Calcium in PDB 4h7k: Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205

The structure of Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205, PDB code: 4h7k was solved by M.Okai, J.Ohtsuka, L.F.Imai, T.Mase, R.Moriuchi, M.Tsuda, K.Nagata, Y.Nagata, M.Tanokura, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.75
Space group	P 21 21 2
Cell size a, b, c (Å), α, β, γ (°)	50.538, 72.153, 73.584, 90.00, 90.00, 90.00
R / Rfree (%)	17.2 / 20.5

The structure of Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205 also contains other interesting chemical elements:

Chlorine

(Cl)

2 atoms

The binding sites of Calcium atom in the Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205 (pdb code 4h7k). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205, PDB code: 4h7k:

Calcium binding site 1 out of 1 in the Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Haloalkane Dehalogenase Linb I253M Mutant From Sphingobium Sp. MI1205 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:11.9 occ:1.00 O A:PRO175 2.3 11.4 1.0 O A:HOH522 2.3 13.9 1.0 O A:ILE178 2.3 12.2 1.0 O A:HOH549 2.4 15.6 1.0 C A:PRO175 3.4 11.8 1.0 C A:ILE178 3.5 12.3 1.0 N A:ILE178 4.2 11.7 1.0 N A:GLY176 4.3 11.9 1.0 CA A:ILE178 4.3 11.8 1.0 CA A:PRO175 4.3 12.2 1.0 CA A:GLY176 4.3 12.5 1.0 O A:ARG180 4.4 14.9 1.0 O A:HOH545 4.5 13.3 1.0 C A:GLY176 4.5 12.6 1.0 CB A:ILE178 4.5 11.5 1.0 N A:LEU179 4.5 13.2 1.0 CB A:PRO175 4.6 12.4 1.0 C A:LEU179 4.6 14.6 1.0 O A:LEU179 4.6 14.9 1.0 CA A:LEU179 4.7 14.1 1.0 O A:GLY176 4.7 13.4 1.0 N A:LEU177 4.9 12.1 1.0 O A:HOH684 4.9 26.6 1.0

M.Okai, J.Ohtsuka, L.F.Imai, T.Mase, R.Moriuchi, M.Tsuda, K.Nagata, Y.Nagata, M.Tanokura. Crystal Structure and Site-Directed Mutagenesis Analyses of Haloalkane Dehalogenase Linb From Sphingobium Sp. Strain MI1205. J.Bacteriol. V. 195 2642 2013.
ISSN: ISSN 0021-9193
PubMed: 23564170
DOI: 10.1128/JB.02020-12