Atomistry » Calcium » PDB 3whi-3ws5 » 3wno
Atomistry »
  Calcium »
    PDB 3whi-3ws5 »
      3wno »

Calcium in PDB 3wno: D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose

Enzymatic activity of D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose

All present enzymatic activity of D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose:
2.4.1.248;

Protein crystallography data

The structure of D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose, PDB code: 3wno was solved by N.Suzuki, Z.Fujimoto, Y.M.Kim, M.Momma, N.Kishine, R.Suzuki, M.Kobayashi, A.Kimura, K.Funane, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 30.47 / 1.90
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 61.928, 167.715, 174.861, 90.00, 90.00, 90.00
R / Rfree (%) 17 / 19.5

Other elements in 3wno:

The structure of D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose (pdb code 3wno). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose, PDB code: 3wno:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 3wno

Go back to Calcium Binding Sites List in 3wno
Calcium binding site 1 out of 2 in the D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca801

b:30.8
occ:1.00
 OE1 A:GLU424 2.1 28.9 1.0
OD1 A:ASP541 2.3 30.6 1.0
O A:THR443 2.4 33.1 1.0
O A:GLY446 2.4 35.1 1.0
OE1 A:GLU426 2.5 28.1 1.0
OE2 A:GLU426 2.7 30.7 1.0
O A:ASP541 2.7 25.7 1.0
CD A:GLU426 2.9 30.3 1.0
CD A:GLU424 3.3 29.8 1.0
CG A:ASP541 3.4 31.7 1.0
C A:THR443 3.5 33.7 1.0
C A:ASP541 3.6 26.8 1.0
C A:GLY446 3.6 33.1 1.0
OG1 A:THR443 3.8 32.6 1.0
CA A:ASP541 3.9 28.1 1.0
OE2 A:GLU424 4.0 33.6 1.0
N A:THR443 4.2 31.8 1.0
OD2 A:ASP541 4.3 32.4 1.0
CB A:ASP541 4.3 29.8 1.0
CB A:GLU424 4.3 28.3 1.0
CG A:GLU424 4.3 29.2 1.0
CA A:THR443 4.3 32.1 1.0
N A:GLY444 4.3 33.3 1.0
CA A:PHE447 4.4 30.9 1.0
N A:PHE447 4.4 30.0 1.0
N A:GLY446 4.4 34.1 1.0
CG A:GLU426 4.4 29.9 1.0
CA A:GLY444 4.5 35.3 1.0
CA A:GLY446 4.6 34.1 1.0
CB A:TYR442 4.6 35.9 1.0
N A:ALA425 4.6 26.3 1.0
CB A:THR443 4.7 32.9 1.0
CA A:GLU424 4.7 26.9 1.0
N A:SER542 4.7 25.2 1.0
CB A:PHE447 4.8 30.7 1.0
C A:GLY444 4.9 35.3 1.0
CB A:SER542 4.9 26.5 1.0
N A:GLU426 5.0 28.2 1.0

Calcium binding site 2 out of 2 in 3wno

Go back to Calcium Binding Sites List in 3wno
Calcium binding site 2 out of 2 in the D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of D308A Mutant of Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase Complexed with Cycloisomaltooctaose within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca809

b:26.3
occ:1.00
 OE1 B:GLU424 2.3 26.1 1.0
OD1 B:ASP541 2.3 23.8 1.0
O B:THR443 2.4 27.0 1.0
O B:GLY446 2.5 25.6 1.0
OE1 B:GLU426 2.6 27.6 1.0
O B:ASP541 2.7 20.3 1.0
OE2 B:GLU426 2.7 30.5 1.0
CD B:GLU426 3.0 28.6 1.0
CD B:GLU424 3.4 25.8 1.0
CG B:ASP541 3.4 23.9 1.0
C B:THR443 3.5 28.1 1.0
C B:ASP541 3.6 21.9 1.0
C B:GLY446 3.6 25.1 1.0
OG1 B:THR443 3.8 27.8 1.0
CA B:ASP541 3.9 21.7 1.0
OE2 B:GLU424 4.1 28.5 1.0
N B:THR443 4.1 27.0 1.0
CB B:GLU424 4.2 24.8 1.0
OD2 B:ASP541 4.3 25.2 1.0
CB B:ASP541 4.3 22.7 1.0
CA B:THR443 4.3 27.8 1.0
CG B:GLU424 4.3 25.4 1.0
CA B:PHE447 4.3 23.4 1.0
N B:GLY444 4.4 28.6 1.0
N B:PHE447 4.4 24.3 1.0
N B:GLY446 4.5 27.8 1.0
CA B:GLY444 4.5 29.2 1.0
N B:ALA425 4.5 23.2 1.0
CG B:GLU426 4.5 28.3 1.0
CB B:TYR442 4.6 27.0 1.0
CA B:GLU424 4.6 24.1 1.0
CA B:GLY446 4.7 26.2 1.0
CB B:PHE447 4.7 22.6 1.0
CB B:THR443 4.7 28.6 1.0
N B:SER542 4.7 21.0 1.0
C B:GLY444 4.9 29.4 1.0
CB B:SER542 4.9 22.0 1.0
N B:SER445 5.0 30.7 1.0
C B:TYR442 5.0 27.2 1.0

Reference:

N.Suzuki, Z.Fujimoto, Y.M.Kim, M.Momma, N.Kishine, R.Suzuki, S.Suzuki, S.Kitamura, M.Kobayashi, A.Kimura, K.Funane. Structural Elucidation of Cyclization Mechanism of Alpha-1,6-Glucan By Bacillus Circulans T-3040 Cycloisomaltooligosaccharide Glucanotransferase To Be Published.
Page generated: Sat Jul 13 21:14:57 2024

Last articles

Zn in 9MJ5
Zn in 9HNW
Zn in 9G0L
Zn in 9FNE
Zn in 9DZN
Zn in 9E0I
Zn in 9D32
Zn in 9DAK
Zn in 8ZXC
Zn in 8ZUF
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy