Calcium in PDB 4go9: Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris

All present enzymatic activity of Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris:
5.4.11.99;

The structure of Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris, PDB code: 4go9 was solved by A.Lipski, S.Ravaud, X.Robert, R.Haser, N.Aghajari, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.80 / 2.20
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	64.520, 84.990, 123.950, 90.00, 99.53, 90.00
R / Rfree (%)	16.6 / 21.9

The binding sites of Calcium atom in the Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris (pdb code 4go9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris, PDB code: 4go9:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:21.0 occ:1.00 O A:HOH1133 2.3 14.4 1.0 O A:ILE28 2.3 17.9 1.0 OD1 A:ASN24 2.4 27.6 1.0 OD1 A:ASP22 2.4 18.5 1.0 OD1 A:ASP26 2.4 19.6 1.0 O A:HOH1132 2.5 17.6 1.0 OD2 A:ASP30 2.5 19.1 1.0 CG A:ASP26 3.2 17.7 1.0 CG A:ASP22 3.4 23.0 1.0 CG A:ASN24 3.4 23.1 1.0 OD2 A:ASP26 3.4 24.9 1.0 CG A:ASP30 3.5 16.3 1.0 C A:ILE28 3.5 18.1 1.0 CB A:ASP30 3.8 15.1 1.0 ND2 A:ASN24 4.0 28.0 1.0 OD2 A:ASP22 4.1 22.3 1.0 CB A:ASP22 4.2 18.2 1.0 N A:THR23 4.2 19.3 1.0 N A:ASN24 4.2 19.6 1.0 N A:ILE28 4.3 18.8 1.0 O A:GLU75 4.3 19.6 1.0 C A:GLY29 4.3 16.9 1.0 CA A:ASP22 4.3 16.6 1.0 CA A:ILE28 4.3 16.8 1.0 O A:GLY29 4.4 15.4 1.0 CB A:ASP26 4.5 18.6 1.0 N A:GLY29 4.5 15.5 1.0 N A:ASP30 4.5 16.3 1.0 CB A:ILE28 4.6 16.4 1.0 CB A:ASN24 4.6 21.5 1.0 CA A:GLY29 4.6 14.3 1.0 N A:ASP26 4.6 20.0 1.0 OD1 A:ASP30 4.6 18.4 1.0 C A:ASP22 4.8 21.2 1.0 CA A:ASP30 4.8 16.6 1.0 CA A:ASN24 4.8 24.4 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Trehalulose Synthase Mutant, Mutb D415N, in Complex with Tris within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca7001 b:18.6 occ:1.00 OD1 B:ASP22 2.3 13.7 1.0 OD1 B:ASN24 2.3 23.2 1.0 O B:HOH7289 2.4 16.0 1.0 O B:ILE28 2.4 18.9 1.0 OD2 B:ASP30 2.4 13.0 1.0 OD1 B:ASP26 2.5 15.9 1.0 O B:HOH7511 2.6 17.3 1.0 CG B:ASP26 3.3 17.7 1.0 CG B:ASP22 3.3 13.5 1.0 CG B:ASN24 3.4 20.4 1.0 CG B:ASP30 3.5 14.7 1.0 C B:ILE28 3.6 17.5 1.0 OD2 B:ASP26 3.6 18.6 1.0 CB B:ASP30 3.9 13.9 1.0 OD2 B:ASP22 4.1 18.2 1.0 N B:ASN24 4.1 20.3 1.0 ND2 B:ASN24 4.1 18.2 1.0 N B:THR23 4.1 16.5 1.0 CB B:ASP22 4.2 15.9 1.0 CA B:ASP22 4.3 15.1 1.0 O B:GLU75 4.3 18.2 1.0 N B:ILE28 4.3 13.7 1.0 CA B:ILE28 4.4 14.8 1.0 C B:GLY29 4.4 18.6 1.0 CB B:ASN24 4.5 23.6 1.0 O B:GLY29 4.5 17.3 1.0 CB B:ASP26 4.5 21.3 1.0 N B:ASP30 4.6 14.3 1.0 CB B:ILE28 4.6 17.1 1.0 N B:GLY29 4.6 13.0 1.0 N B:ASP26 4.6 21.7 1.0 OD1 B:ASP30 4.6 12.5 1.0 CA B:ASN24 4.7 25.3 1.0 CA B:GLY29 4.7 10.6 1.0 C B:ASP22 4.7 15.5 1.0 O B:HOH7483 4.8 21.2 1.0 CA B:ASP30 4.8 15.8 1.0 N B:GLY25 4.9 18.6 1.0 C B:ASN24 4.9 19.5 1.0

A.Lipski, H.Watzlawick, S.Ravaud, X.Robert, R.Haser, R.Mattes, N.Aghajari. Crystal Structure of the Trehalulose Synthase Mutb, Mutant D415N, in Complex with Tris To Be Published 2013.