Calcium in PDB 4i8g: Bovine Trypsin at 0.8 Resolution

All present enzymatic activity of Bovine Trypsin at 0.8 Resolution:
3.4.21.4;

The structure of Bovine Trypsin at 0.8 Resolution, PDB code: 4i8g was solved by Z.Dauter, D.Liebschner, M.Dauter, A.Brzuszkiewicz, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 0.80
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	54.220, 58.530, 66.450, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / 12

The binding sites of Calcium atom in the Bovine Trypsin at 0.8 Resolution (pdb code 4i8g). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Bovine Trypsin at 0.8 Resolution, PDB code: 4i8g:

Calcium binding site 1 out of 1 in the Bovine Trypsin at 0.8 Resolution


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Bovine Trypsin at 0.8 Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:5.5 occ:1.00 OE1 A:GLU70 2.3 6.1 1.0 O A:VAL75 2.3 6.1 1.0 O A:ASN72 2.3 5.5 1.0 OE2 A:GLU80 2.3 6.1 1.0 O A:HOH1002 2.3 7.0 1.0 O A:HOH1001 2.4 6.4 1.0 CD A:GLU70 3.3 5.3 1.0 CD A:GLU80 3.4 6.1 1.0 C A:VAL75 3.4 5.7 1.0 C A:ASN72 3.5 5.3 1.0 CG A:GLU80 3.7 7.2 1.0 OE2 A:GLU70 3.8 6.3 1.0 CA A:VAL76 4.1 6.7 1.0 N A:GLU77 4.2 7.0 1.0 N A:VAL76 4.2 6.5 1.0 N A:VAL75 4.2 5.5 1.0 OE1 A:GLU77 4.3 7.8 1.0 CA A:ILE73 4.3 5.3 1.0 N A:ILE73 4.3 5.4 1.0 N A:ASN72 4.4 5.5 1.0 CA A:ASN72 4.4 5.7 1.0 CA A:VAL75 4.4 5.6 1.0 O A:HOH1250 4.5 7.2 1.0 O A:HOH1377 4.5 9.7 0.4 CG A:GLU77 4.5 8.3 1.0 N A:ASP71 4.5 5.3 1.0 OE1 A:GLU80 4.5 6.5 1.0 C A:ILE73 4.5 5.3 1.0 CG A:GLU70 4.6 5.2 1.0 C A:VAL76 4.6 7.4 1.0 CA A:GLU70 4.7 5.0 1.0 CB A:GLU77 4.8 8.2 1.0 CD A:GLU77 4.8 9.2 1.0 CB A:ASN72 4.8 6.8 1.0 CB A:GLU70 4.8 5.0 1.0 N A:ASN74 4.9 5.0 0.6 N A:ASN74 4.9 5.4 0.4 O A:ILE73 4.9 6.3 1.0 C A:ASP71 4.9 5.1 1.0 O A:HOH1378 4.9 12.2 0.4

D.Liebschner, M.Dauter, A.Brzuszkiewicz, Z.Dauter. On the Reproducibility of Protein Crystal Structures: Five Atomic Resolution Structures of Trypsin. Acta Crystallogr.,Sect.D V. 69 1447 2013.
ISSN: ISSN 0907-4449
PubMed: 23897468
DOI: 10.1107/S0907444913009050