|
C
|
P:GLY359
|
3.9
|
30.0
|
1.0
|
|
N
|
P:ASP362
|
4.0
|
24.1
|
1.0
|
|
CB
|
P:ASP362
|
4.1
|
20.4
|
1.0
|
|
CA
|
P:ASP380
|
4.1
|
26.1
|
1.0
|
|
C
|
P:GLY357
|
4.2
|
25.3
|
1.0
|
|
N
|
P:GLY358
|
4.2
|
23.8
|
1.0
|
|
N
|
P:GLY360
|
4.2
|
28.4
|
1.0
|
|
CB
|
P:ASP380
|
4.2
|
25.2
|
1.0
|
|
OD1
|
P:ASP362
|
4.3
|
24.0
|
1.0
|
|
O
|
P:GLY357
|
4.4
|
21.7
|
1.0
|
|
CA
|
P:GLY359
|
4.5
|
26.5
|
1.0
|
|
CA
|
P:GLY357
|
4.5
|
22.1
|
1.0
|
|
CA
|
P:GLY360
|
4.5
|
25.5
|
1.0
|
|
N
|
P:ALA361
|
4.5
|
23.7
|
1.0
|
|
N
|
P:GLY359
|
4.5
|
24.0
|
1.0
|
|
CA
|
P:GLY358
|
4.6
|
24.9
|
1.0
|
|
CA
|
P:CA505
|
4.6
|
25.8
|
1.0
|
|
CA
|
P:ALA361
|
4.6
|
25.8
|
1.0
|
|
N
|
P:SER381
|
4.7
|
25.3
|
1.0
|
|
O
|
P:HOH2247
|
4.7
|
43.1
|
1.0
|
|
O
|
P:HOH2245
|
4.7
|
21.5
|
1.0
|
|
C
|
P:ALA361
|
4.7
|
26.1
|
1.0
|
|
CB
|
P:ASP387
|
4.8
|
21.8
|
1.0
|
|
CA
|
P:ASP362
|
4.8
|
23.7
|
1.0
|
|
CA
|
P:SER381
|
4.8
|
24.1
|
1.0
|
|
CB
|
P:SER381
|
4.8
|
23.5
|
1.0
|
|
Calcium binding site 6 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 6 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca505
b:25.8
occ:1.00
|
O
|
P:GLY359
|
2.4
|
25.3
|
1.0
|
|
OD1
|
P:ASN344
|
2.5
|
22.4
|
1.0
|
|
OD1
|
P:ASP362
|
2.5
|
24.0
|
1.0
|
|
O
|
P:ASN340
|
2.5
|
23.6
|
1.0
|
|
O
|
P:GLY357
|
2.5
|
21.7
|
1.0
|
|
O
|
P:ALA342
|
2.6
|
20.9
|
1.0
|
|
OD2
|
P:ASP362
|
2.9
|
20.9
|
1.0
|
|
CG
|
P:ASP362
|
3.1
|
25.6
|
1.0
|
|
CG
|
P:ASN344
|
3.4
|
24.2
|
1.0
|
|
C
|
P:GLY357
|
3.6
|
25.3
|
1.0
|
|
C
|
P:GLY359
|
3.6
|
30.0
|
1.0
|
|
C
|
P:ASN340
|
3.7
|
28.4
|
1.0
|
|
C
|
P:ALA342
|
3.7
|
25.1
|
1.0
|
|
N
|
P:ASN340
|
3.9
|
24.6
|
1.0
|
|
C
|
P:GLY358
|
3.9
|
28.1
|
1.0
|
|
N
|
P:GLY359
|
4.0
|
24.0
|
1.0
|
|
O
|
P:GLY358
|
4.1
|
21.3
|
1.0
|
|
N
|
P:ASN344
|
4.1
|
23.6
|
1.0
|
|
N
|
P:ALA342
|
4.2
|
26.2
|
1.0
|
|
CB
|
P:ASN344
|
4.2
|
21.8
|
1.0
|
|
C
|
P:ASP341
|
4.2
|
29.9
|
1.0
|
|
ND2
|
P:ASN344
|
4.2
|
21.9
|
1.0
|
|
CA
|
P:GLY357
|
4.3
|
22.1
|
1.0
|
|
CA
|
P:GLY359
|
4.4
|
26.5
|
1.0
|
|
CA
|
P:GLY358
|
4.4
|
24.9
|
1.0
|
|
N
|
P:GLY358
|
4.4
|
23.8
|
1.0
|
|
O
|
P:ASP341
|
4.4
|
31.6
|
1.0
|
|
CA
|
P:ASP341
|
4.5
|
31.8
|
1.0
|
|
N
|
P:ASP341
|
4.5
|
28.8
|
1.0
|
|
CA
|
P:ASN340
|
4.5
|
27.4
|
1.0
|
|
C
|
P:GLY339
|
4.5
|
26.0
|
1.0
|
|
N
|
P:GLY360
|
4.6
|
28.4
|
1.0
|
|
CB
|
P:ASP362
|
4.6
|
20.4
|
1.0
|
|
CA
|
P:CA504
|
4.6
|
25.7
|
1.0
|
|
N
|
P:ALA343
|
4.6
|
24.8
|
1.0
|
|
OD1
|
P:ASN340
|
4.6
|
22.7
|
1.0
|
|
CA
|
P:ALA343
|
4.6
|
25.0
|
1.0
|
|
CA
|
P:ALA342
|
4.6
|
24.6
|
1.0
|
|
O
|
P:GLY360
|
4.6
|
24.9
|
1.0
|
|
C
|
P:ALA343
|
4.7
|
26.7
|
1.0
|
|
CA
|
P:GLY339
|
4.7
|
22.9
|
1.0
|
|
CA
|
P:GLY360
|
4.7
|
25.5
|
1.0
|
|
CA
|
P:ASN344
|
4.8
|
23.1
|
1.0
|
|
C
|
P:GLY360
|
4.9
|
26.7
|
1.0
|
|
Calcium binding site 7 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 7 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca506
b:24.9
occ:1.00
|
O
|
P:GLY285
|
2.4
|
23.3
|
1.0
|
|
O
|
P:THR324
|
2.5
|
24.3
|
1.0
|
|
OD2
|
P:ASP287
|
2.5
|
25.0
|
1.0
|
|
O
|
P:HOH2218
|
2.5
|
27.6
|
1.0
|
|
O
|
P:HOH2186
|
2.5
|
26.1
|
1.0
|
|
OE2
|
P:GLU326
|
2.6
|
25.1
|
1.0
|
|
OE1
|
P:GLU326
|
2.6
|
24.2
|
1.0
|
|
CD
|
P:GLU326
|
3.0
|
25.7
|
1.0
|
|
C
|
P:GLY285
|
3.5
|
26.3
|
1.0
|
|
CG
|
P:ASP287
|
3.6
|
24.8
|
1.0
|
|
C
|
P:THR324
|
3.7
|
25.5
|
1.0
|
|
N
|
P:GLY285
|
4.0
|
27.3
|
1.0
|
|
C
|
P:GLY284
|
4.1
|
27.9
|
1.0
|
|
N
|
P:ASP287
|
4.2
|
23.7
|
1.0
|
|
CB
|
P:ASP287
|
4.3
|
23.7
|
1.0
|
|
O
|
P:GLY284
|
4.3
|
26.8
|
1.0
|
|
CA
|
P:GLY285
|
4.4
|
25.4
|
1.0
|
|
N
|
P:ASN286
|
4.4
|
26.2
|
1.0
|
|
O
|
P:HOH2216
|
4.4
|
27.5
|
1.0
|
|
OD1
|
P:ASP287
|
4.5
|
23.1
|
1.0
|
|
CB
|
P:THR324
|
4.5
|
27.8
|
1.0
|
|
CA
|
P:ILE325
|
4.5
|
24.2
|
1.0
|
|
OD1
|
P:ASP282
|
4.5
|
25.5
|
1.0
|
|
N
|
P:ILE325
|
4.5
|
22.4
|
1.0
|
|
CA
|
P:ASN286
|
4.5
|
27.6
|
1.0
|
|
CG
|
P:GLU326
|
4.5
|
23.6
|
1.0
|
|
CA
|
P:THR324
|
4.6
|
27.3
|
1.0
|
|
CA
|
P:GLY284
|
4.7
|
30.0
|
1.0
|
|
N
|
P:GLU326
|
4.8
|
24.0
|
1.0
|
|
O
|
P:HOH2219
|
4.8
|
46.3
|
1.0
|
|
C
|
P:ASN286
|
4.8
|
24.4
|
1.0
|
|
N
|
P:THR324
|
4.9
|
25.3
|
1.0
|
|
CG2
|
P:THR324
|
5.0
|
27.8
|
1.0
|
|
CA
|
P:ASP287
|
5.0
|
24.3
|
1.0
|
|
Calcium binding site 8 out
of 8 in 1h71
Go back to
Calcium Binding Sites List in 1h71
Calcium binding site 8 out
of 8 in the Psychrophilic Protease From Pseudoalteromonas 'Tac II 18'
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Psychrophilic Protease From Pseudoalteromonas 'Tac II 18' within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
P:Ca507
b:26.9
occ:1.00
|
O
|
P:GLY284
|
2.4
|
26.8
|
1.0
|
|
OD1
|
P:ASP287
|
2.5
|
23.1
|
1.0
|
|
OG1
|
P:THR254
|
2.5
|
22.1
|
1.0
|
|
O
|
P:ASP252
|
2.5
|
28.6
|
1.0
|
|
O
|
P:ARG250
|
2.5
|
26.1
|
1.0
|
|
OD1
|
P:ASP282
|
2.6
|
25.5
|
1.0
|
|
OD2
|
P:ASP282
|
2.6
|
21.7
|
1.0
|
|
CG
|
P:ASP282
|
2.9
|
25.2
|
1.0
|
|
CG
|
P:ASP287
|
3.2
|
24.8
|
1.0
|
|
OD2
|
P:ASP287
|
3.4
|
25.0
|
1.0
|
|
CB
|
P:THR254
|
3.5
|
22.7
|
1.0
|
|
C
|
P:ARG250
|
3.6
|
29.7
|
1.0
|
|
C
|
P:GLY284
|
3.6
|
27.9
|
1.0
|
|
C
|
P:ASP252
|
3.7
|
33.0
|
1.0
|
|
N
|
P:THR254
|
3.8
|
26.6
|
1.0
|
|
N
|
P:ASP252
|
4.1
|
31.9
|
1.0
|
|
C
|
P:ALA251
|
4.2
|
31.4
|
1.0
|
|
N
|
P:GLY284
|
4.2
|
28.4
|
1.0
|
|
C
|
P:ASP253
|
4.2
|
30.8
|
1.0
|
|
CB
|
P:ARG250
|
4.3
|
28.4
|
1.0
|
|
CA
|
P:THR254
|
4.4
|
22.8
|
1.0
|
|
CA
|
P:GLY285
|
4.4
|
25.4
|
1.0
|
|
O
|
P:HOH2163
|
4.4
|
33.9
|
1.0
|
|
N
|
P:ALA251
|
4.4
|
29.5
|
1.0
|
|
CA
|
P:ASP253
|
4.4
|
30.7
|
1.0
|
|
N
|
P:GLY285
|
4.4
|
27.3
|
1.0
|
|
CA
|
P:ALA251
|
4.4
|
30.5
|
1.0
|
|
CB
|
P:ASP282
|
4.4
|
23.0
|
1.0
|
|
CA
|
P:ARG250
|
4.5
|
29.2
|
1.0
|
|
N
|
P:ASP253
|
4.5
|
29.6
|
1.0
|
|
CA
|
P:GLY284
|
4.6
|
30.0
|
1.0
|
|
O
|
P:HOH2186
|
4.6
|
26.1
|
1.0
|
|
CA
|
P:ASP252
|
4.6
|
33.1
|
1.0
|
|
CB
|
P:ASP287
|
4.6
|
23.7
|
1.0
|
|
CG2
|
P:THR254
|
4.7
|
22.8
|
1.0
|
|
C
|
P:GLY285
|
4.7
|
26.3
|
1.0
|
|
O
|
P:ALA251
|
4.8
|
33.1
|
1.0
|
|
OH
|
P:TYR256
|
4.9
|
23.4
|
1.0
|
|
N
|
P:GLY283
|
4.9
|
24.9
|
1.0
|
|
OD1
|
P:ASP252
|
5.0
|
39.7
|
1.0
|
|
O
|
P:ASP253
|
5.0
|
29.0
|
1.0
|
|
Reference:
N.Aghajari,
F.Van Petegem,
V.Villeret,
J.-P.Chessa,
C.Gerday,
R.Haser,
J.Van Beeumen.
Crystal Structures of A Psychrophilic Metalloprotease Reveal New Insights Into Catalysis By Cold-Adapted Proteases Proteins: Struct.,Funct., V. 50 636 2003GENET..
ISSN: ISSN 0887-3585
PubMed: 12577270
DOI: 10.1002/PROT.10264
Page generated: Mon Jul 7 15:33:11 2025
|
