Calcium in PDB 3evr: Crystal Structure of Calcium Bound Monomeric GCAMP2

The structure of Crystal Structure of Calcium Bound Monomeric GCAMP2, PDB code: 3evr was solved by Q.Wang, B.Shui, M.I.Kotlikoff, H.Sondermann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.65 / 2.00
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	121.995, 121.995, 97.831, 90.00, 90.00, 90.00
R / Rfree (%)	16.3 / 19

The binding sites of Calcium atom in the Crystal Structure of Calcium Bound Monomeric GCAMP2 (pdb code 3evr). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of Calcium Bound Monomeric GCAMP2, PDB code: 3evr:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in the Crystal Structure of Calcium Bound Monomeric GCAMP2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Calcium Bound Monomeric GCAMP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1 b:17.6 occ:1.00 OD2 A:ASP359 2.4 14.8 1.0 O A:THR365 2.4 15.3 1.0 OD1 A:ASN363 2.5 18.3 1.0 OD1 A:ASP361 2.5 17.6 1.0 OE1 A:GLU370 2.5 17.9 1.0 OD1 A:ASP367 2.6 29.6 1.0 OE2 A:GLU370 2.6 18.7 1.0 CD A:GLU370 2.9 17.2 1.0 CG A:ASN363 3.4 24.7 1.0 CG A:ASP361 3.4 22.0 1.0 CG A:ASP359 3.5 17.1 1.0 C A:THR365 3.6 12.7 1.0 CG A:ASP367 3.7 48.2 1.0 OD2 A:ASP361 3.8 28.5 1.0 ND2 A:ASN363 3.9 22.8 1.0 N A:ASP367 4.1 11.3 1.0 N A:THR365 4.2 15.6 1.0 OD1 A:ASP359 4.2 19.9 1.0 N A:ASN363 4.2 20.1 1.0 N A:ASP361 4.4 22.1 1.0 CA A:ASP359 4.4 16.2 1.0 CG A:GLU370 4.4 11.5 1.0 OG1 A:THR365 4.4 21.4 1.0 CB A:ASP359 4.4 15.0 1.0 CB A:ASN363 4.5 18.5 1.0 CA A:THR365 4.5 13.8 1.0 CB A:ASP367 4.5 25.6 1.0 N A:ILE366 4.5 15.6 1.0 CA A:ILE366 4.5 14.5 1.0 N A:GLY362 4.5 18.1 1.0 C A:ASP359 4.6 18.4 1.0 CB A:ASP361 4.6 23.3 1.0 OD2 A:ASP367 4.6 68.9 1.0 N A:ALA360 4.7 15.7 1.0 CA A:ASN363 4.7 20.6 1.0 N A:GLY364 4.7 20.3 1.0 O A:HOH590 4.8 28.4 1.0 C A:ILE366 4.8 15.9 1.0 CA A:ASP361 4.8 17.9 1.0 C A:ASN363 4.9 27.9 1.0 C A:ASP361 4.9 27.6 1.0 CA A:ASP367 5.0 11.5 1.0

Calcium binding site 2 out of 4 in the Crystal Structure of Calcium Bound Monomeric GCAMP2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Calcium Bound Monomeric GCAMP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca2 b:16.3 occ:1.00 OD1 A:ASP323 2.4 16.5 1.0 OD1 A:ASP327 2.5 15.8 1.0 O A:THR329 2.5 14.7 1.0 OD1 A:ASP325 2.5 14.7 1.0 O A:HOH855 2.5 17.3 1.0 OE2 A:GLU334 2.6 17.0 1.0 OE1 A:GLU334 2.6 14.3 1.0 CD A:GLU334 2.9 18.1 1.0 CG A:ASP325 3.4 15.8 1.0 CG A:ASP327 3.5 18.1 1.0 CG A:ASP323 3.5 16.3 1.0 OD2 A:ASP325 3.7 17.3 1.0 C A:THR329 3.7 23.5 1.0 OD2 A:ASP327 4.0 15.4 1.0 CA A:ASP323 4.1 19.9 1.0 N A:ASP327 4.2 12.2 1.0 N A:THR329 4.3 13.5 1.0 CB A:ASP323 4.3 14.5 1.0 O A:HOH31 4.3 22.8 1.0 CG A:GLU334 4.4 14.7 1.0 OD2 A:ASP323 4.4 15.1 1.0 C A:ASP323 4.5 18.9 1.0 CG2 A:THR329 4.5 15.2 1.0 CB A:ASP327 4.5 12.5 1.0 CA A:ILE330 4.5 12.3 1.0 CA A:THR329 4.5 14.3 1.0 N A:ASP325 4.6 17.4 1.0 N A:ILE330 4.6 13.1 1.0 N A:GLY326 4.7 17.4 1.0 CB A:ASP325 4.7 12.4 1.0 CA A:ASP327 4.7 13.8 1.0 O A:HOH483 4.7 20.2 1.0 N A:LYS324 4.8 16.9 1.0 N A:GLY328 4.8 13.2 1.0 N A:THR331 4.9 12.5 1.0 C A:ASP325 4.9 15.0 1.0 CA A:ASP325 4.9 15.5 1.0 C A:ASP327 4.9 17.8 1.0 O A:HOH480 4.9 21.3 1.0 O A:ASP323 5.0 13.3 1.0

