Calcium in PDB 2asp: Structure of Rabbit Actin in Complex with Reidispongiolide C

The structure of Structure of Rabbit Actin in Complex with Reidispongiolide C, PDB code: 2asp was solved by J.S.Allingham, A.Zampella, M.V.D'auria, I.Rayment, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	35.00 / 1.64
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	170.402, 54.689, 40.872, 90.00, 95.81, 90.00
R / Rfree (%)	16.3 / 19.3

The binding sites of Calcium atom in the Structure of Rabbit Actin in Complex with Reidispongiolide C (pdb code 2asp). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of Rabbit Actin in Complex with Reidispongiolide C, PDB code: 2asp:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of Rabbit Actin in Complex with Reidispongiolide C


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of Rabbit Actin in Complex with Reidispongiolide C within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca400 b:10.2 occ:1.00 O2B A:ATP425 2.3 8.5 1.0 O A:HOH886 2.4 10.5 1.0 O A:HOH783 2.4 9.7 1.0 O3G A:ATP425 2.4 9.4 1.0 O A:HOH788 2.4 9.9 1.0 O A:HOH787 2.4 9.8 1.0 O A:HOH767 2.7 12.6 1.0 PG A:ATP425 3.6 11.6 1.0 PB A:ATP425 3.6 10.2 1.0 O3B A:ATP425 3.9 10.2 1.0 O1G A:ATP425 3.9 10.6 1.0 O A:HOH800 4.2 14.3 1.0 OE1 A:GLN137 4.2 11.3 1.0 O3A A:ATP425 4.2 9.3 1.0 O1A A:ATP425 4.3 10.4 1.0 CA A:GLY13 4.3 12.2 1.0 NZ A:LYS18 4.3 11.7 1.0 CD A:GLN137 4.5 11.0 1.0 O A:HOH761 4.6 16.1 1.0 OD2 A:ASP154 4.6 14.8 1.0 OD1 A:ASP154 4.7 20.1 1.0 OD1 A:ASP11 4.7 9.3 1.0 PA A:ATP425 4.7 10.7 1.0 OD2 A:ASP11 4.7 8.8 1.0 O1B A:ATP425 4.9 9.8 1.0 O2G A:ATP425 4.9 12.9 1.0 O A:HOH768 4.9 23.9 1.0 CG A:GLN137 4.9 9.6 1.0 NE2 A:GLN137 5.0 10.7 1.0

Calcium binding site 2 out of 2 in the Structure of Rabbit Actin in Complex with Reidispongiolide C


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of Rabbit Actin in Complex with Reidispongiolide C within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca600 b:13.4 occ:1.00 O A:HOH743 2.4 13.4 1.0 OE1 A:GLN263 2.4 12.2 1.0 O1 A:EDO706 2.4 30.1 1.0 O A:HOH805 2.4 26.9 1.0 OG A:SER265 2.4 13.8 1.0 O A:HOH822 2.5 18.4 1.0 O2 A:EDO706 2.6 31.2 1.0 O A:HOH794 2.6 24.7 1.0 C2 A:EDO706 3.0 30.8 1.0 C1 A:EDO706 3.1 31.1 1.0 CD A:GLN263 3.4 12.0 1.0 CB A:SER265 3.5 11.7 1.0 NE2 A:GLN263 3.7 10.6 1.0 N A:SER265 4.2 11.5 1.0 CA A:SER265 4.4 11.6 1.0 O A:SER271 4.5 15.5 1.0 O A:GLU270 4.6 18.7 1.0 O A:HOH784 4.6 15.8 1.0 O A:HOH1052 4.6 37.7 1.0 O A:MET269 4.6 16.5 1.0 O A:HOH820 4.6 24.0 1.0 CG A:GLN263 4.7 8.8 1.0 O A:HOH808 4.8 24.6 1.0 C A:GLU270 5.0 17.4 1.0

J.S.Allingham, A.Zampella, M.V.D'auria, I.Rayment. Structures of Microfilament Destabilizing Toxins Bound to Actin Provide Insight Into Toxin Design and Activity Proc.Natl.Acad.Sci.Usa V. 102 14527 2005.
ISSN: ISSN 0027-8424
PubMed: 16192358
DOI: 10.1073/PNAS.0502089102