Calcium in PDB 1jdc: Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1)

All present enzymatic activity of Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1):
3.2.1.60;

The structure of Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1), PDB code: 1jdc was solved by Y.Yoshioka, K.Hasegawa, Y.Matsuura, Y.Katsube, M.Kubota, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	10.00 / 1.90
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	65.100, 138.400, 50.700, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The binding sites of Calcium atom in the Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1) (pdb code 1jdc). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1), PDB code: 1jdc:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca451 b:13.9 occ:1.00 O A:GLY197 2.3 12.1 1.0 O A:HOH553 2.4 15.7 1.0 OD1 A:ASN116 2.4 13.3 1.0 O A:ASP154 2.5 15.8 1.0 OD2 A:ASP151 2.5 15.8 1.0 OD2 A:ASP162 2.6 13.7 1.0 OD1 A:ASP151 2.8 15.8 1.0 CG A:ASP151 3.0 16.2 1.0 C A:GLY197 3.3 12.4 1.0 CG A:ASN116 3.4 12.9 1.0 CA A:GLY197 3.6 12.8 1.0 CG A:ASP162 3.7 13.3 1.0 C A:ASP154 3.7 15.6 1.0 ND2 A:ASN116 3.8 11.3 1.0 CB A:ASP162 4.1 14.0 1.0 CA A:ARG155 4.2 15.6 1.0 O A:GLY153 4.4 15.7 1.0 C A:GLY153 4.4 15.6 1.0 O A:ASN116 4.4 12.1 1.0 N A:ARG155 4.4 15.2 1.0 CB A:ASP151 4.5 16.8 1.0 N A:TYR198 4.5 12.2 1.0 N A:ASP154 4.6 15.5 1.0 CB A:ASN116 4.7 13.2 1.0 O A:LEU163 4.7 14.5 1.0 NH1 A:ARG137 4.7 16.0 1.0 O A:HOH704 4.7 20.8 1.0 OD1 A:ASP162 4.7 13.6 1.0 CA A:ASP154 4.8 15.8 1.0 CA A:GLY153 4.8 15.5 1.0 N A:GLY153 4.9 15.3 1.0 CB A:ARG155 5.0 16.7 1.0

Calcium binding site 2 out of 2 in the Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Mutant (E219Q) Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltotetraose (Crystal Type 1) within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca452 b:59.1 occ:1.00 O A:GLN2 2.1 45.9 1.0 O A:HIS13 2.4 15.8 1.0 OD1 A:ASP1 2.5 53.4 1.0 OE2 A:GLU17 2.6 18.3 1.0 ND1 A:HIS13 3.0 22.0 1.0 OD1 A:ASP16 3.0 25.6 1.0 C A:GLN2 3.3 46.0 1.0 CE1 A:HIS13 3.6 22.4 1.0 C A:HIS13 3.6 16.4 1.0 CD A:GLU17 3.6 17.3 1.0 CG A:ASP1 3.7 53.4 1.0 C A:ASP1 3.8 50.8 1.0 O A:ASP1 3.8 51.0 1.0 CG A:HIS13 4.0 20.9 1.0 N A:GLN2 4.0 49.6 1.0 CA A:GLY14 4.1 17.5 1.0 CG A:ASP16 4.2 24.1 1.0 N A:ALA3 4.2 43.6 1.0 N A:GLY14 4.3 16.9 1.0 CG A:GLU17 4.3 16.1 1.0 CA A:ASP1 4.3 51.7 1.0 CA A:GLN2 4.3 48.0 1.0 CA A:ALA3 4.3 40.8 1.0 CB A:HIS13 4.5 19.0 1.0 OE1 A:GLU17 4.5 17.7 1.0 CB A:ASP1 4.5 52.8 1.0 NZ A:LYS108 4.6 25.6 1.0 OD2 A:ASP1 4.6 53.5 1.0 CA A:HIS13 4.7 16.7 1.0 NE2 A:HIS13 4.7 22.6 1.0 C A:GLY14 4.7 18.0 1.0 OD2 A:ASP16 4.7 26.4 1.0 N A:ASP16 4.8 17.5 1.0 CE A:LYS108 4.9 24.2 1.0 CB A:ALA3 4.9 41.3 1.0 N A:GLU17 4.9 15.4 1.0 CD2 A:HIS13 4.9 21.8 1.0 C A:ASP16 5.0 16.6 1.0

Y.Yoshioka, K.Hasegawa, Y.Matsuura, Y.Katsube, M.Kubota. Crystal Structures of A Mutant Maltotetraose-Forming Exo-Amylase Cocrystallized with Maltopentaose. J.Mol.Biol. V. 271 619 1997.
ISSN: ISSN 0022-2836
PubMed: 9281429
DOI: 10.1006/JMBI.1997.1222