Calcium in PDB 1r8l: The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis

All present enzymatic activity of The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis:
3.2.1.89;

The structure of The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis, PDB code: 1r8l was solved by C.Ryttersgaard, J.Le Nours, L.Lo Leggio, C.T.Jorgensen, L.L.Christensen, M.Bjornvad, S.Larsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.67 / 2.60
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.910, 81.190, 104.880, 90.00, 98.53, 90.00
R / Rfree (%)	18.6 / 21.6

The binding sites of Calcium atom in the The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis (pdb code 1r8l). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis, PDB code: 1r8l:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca901 b:21.8 occ:1.00 OD1 A:ASP272 2.2 32.9 1.0 OD1 A:ASP370 2.3 29.2 1.0 O A:HIS276 2.4 26.6 1.0 O A:SER367 2.4 25.8 1.0 OD1 A:ASN278 2.4 25.6 1.0 OD1 A:ASP274 2.9 34.8 1.0 CB A:ASP274 3.1 33.2 1.0 CG A:ASP274 3.2 32.6 1.0 CG A:ASP272 3.3 33.1 1.0 CG A:ASP370 3.3 28.7 1.0 CG A:ASN278 3.5 26.1 1.0 C A:HIS276 3.5 26.7 1.0 C A:SER367 3.6 24.9 1.0 OD2 A:ASP370 3.7 33.7 1.0 N A:HIS276 3.8 28.7 1.0 OD2 A:ASP272 3.9 33.1 1.0 ND2 A:ASN278 4.0 26.2 1.0 CA A:HIS276 4.1 27.0 1.0 CB A:HIS276 4.1 26.7 1.0 CA A:ASP274 4.1 31.8 1.0 OD2 A:ASP274 4.2 31.4 1.0 N A:ASP274 4.2 32.6 1.0 N A:GLY275 4.2 29.9 1.0 CB A:ASP272 4.3 33.0 1.0 CA A:ALA368 4.4 22.2 1.0 N A:ALA368 4.4 23.2 1.0 CA A:SER367 4.5 25.6 1.0 C A:ASP274 4.5 31.1 1.0 C A:GLY277 4.5 26.8 1.0 CB A:SER367 4.6 23.9 1.0 N A:ASN278 4.6 27.0 1.0 CB A:ASP370 4.6 24.9 1.0 N A:GLY277 4.6 26.3 1.0 N A:SER367 4.6 27.4 1.0 O A:GLY277 4.7 25.9 1.0 CB A:ASN278 4.7 25.9 1.0 OG A:SER367 4.8 25.6 1.0 CA A:ASN278 4.8 26.4 1.0 CA A:ASP272 4.8 33.7 1.0 C A:GLY275 4.9 29.9 1.0 C A:ALA368 4.9 21.9 1.0 CA A:GLY277 5.0 26.4 1.0 N A:ASP370 5.0 23.2 1.0

Calcium binding site 2 out of 2 in the The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca902 b:25.9 occ:1.00 OD1 B:ASP272 2.3 34.4 1.0 OD1 B:ASP370 2.3 27.9 1.0 OD1 B:ASN278 2.3 22.9 1.0 O B:SER367 2.3 27.4 1.0 O B:HIS276 2.4 25.6 1.0 OD1 B:ASP274 3.0 32.4 1.0 CB B:ASP274 3.3 32.1 1.0 CG B:ASP272 3.3 33.2 1.0 CG B:ASP370 3.4 27.6 1.0 CG B:ASN278 3.4 23.8 1.0 CG B:ASP274 3.4 31.9 1.0 C B:SER367 3.5 27.0 1.0 C B:HIS276 3.5 25.1 1.0 OD2 B:ASP370 3.8 29.4 1.0 ND2 B:ASN278 3.9 23.3 1.0 N B:HIS276 3.9 28.8 1.0 OD2 B:ASP272 3.9 34.1 1.0 CA B:HIS276 4.1 25.7 1.0 CB B:HIS276 4.1 26.1 1.0 CA B:ALA368 4.3 25.1 1.0 CA B:ASP274 4.3 30.9 1.0 N B:ALA368 4.3 25.5 1.0 OD2 B:ASP274 4.3 31.7 1.0 CB B:ASP272 4.3 32.4 1.0 N B:ASP274 4.4 32.2 1.0 C B:GLY277 4.4 25.0 1.0 N B:GLY275 4.4 28.8 1.0 N B:ASN278 4.4 25.7 1.0 CA B:SER367 4.4 27.9 1.0 CB B:SER367 4.6 28.3 1.0 O B:GLY277 4.6 24.0 1.0 N B:GLY277 4.6 25.0 1.0 CB B:ASN278 4.6 23.0 1.0 C B:ASP274 4.6 30.6 1.0 CB B:ASP370 4.6 25.7 1.0 N B:SER367 4.7 29.3 1.0 CA B:ASN278 4.7 25.7 1.0 C B:ALA368 4.8 23.7 1.0 OG B:SER367 4.8 28.8 1.0 CA B:GLY277 4.9 24.8 1.0 CA B:ASP272 4.9 33.0 1.0 N B:ASP370 4.9 24.1 1.0 C B:GLY275 5.0 29.5 1.0

C.Ryttersgaard, J.Le Nours, L.Lo Leggio, C.T.Jorgensen, L.L.Christensen, M.Bjornvad, S.Larsen. The Structure of Endo-Beta-1,4-Galactanase From Bacillus Licheniformis in Complex with Two Oligosaccharide Products J.Mol.Biol. V. 341 107 2004.
ISSN: ISSN 0022-2836
PubMed: 15312766
DOI: 10.1016/J.JMB.2004.05.017