Calcium in PDB 1i2f: Ribonuclease T1 V16A Mutant, Form II

All present enzymatic activity of Ribonuclease T1 V16A Mutant, Form II:
3.1.27.3;

The structure of Ribonuclease T1 V16A Mutant, Form II, PDB code: 1i2f was solved by S.De Vos, J.Backmann, J.Steyaert, R.Loris, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	40.393, 42.773, 56.664, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 20.5

The binding sites of Calcium atom in the Ribonuclease T1 V16A Mutant, Form II (pdb code 1i2f). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ribonuclease T1 V16A Mutant, Form II, PDB code: 1i2f:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ribonuclease T1 V16A Mutant, Form II


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ribonuclease T1 V16A Mutant, Form II within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca106 b:16.8 occ:1.00 O A:HOH111 2.4 11.6 1.0 O A:HOH128 2.5 19.2 1.0 O A:HOH120 2.5 16.8 1.0 OD2 A:ASP15 2.5 9.6 1.0 O A:HOH132 2.6 36.1 1.0 O A:HOH119 2.6 13.2 1.0 OD1 A:ASP15 2.7 10.4 1.0 O A:HOH133 2.8 31.3 1.0 CG A:ASP15 2.9 8.4 1.0 O A:HOH117 4.4 20.8 1.0 CB A:ASP15 4.4 6.2 1.0 O A:HOH126 4.5 15.7 1.0 O A:CYS10 4.7 9.7 1.0 OG A:SER12 4.7 7.2 1.0 O A:HOH114 4.8 14.6 1.0 CA A:GLY65 4.9 14.7 1.0

Calcium binding site 2 out of 2 in the Ribonuclease T1 V16A Mutant, Form II


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ribonuclease T1 V16A Mutant, Form II within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca107 b:31.2 occ:1.00 O A:HOH150 2.3 34.7 1.0 OD1 A:ASP66 2.4 14.6 1.0 O A:HOH151 2.4 32.6 1.0 CG A:ASP66 3.4 15.6 1.0 OD2 A:ASP66 3.8 16.4 1.0 O A:SER64 4.4 17.9 1.0 CB A:ASP66 4.7 14.0 1.0 N A:ASP66 4.8 13.3 1.0 CA A:ASP66 4.9 12.6 1.0

S.De Vos, J.Backmann, M.Prevost, J.Steyaert, R.Loris. Hydrophobic Core Manipulations in Ribonuclease T1 Biochemistry V. 40 10140 2001.
ISSN: ISSN 0006-2960
PubMed: 11513591
DOI: 10.1021/BI010565N