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Calcium in PDB 1loy: X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate

Enzymatic activity of X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate

All present enzymatic activity of X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate:
3.1.27.3;

Protein crystallography data

The structure of X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate, PDB code: 1loy was solved by P.Mignon, J.Steyaert, R.Loris, P.Geerlings, S.Loverix, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 33.40 / 1.55
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 40.023, 44.812, 50.105, 90.00, 90.00, 90.00
R / Rfree (%) 18.9 / 21.8

Calcium Binding Sites:

The binding sites of Calcium atom in the X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate (pdb code 1loy). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate, PDB code: 1loy:

Calcium binding site 1 out of 1 in 1loy

Go back to Calcium Binding Sites List in 1loy
Calcium binding site 1 out of 1 in the X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of X-Ray Structure of the H40A/E58A Mutant of Ribonuclease T1 Complexed with 3'-Guanosine Monophosphate within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca105

b:21.9
occ:1.00
 O A:HOH216 2.3 20.4 1.0
O A:HOH218 2.4 28.3 1.0
OD2 A:ASP15 2.4 19.1 1.0
O A:HOH222 2.5 25.8 1.0
O A:HOH217 2.5 26.8 1.0
O A:HOH219 2.6 26.0 1.0
OD1 A:ASP15 2.6 17.3 1.0
CG A:ASP15 2.8 18.3 1.0
CB A:ASP15 4.3 13.5 1.0
OG A:SER12 4.5 17.3 1.0
O A:CYS10 4.6 18.4 1.0
O A:HOH224 4.6 27.1 1.0
O A:HOH220 4.7 20.0 1.0
O A:HOH261 4.7 37.9 1.0
N A:SER12 4.9 15.9 1.0

Reference:

P.Mignon, J.Steyaert, R.Loris, P.Geerlings, S.Loverix. A Nucleophile Activation Dyad in Ribonucleases. A Combined X-Ray Crystallographic/Ab Initio Quantum Chemical Study J.Biol.Chem. V. 277 36770 2002.
ISSN: ISSN 0021-9258
PubMed: 12122018
DOI: 10.1074/JBC.M206461200
Page generated: Mon Jul 7 16:57:42 2025

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