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Calcium in PDB 2gft: Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose

Enzymatic activity of Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose

All present enzymatic activity of Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose:
3.2.1.89;

Protein crystallography data

The structure of Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose, PDB code: 2gft was solved by D.Welner, J.Le Nours, L.De Maria, C.T.Jorgensen, L.L.H.Christensen, S.Larsen, L.Lo Leggio, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.00 / 2.30
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 50.273, 79.354, 103.906, 90.00, 100.56, 90.00
R / Rfree (%) 18 / 23.2

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose (pdb code 2gft). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose, PDB code: 2gft:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 2gft

Go back to Calcium Binding Sites List in 2gft
Calcium binding site 1 out of 2 in the Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca901

b:29.1
occ:1.00
 OD1 A:ASP274 2.2 32.1 1.0
O A:SER367 2.3 26.9 1.0
O A:HIS276 2.3 33.0 1.0
OD2 A:ASP370 2.3 26.8 1.0
OD1 A:ASN278 2.4 30.9 1.0
OD1 A:ASP272 2.4 34.6 1.0
CG A:ASP274 3.4 32.5 1.0
CG A:ASP370 3.4 27.2 1.0
C A:HIS276 3.4 33.2 1.0
C A:SER367 3.4 26.7 1.0
CG A:ASN278 3.5 31.3 1.0
CG A:ASP272 3.6 34.7 1.0
OD1 A:ASP370 3.7 29.4 1.0
OD2 A:ASP274 3.9 32.4 1.0
N A:HIS276 4.0 33.7 1.0
ND2 A:ASN278 4.0 31.3 1.0
CA A:HIS276 4.0 33.2 1.0
CB A:HIS276 4.1 33.3 1.0
OD2 A:ASP272 4.1 37.9 1.0
N A:ASP274 4.2 33.1 1.0
N A:ALA368 4.3 26.6 1.0
CA A:SER367 4.3 27.1 1.0
CA A:ALA368 4.3 26.1 1.0
C A:GLY277 4.4 32.3 1.0
N A:ASN278 4.5 32.3 1.0
CB A:SER367 4.5 26.6 1.0
CB A:ASP274 4.5 32.6 1.0
N A:GLY277 4.6 32.9 1.0
N A:SER367 4.6 27.9 1.0
O A:GLY277 4.6 32.1 1.0
N A:GLY273 4.6 33.3 1.0
CB A:ASP370 4.6 26.4 1.0
N A:GLY275 4.7 33.4 1.0
CB A:ASN278 4.7 32.2 1.0
CA A:ASN278 4.7 32.3 1.0
CA A:ASP274 4.7 33.2 1.0
CB A:ASP272 4.7 33.7 1.0
C A:ALA368 4.7 26.4 1.0
N A:VAL369 4.8 25.8 1.0
CA A:ASP272 4.9 33.5 1.0
N A:ASP370 4.9 26.7 1.0
CA A:GLY277 4.9 32.5 1.0
C A:ASP274 4.9 33.2 1.0
OG A:SER367 5.0 26.4 1.0
C A:ASP272 5.0 33.5 1.0
CG A:HIS276 5.0 33.2 1.0

Calcium binding site 2 out of 2 in 2gft

Go back to Calcium Binding Sites List in 2gft
Calcium binding site 2 out of 2 in the Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the E263A Nucleophile Mutant of Bacillus Licheniformis Endo-Beta-1,4-Galactanase in Complex with Galactotriose within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca902

b:28.3
occ:1.00
 OD2 B:ASP370 2.2 27.0 1.0
O B:SER367 2.3 26.6 1.0
OD1 B:ASP274 2.3 31.9 1.0
O B:HIS276 2.4 32.8 1.0
OD1 B:ASN278 2.4 30.5 1.0
OD1 B:ASP272 2.4 34.5 1.0
CG B:ASP370 3.3 27.2 1.0
CG B:ASP274 3.3 32.6 1.0
C B:SER367 3.4 27.0 1.0
CG B:ASN278 3.4 31.2 1.0
C B:HIS276 3.5 33.2 1.0
CG B:ASP272 3.6 34.6 1.0
OD1 B:ASP370 3.7 29.0 1.0
OD2 B:ASP274 3.8 32.5 1.0
ND2 B:ASN278 3.9 31.2 1.0
N B:HIS276 4.0 33.5 1.0
CA B:HIS276 4.1 33.3 1.0
OD2 B:ASP272 4.1 37.9 1.0
CB B:HIS276 4.2 33.5 1.0
N B:ASP274 4.2 32.9 1.0
N B:ALA368 4.3 26.6 1.0
CA B:ALA368 4.3 26.2 1.0
CA B:SER367 4.4 26.8 1.0
CB B:SER367 4.4 26.6 1.0
CB B:ASP274 4.5 32.6 1.0
N B:ASN278 4.5 32.1 1.0
C B:GLY277 4.5 32.2 1.0
CB B:ASP370 4.5 26.3 1.0
N B:SER367 4.6 27.5 1.0
N B:GLY273 4.6 33.4 1.0
N B:GLY277 4.6 32.8 1.0
CB B:ASN278 4.7 31.9 1.0
C B:ALA368 4.7 26.3 1.0
O B:GLY277 4.7 32.0 1.0
CA B:ASN278 4.7 32.2 1.0
N B:GLY275 4.7 33.5 1.0
CA B:ASP274 4.7 33.1 1.0
CB B:ASP272 4.7 33.8 1.0
N B:VAL369 4.8 26.2 1.0
N B:ASP370 4.8 26.9 1.0
CA B:ASP272 4.9 33.5 1.0
OG B:SER367 4.9 26.0 1.0
CA B:GLY277 5.0 32.4 1.0
C B:ASP274 5.0 33.3 1.0

Reference:

J.Le Nours, L.De Maria, D.Welner, C.T.Jorgensen, L.L.H.Christensen, S.Larsen, L.Lo Leggio. Oligosaccharide Binding of Bacillus Licheniformis Endo-Beta-1,4-Galactanase To Be Published.
Page generated: Fri Jul 12 10:45:44 2024

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