Calcium binding site 3 out of 4 in the Crystal Structure of Calcium Bound Monomeric GCAMP2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Calcium Bound Monomeric GCAMP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca3 b:41.9 occ:1.00 O A:GLN438 2.5 41.5 1.0 OE1 A:GLU443 2.6 27.3 1.0 OD2 A:ASP434 2.6 44.0 1.0 O A:HOH856 2.6 37.7 1.0 OD1 A:ASP436 2.6 40.8 1.0 OD1 A:ASP432 2.6 45.9 1.0 OE2 A:GLU443 2.7 51.1 1.0 CD A:GLU443 3.0 73.4 1.0 CG A:ASP436 3.3 30.6 1.0 CG A:ASP434 3.4 36.9 1.0 C A:GLN438 3.6 34.9 1.0 CG A:ASP432 3.6 64.7 1.0 OD2 A:ASP436 3.6 37.7 1.0 OD1 A:ASP434 3.7 48.8 1.0 N A:ASP436 4.0 50.0 1.0 N A:GLN438 4.2 35.8 1.0 CA A:ASP432 4.3 51.3 1.0 OD2 A:ASP432 4.3 42.5 1.0 N A:ASP434 4.3 46.3 1.0 N A:GLY435 4.3 52.5 1.0 CB A:ASP436 4.3 29.7 1.0 CA A:VAL439 4.4 33.9 1.0 N A:VAL439 4.4 35.5 1.0 N A:ASN440 4.4 35.7 1.0 N A:ILE433 4.4 46.5 1.0 CB A:ASP432 4.4 55.1 1.0 CA A:GLN438 4.5 34.1 1.0 CG A:GLU443 4.5 42.7 1.0 C A:ASP432 4.6 57.8 1.0 CA A:ASP436 4.6 37.8 1.0 CB A:ASP434 4.7 52.8 1.0 ND2 A:ASN440 4.8 48.0 1.0 N A:GLY437 4.8 49.6 1.0 CA A:ASP434 4.8 68.6 1.0 CG A:ASN440 4.8 55.0 1.0 C A:VAL439 4.8 32.9 1.0 C A:ASP434 4.9 64.6 1.0 C A:ASP436 4.9 48.2 1.0 CB A:GLN438 5.0 33.9 1.0

Calcium binding site 4 out of 4 in the Crystal Structure of Calcium Bound Monomeric GCAMP2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Calcium Bound Monomeric GCAMP2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca4 b:40.0 occ:1.00 O A:TYR402 2.3 42.4 1.0 OE2 A:GLU407 2.5 32.9 1.0 OD1 A:ASP396 2.6 34.0 1.0 OD1 A:ASN400 2.6 42.0 1.0 O A:HOH593 2.6 40.8 1.0 OE1 A:GLU407 2.7 38.2 1.0 OD1 A:ASP398 2.8 68.1 1.0 CD A:GLU407 2.9 38.6 1.0 CG A:ASP398 3.2 76.2 1.0 OD2 A:ASP398 3.2 71.9 1.0 CG A:ASN400 3.4 51.2 1.0 C A:TYR402 3.5 35.7 1.0 CG A:ASP396 3.6 57.4 1.0 ND2 A:ASN400 3.8 43.7 1.0 N A:TYR402 4.2 29.2 1.0 N A:ASN400 4.2 41.9 1.0 O A:HOH453 4.3 38.5 1.0 OD2 A:ASP396 4.3 35.1 1.0 CA A:TYR402 4.3 26.8 1.0 N A:ASP398 4.4 43.4 1.0 CA A:ASP396 4.4 27.9 1.0 N A:GLY399 4.4 39.8 1.0 CB A:ASP398 4.4 49.6 1.0 CG A:GLU407 4.4 33.5 1.0 CB A:ASN400 4.5 44.0 1.0 N A:ILE403 4.5 27.3 1.0 CB A:ASP396 4.5 30.8 1.0 CA A:ILE403 4.6 27.6 1.0 C A:ASP396 4.6 35.6 1.0 N A:SER404 4.6 39.0 1.0 O A:HOH581 4.6 38.4 1.0 CA A:ASP398 4.7 42.9 1.0 CB A:TYR402 4.7 34.5 1.0 C A:ASP398 4.8 39.8 1.0 CA A:ASN400 4.8 32.6 1.0 N A:GLY401 4.8 39.8 1.0 N A:LYS397 4.9 39.7 1.0 O A:ASP396 5.0 34.2 1.0

Q.Wang, B.Shui, M.I.Kotlikoff, H.Sondermann. Structural Basis For Calcium Sensing By GCAMP2. Structure V. 16 1817 2008.
ISSN: ISSN 0969-2126
PubMed: 19081058
DOI: 10.1016/J.STR.2008.10.008