Calcium in PDB 2wxt: Clostridium Perfringens Alpha-Toxin Strain NCTC8237

All present enzymatic activity of Clostridium Perfringens Alpha-Toxin Strain NCTC8237:
3.1.4.3;

The structure of Clostridium Perfringens Alpha-Toxin Strain NCTC8237, PDB code: 2wxt was solved by N.Justin, C.E.Naylor, A.K.Basak, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.06 / 2.00
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	60.180, 174.370, 79.530, 90.00, 90.00, 90.00
R / Rfree (%)	17.5 / 23.2

The structure of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 also contains other interesting chemical elements:

Cadmium	(Cd)	8 atoms
Zinc	(Zn)	1 atom

The binding sites of Calcium atom in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237 (pdb code 2wxt). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237, PDB code: 2wxt:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1380 b:22.8 occ:1.00 OD1 A:ASN297 2.2 32.3 1.0 O A:HOH2190 2.4 25.5 1.0 OD1 A:ASP273 2.8 29.2 1.0 CG A:ASN297 3.0 38.7 1.0 ND2 A:ASN297 3.2 41.0 1.0 CG A:ASP273 3.7 31.9 1.0 OD2 A:ASP273 4.0 34.0 1.0 CG A:MET300 4.1 28.6 1.0 O A:ASP298 4.2 27.8 1.0 CB A:ASN297 4.5 40.3 1.0 O A:THR272 4.5 19.7 1.0 C A:ASN297 4.9 35.3 1.0 CA A:ASN297 4.9 42.0 1.0

Calcium binding site 2 out of 2 in the Clostridium Perfringens Alpha-Toxin Strain NCTC8237


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Clostridium Perfringens Alpha-Toxin Strain NCTC8237 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1381 b:34.1 occ:1.00 OD1 A:ASP293 2.5 50.2 1.0 OD2 A:ASP293 2.8 52.1 1.0 CG A:ASP293 2.9 49.2 1.0 O A:ASN294 4.0 46.8 1.0 CB A:ASP293 4.4 43.3 1.0 O A:PRO295 4.6 59.7 1.0 O A:HOH2203 4.6 29.0 1.0 C A:ASN294 5.0 48.9 1.0

S.G.Vachieri, G.C.Clark, A.Alape-Giron, M.Flores-Diaz, N.Justin, C.E.Naylor, R.W.Titball, A.K.Basak. Comparison of A Nontoxic Variant of Clostridium Perfringens [Alpha]-Toxin with the Toxic Wild-Type Strain Acta Crystallogr.,Sect.D V. 66 1067 2010.
ISSN: ISSN 0907-4449
PubMed: 20944240
DOI: 10.1107/S090744491003369X