Calcium in PDB 2ost: The Structure of A Bacterial Homing Endonuclease : I-SSP6803I

The structure of The Structure of A Bacterial Homing Endonuclease : I-SSP6803I, PDB code: 2ost was solved by L.Zhao, R.P.Bonocora, D.A.Shub, B.L.Stoddard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	160.13 / 3.10
Space group	I 4 2 2
Cell size a, b, c (Å), α, β, γ (°)	143.777, 143.777, 319.179, 90.00, 90.00, 90.00
R / Rfree (%)	27.8 / 32.8

The binding sites of Calcium atom in the The Structure of A Bacterial Homing Endonuclease : I-SSP6803I (pdb code 2ost). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Structure of A Bacterial Homing Endonuclease : I-SSP6803I, PDB code: 2ost:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Structure of A Bacterial Homing Endonuclease : I-SSP6803I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure of A Bacterial Homing Endonuclease : I-SSP6803I within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca501 b:54.9 occ:1.00 OE1 A:GLN49 2.3 71.6 1.0 OD2 A:ASP36 2.3 66.1 1.0 O A:VAL50 2.3 77.4 1.0 OP1 Y:DA18 2.4 63.8 1.0 CG A:ASP36 3.2 67.2 1.0 OD1 A:ASP36 3.3 67.5 1.0 P Y:DA18 3.4 63.3 1.0 C A:VAL50 3.4 77.1 1.0 CD A:GLN49 3.5 72.2 1.0 O5' Y:DA18 3.5 63.5 1.0 C5' Y:DA18 3.8 63.6 1.0 N A:VAL50 4.0 75.4 1.0 OP2 Y:DA18 4.2 62.9 1.0 CA A:VAL50 4.2 76.2 1.0 NE2 A:GLN49 4.3 72.0 1.0 OP2 Y:DA19 4.3 65.3 1.0 CG A:LYS51 4.3 79.0 1.0 CG A:GLN49 4.5 73.1 1.0 N A:LYS51 4.5 78.2 1.0 CB A:ASP36 4.6 67.4 1.0 CB A:VAL50 4.6 75.9 1.0 O3' Y:DT17 4.7 62.9 1.0 CA A:LYS51 4.7 79.3 1.0 C A:GLN49 4.8 74.6 1.0 O3' Y:DA18 4.9 65.2 1.0

Calcium binding site 2 out of 2 in the The Structure of A Bacterial Homing Endonuclease : I-SSP6803I


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure of A Bacterial Homing Endonuclease : I-SSP6803I within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca502 b:59.0 occ:1.00 OP1 Z:DA47 2.4 55.1 1.0 OE1 B:GLN49 2.4 75.5 1.0 OD2 B:ASP36 2.5 68.6 1.0 O B:VAL50 2.6 77.8 1.0 O B:HOH506 2.6 56.7 1.0 O5' Z:DA47 2.8 53.8 1.0 P Z:DA47 3.1 54.7 1.0 CG B:ASP36 3.3 68.5 1.0 OD1 B:ASP36 3.4 68.6 1.0 CD B:GLN49 3.6 75.7 1.0 C5' Z:DA47 3.7 53.5 1.0 C B:VAL50 3.8 77.7 1.0 OP2 Z:DA47 3.9 54.6 1.0 OP2 Z:DG48 4.0 57.2 1.0 NE2 B:GLN49 4.1 75.9 1.0 N B:VAL50 4.4 76.1 1.0 O3' Z:DG46 4.4 56.0 1.0 C4' Z:DA47 4.5 53.5 1.0 CA B:VAL50 4.6 77.0 1.0 O3' Z:DA47 4.7 55.2 1.0 CG B:LYS51 4.7 79.2 1.0 CB B:ASP36 4.8 68.1 1.0 N B:LYS51 4.8 78.6 1.0 CG B:GLN49 4.8 75.0 1.0 P Z:DG48 4.8 57.1 1.0 C3' Z:DA47 4.9 54.3 1.0 CA B:LYS51 4.9 79.7 1.0 CB B:VAL50 5.0 76.9 1.0

L.Zhao, R.P.Bonocora, D.A.Shub, B.L.Stoddard. The Restriction Fold Turns to the Dark Side: A Bacterial Homing Endonuclease with A Pd-(D/E)-Xk Motif. Embo J. V. 26 2432 2007.
ISSN: ISSN 0261-4189
PubMed: 17410205
DOI: 10.1038/SJ.EMBOJ.7601672