Calcium in PDB 3er9: Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp

All present enzymatic activity of Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp:
2.1.1.57; 2.7.7.19;

The structure of Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp, PDB code: 3er9 was solved by C.Li, H.Li, S.Zhou, T.L.Poulos, P.D.Gershon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.69 / 2.06
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	69.531, 91.977, 133.734, 90.00, 90.00, 90.00
R / Rfree (%)	20.8 / 25

The binding sites of Calcium atom in the Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp (pdb code 3er9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp, PDB code: 3er9:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca901 b:33.4 occ:1.00 O1A B:3AT800 2.3 34.2 1.0 O3G B:3AT800 2.4 36.4 1.0 O1B B:3AT800 2.4 34.5 1.0 O B:HOH1066 2.4 31.1 1.0 OD1 B:ASP202 2.4 42.2 1.0 OD1 B:ASP204 2.4 32.8 1.0 OD2 B:ASP204 2.4 30.1 1.0 O B:HOH1310 2.6 42.8 1.0 CG B:ASP204 2.8 30.6 1.0 CG B:ASP202 3.4 43.6 1.0 PB B:3AT800 3.6 36.4 1.0 PA B:3AT800 3.6 37.5 1.0 PG B:3AT800 3.7 35.1 1.0 OD2 B:ASP202 3.7 45.3 1.0 CA B:CA902 3.9 48.0 1.0 O3B B:3AT800 4.0 36.6 1.0 O B:HOH1298 4.2 0.5 1.0 O3A B:3AT800 4.2 37.0 1.0 CB B:ASP204 4.3 28.5 1.0 O2G B:3AT800 4.3 38.7 1.0 OG B:SER188 4.3 31.3 1.0 O B:HOH1036 4.4 23.5 1.0 O5' B:3AT800 4.4 38.4 1.0 O B:HOH1308 4.4 34.7 1.0 O B:ASP202 4.6 40.5 1.0 N B:ASP202 4.7 44.2 1.0 N B:SER188 4.7 29.6 1.0 N B:ASP204 4.7 30.4 1.0 C B:ASP202 4.7 39.7 1.0 CB B:ASP202 4.8 42.3 1.0 O2A B:3AT800 4.8 37.3 1.0 O2B B:3AT800 4.9 34.4 1.0 O1G B:3AT800 4.9 38.8 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Heterodimeric Vaccinia Virus Mrna Polyadenylate Polymerase Complex with Uu and 3'-Deoxy Atp within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca902 b:48.0 occ:1.00 OD1 B:ASP204 2.4 32.8 1.0 O B:HOH1311 2.4 45.2 1.0 O B:HOH1310 2.4 42.8 1.0 OD2 B:ASP202 2.4 45.3 1.0 O B:HOH1308 2.7 34.7 1.0 CG B:ASP202 3.4 43.6 1.0 CG B:ASP204 3.6 30.6 1.0 OD1 B:ASP202 3.7 42.2 1.0 OD1 B:ASP253 3.8 32.4 1.0 OD2 B:ASP253 3.9 31.6 1.0 CA B:CA901 3.9 33.4 1.0 CG B:ASP253 4.2 30.9 1.0 CB B:ASP204 4.3 28.5 1.0 CA B:ASP204 4.4 28.0 1.0 O1A B:3AT800 4.5 34.2 1.0 OD2 B:ASP204 4.5 30.1 1.0 O B:HOH1116 4.6 39.6 1.0 CB B:ASP202 4.7 42.3 1.0 N B:ASP204 4.8 30.4 1.0 O B:ILE203 5.0 32.3 1.0

C.Li, H.Li, S.Zhou, E.Sun, J.Yoshizawa, T.L.Poulos, P.D.Gershon. Polymerase Translocation with Respect to Single-Stranded Nucleic Acid: Looping or Wrapping of Primer Around A Poly(A) Polymerase Structure V. 17 680 2009.
ISSN: ISSN 0969-2126
PubMed: 19446524
DOI: 10.1016/J.STR.2009.03.012