Calcium in PDB 1i2e: Ribonuclease T1 V16A Mutant, Form I

All present enzymatic activity of Ribonuclease T1 V16A Mutant, Form I:
3.1.27.3;

The structure of Ribonuclease T1 V16A Mutant, Form I, PDB code: 1i2e was solved by S.De Vos, J.Backmann, J.Steyaert, R.Loris, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.00 / 1.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.229, 46.591, 49.768, 90.00, 90.00, 90.00
R / Rfree (%)	19 / 20.9

The binding sites of Calcium atom in the Ribonuclease T1 V16A Mutant, Form I (pdb code 1i2e). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Ribonuclease T1 V16A Mutant, Form I, PDB code: 1i2e:

Calcium binding site 1 out of 1 in the Ribonuclease T1 V16A Mutant, Form I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1 V16A Mutant, Form I within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca105 b:24.2 occ:1.00 O A:HOH203 2.4 23.6 1.0 O A:HOH205 2.4 30.7 1.0 O A:HOH202 2.4 26.5 1.0 OD2 A:ASP15 2.5 19.0 1.0 O A:HOH204 2.5 31.9 1.0 OD1 A:ASP15 2.6 19.6 1.0 O A:HOH274 2.7 43.0 1.0 CG A:ASP15 2.8 19.2 1.0 CB A:ASP15 4.3 18.8 1.0 O A:HOH201 4.3 29.4 1.0 OG A:SER12 4.4 19.4 1.0 O A:CYS10 4.6 19.9 1.0 O A:HOH243 4.7 45.1 1.0 O A:HOH208 4.8 27.7 1.0 N A:SER12 4.8 18.6 1.0 O A:HOH207 5.0 32.0 1.0

S.De Vos, J.Backmann, M.Prevost, J.Steyaert, R.Loris. Hydrophobic Core Manipulations in Ribonuclease T1 Biochemistry V. 40 10140 2001.
ISSN: ISSN 0006-2960
PubMed: 11513591
DOI: 10.1021/BI010565N