Atomistry » Calcium » PDB 3r3v-3rk2 » 3rbu
Atomistry »
  Calcium »
    PDB 3r3v-3rk2 »
      3rbu »

Calcium in PDB 3rbu: N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa

Enzymatic activity of N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa

All present enzymatic activity of N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa:
3.4.17.21;

Protein crystallography data

The structure of N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa, PDB code: 3rbu was solved by J.Tykvart, P.Sacha, C.Barinka, J.Starkova, T.Knedlik, J.Lubkowski, J.Konvalinka, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 28.46 / 1.60
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 101.247, 130.559, 158.897, 90.00, 90.00, 90.00
R / Rfree (%) 15.7 / 18.2

Other elements in 3rbu:

The structure of N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa also contains other interesting chemical elements:

Zinc (Zn) 2 atoms
Chlorine (Cl) 1 atom

Calcium Binding Sites:

The binding sites of Calcium atom in the N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa (pdb code 3rbu). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa, PDB code: 3rbu:

Calcium binding site 1 out of 1 in 3rbu

Go back to Calcium Binding Sites List in 3rbu
Calcium binding site 1 out of 1 in the N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of N-Terminally Avitev-Tagged Human Glutamate Carboxypeptidase II in Complex with 2-Pmpa within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca1753

b:16.4
occ:1.00
 OE2 A:GLU436 2.3 17.7 1.0
O A:TYR272 2.3 16.7 1.0
O A:THR269 2.4 16.5 1.0
OE1 A:GLU433 2.5 17.1 1.0
O A:HOH2005 2.5 17.2 1.0
OE2 A:GLU433 2.5 16.9 1.0
OG1 A:THR269 2.5 16.2 1.0
CD A:GLU433 2.8 16.2 1.0
C A:THR269 3.3 16.4 1.0
CB A:THR269 3.3 16.8 1.0
C A:TYR272 3.4 16.2 1.0
CD A:GLU436 3.4 18.0 1.0
CA A:THR269 3.9 17.3 1.0
OE1 A:GLU436 3.9 17.3 1.0
N A:TYR272 4.0 17.2 1.0
CA A:PRO273 4.2 17.1 1.0
CB A:ASP266 4.2 18.7 1.0
N A:PRO270 4.2 17.3 1.0
N A:ALA274 4.3 16.8 1.0
N A:PRO273 4.3 16.9 1.0
CG A:GLU433 4.3 17.1 1.0
CA A:TYR272 4.3 17.4 1.0
C A:PRO273 4.4 17.9 1.0
CA A:PRO270 4.4 17.3 1.0
N A:ASP266 4.5 16.2 1.0
N A:GLY271 4.5 17.4 1.0
O A:ASP266 4.6 18.6 1.0
O A:ALA264 4.6 19.5 1.0
N A:THR269 4.6 17.8 1.0
CG A:GLU436 4.7 15.9 1.0
CG2 A:THR269 4.7 17.8 1.0
C A:PRO270 4.7 17.2 1.0
OD2 A:ASP266 4.7 19.2 1.0
CB A:ALA274 4.9 19.1 1.0
CA A:ASP266 4.9 17.7 1.0
CB A:GLU436 4.9 16.1 1.0
C A:GLY271 5.0 19.3 1.0

Reference:

J.Tykvart, P.Sacha, C.Barinka, T.Knedlik, J.Starkova, J.Lubkowski, J.Konvalinka. Efficient and Versatile One-Step Affinity Purification of in Vivo Biotinylated Proteins: Expression, Characterization and Structure Analysis of Recombinant Human Glutamate Carboxypeptidase II. Protein Expr.Purif. V. 82 106 2012.
ISSN: ISSN 1046-5928
PubMed: 22178733
DOI: 10.1016/J.PEP.2011.11.016
Page generated: Tue Jul 8 16:17:53 2025

Last articles

Zn in 9UUO
Zn in 9UUS
Zn in 9W4R
Zn in 9VKW
Zn in 9W4S
Zn in 9VH1
Zn in 9RMX
Zn in 9RMU
Zn in 9QWN
Zn in 9U9Y
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy