Calcium in PDB 2yc4: Intraflagellar Transport Complex 25-27 From Chlamydomonas

The structure of Intraflagellar Transport Complex 25-27 From Chlamydomonas, PDB code: 2yc4 was solved by S.Bhogaraju, M.Taschner, E.Lorentzen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.848 / 2.80
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	50.020, 68.300, 93.780, 90.00, 92.42, 90.00
R / Rfree (%)	22.54 / 31.58

The structure of Intraflagellar Transport Complex 25-27 From Chlamydomonas also contains other interesting chemical elements:

Chlorine	(Cl)	1 atom
Magnesium	(Mg)	1 atom

The binding sites of Calcium atom in the Intraflagellar Transport Complex 25-27 From Chlamydomonas (pdb code 2yc4). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Intraflagellar Transport Complex 25-27 From Chlamydomonas, PDB code: 2yc4:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Intraflagellar Transport Complex 25-27 From Chlamydomonas


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Intraflagellar Transport Complex 25-27 From Chlamydomonas within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:63.4 occ:1.00 OG1 A:THR35 2.4 55.0 1.0 O A:THR35 2.5 49.0 1.0 O A:ASN27 2.7 45.3 1.0 O A:LYS32 2.8 59.8 1.0 O A:ASN127 2.8 50.0 1.0 OD1 A:ASP30 3.0 48.4 1.0 C A:THR35 3.0 50.4 1.0 CB A:THR35 3.4 59.9 1.0 CA A:THR35 3.4 54.8 1.0 N A:THR35 3.5 62.2 1.0 C A:LYS32 3.6 57.3 1.0 C A:ASN127 3.8 46.6 1.0 CG A:ASP30 3.8 53.8 1.0 C A:ASN27 3.8 43.6 1.0 OD2 A:ASP30 3.9 55.0 1.0 N A:PHE36 4.0 56.1 1.0 CB A:LYS32 4.2 54.7 1.0 CA A:LYS32 4.2 50.9 1.0 N A:LYS32 4.3 56.2 1.0 CA A:ASN127 4.3 40.4 1.0 N A:ASP33 4.4 61.2 1.0 N A:ASN34 4.5 61.2 1.0 CA A:PHE36 4.5 48.2 1.0 C A:ASP33 4.5 60.6 1.0 CA A:ASN27 4.6 39.1 1.0 CG A:ARG128 4.6 60.1 1.0 CA A:ASP33 4.6 53.4 1.0 CB A:ASN127 4.7 42.4 1.0 CG2 A:THR35 4.7 57.1 1.0 C A:ASN34 4.7 60.5 1.0 CB A:ASN27 4.7 45.0 1.0 N A:MET28 4.8 36.7 1.0 N A:ARG128 4.8 46.7 1.0 CA A:MET28 4.9 33.9 1.0

Calcium binding site 2 out of 2 in the Intraflagellar Transport Complex 25-27 From Chlamydomonas


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Intraflagellar Transport Complex 25-27 From Chlamydomonas within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca201 b:67.7 occ:1.00 OG1 B:THR35 2.6 52.5 1.0 O B:ASN27 2.6 40.5 1.0 O B:LYS32 2.7 60.7 1.0 OD1 B:ASP30 2.7 57.4 1.0 O B:ASN127 2.8 54.5 1.0 O B:THR35 2.9 54.1 1.0 C B:THR35 3.4 52.9 1.0 C B:LYS32 3.5 59.8 1.0 CG B:ASP30 3.6 62.3 1.0 CB B:THR35 3.6 59.2 1.0 C B:ASN27 3.7 43.1 1.0 CA B:THR35 3.7 56.5 1.0 N B:THR35 3.7 62.1 1.0 OD2 B:ASP30 3.8 55.7 1.0 C B:ASN127 3.8 50.1 1.0 N B:LYS32 4.0 55.9 1.0 CA B:LYS32 4.1 54.2 1.0 CB B:LYS32 4.1 54.9 1.0 N B:PHE36 4.3 55.8 1.0 CG B:ARG128 4.4 67.8 1.0 N B:ASP33 4.4 57.7 1.0 CA B:ASN127 4.4 42.4 1.0 CA B:ASN27 4.5 36.5 1.0 N B:ASN34 4.7 61.3 1.0 CA B:ASP33 4.7 53.3 1.0 N B:MET28 4.7 38.7 1.0 C B:ASP33 4.7 61.8 1.0 CB B:ASN27 4.8 46.1 1.0 CA B:MET28 4.8 38.7 1.0 N B:ARG128 4.8 45.3 1.0 CA B:PHE36 4.8 47.4 1.0 CB B:ASN127 4.8 44.8 1.0 N B:GLY31 4.9 51.4 1.0 CG2 B:THR35 4.9 57.7 1.0 C B:MET28 4.9 40.4 1.0 C B:ASN34 5.0 62.7 1.0 N B:ASP30 5.0 58.1 1.0 C B:GLY31 5.0 60.0 1.0

S.Bhogaraju, M.Taschner, M.Morawetz, C.Basquin, E.Lorentzen. Crystal Structure of the Intraflagellar Transport Complex 25/27. Embo J. V. 30 1907 2011.
ISSN: ISSN 0261-4189
PubMed: 21505417
DOI: 10.1038/EMBOJ.2011.110