Calcium in PDB 4m9v: ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna

The structure of ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna, PDB code: 4m9v was solved by Y.Liu, Y.O.Olanrewaju, X.Zhang, X.Cheng, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.46 / 0.97
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	36.388, 96.093, 36.408, 90.00, 113.14, 90.00
R / Rfree (%)	13 / 14.3

The structure of ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna also contains other interesting chemical elements:

Zinc

(Zn)

4 atoms

The binding sites of Calcium atom in the ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna (pdb code 4m9v). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna, PDB code: 4m9v:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca103 b:8.5 occ:1.00 O B:HOH271 2.3 9.8 1.0 O A:HOH159 2.4 9.7 1.0 O B:HOH268 2.4 9.9 1.0 O B:HOH270 2.4 10.3 1.0 O A:HOH160 2.4 9.7 1.0 O A:HOH161 2.4 9.8 1.0 O B:HOH269 2.5 9.1 1.0 H21 A:DG8 3.2 10.3 1.0 H21 B:DG6 3.8 11.0 1.0 N2 A:DG8 4.0 8.6 1.0 O A:HOH162 4.1 17.3 1.0 H5' B:DG7 4.2 10.5 1.0 O A:HOH120 4.4 21.2 0.5 O A:HOH163 4.4 25.1 1.0 O A:HOH120 4.4 16.0 0.5 O4' B:DG7 4.4 8.6 1.0 H4' A:DC9 4.5 14.9 1.0 H22 A:DG8 4.5 10.3 1.0 N3 B:DG6 4.6 7.7 1.0 H4' B:DG6 4.6 9.1 1.0 N2 B:DG6 4.6 9.2 1.0 O A:HOH165 4.6 17.2 1.0 H4' B:DG7 4.6 10.7 1.0 N3 A:DG8 4.7 8.7 1.0 H5' A:DC9 4.7 15.9 1.0 O B:HOH204 4.7 17.7 1.0 O4' A:DC9 4.7 10.7 1.0 C2 A:DG8 4.8 8.2 1.0 O B:HOH217 4.8 13.7 1.0 O4' B:DG6 4.8 7.3 1.0 O B:HOH205 4.9 11.3 1.0 C4' B:DG7 4.9 8.9 1.0 H1' B:DG6 4.9 8.6 1.0 C5' B:DG7 5.0 8.8 1.0

Calcium binding site 2 out of 2 in the ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of ZFP57 Mutant (E182Q) in Complex with 5-Carboxylcytosine Dna within 5.0Å range: probe atom residue distance (Å) B Occ E:Ca103 b:8.3 occ:1.00 O E:HOH281 2.3 10.1 1.0 O D:HOH146 2.4 9.5 1.0 O E:HOH206 2.4 9.8 1.0 O D:HOH145 2.4 9.2 1.0 O D:HOH147 2.4 10.5 1.0 O E:HOH280 2.4 9.9 1.0 O E:HOH279 2.4 9.2 1.0 H21 D:DG8 3.1 10.3 1.0 H21 E:DG6 3.8 10.3 1.0 N2 D:DG8 4.0 8.6 1.0 O D:HOH148 4.1 17.9 1.0 H5' E:DG7 4.2 9.9 1.0 O E:HOH259 4.4 23.7 1.0 O D:HOH150 4.4 24.8 1.0 O4' E:DG7 4.4 8.6 1.0 O D:HOH120 4.5 28.6 1.0 H22 D:DG8 4.5 10.3 1.0 H4' D:DC9 4.5 14.9 0.6 H4' D:DC9 4.5 14.9 0.4 N3 E:DG6 4.6 7.7 1.0 N2 E:DG6 4.6 8.6 1.0 H4' E:DG6 4.6 9.1 1.0 H4' E:DG7 4.6 10.3 1.0 O D:HOH152 4.6 18.0 1.0 N3 D:DG8 4.7 8.5 1.0 H5' D:DC9 4.7 15.8 1.0 O E:HOH201 4.7 18.6 1.0 O4' D:DC9 4.7 10.8 1.0 C2 D:DG8 4.8 7.7 1.0 O E:HOH219 4.8 13.3 1.0 O4' E:DG6 4.8 7.3 1.0 O E:HOH203 4.9 11.1 1.0 C4' E:DG7 4.9 8.6 1.0 H1' E:DG6 4.9 8.6 1.0 C5' E:DG7 5.0 8.2 1.0

Y.Liu, Y.O.Olanrewaju, X.Zhang, X.Cheng. Dna Recognition of 5-Carboxylcytosine By A ZFP57 Mutant at An Atomic Resolution of 0.97 Angstrom. Biochemistry V. 52 9310 2013.
ISSN: ISSN 0006-2960
PubMed: 24236546
DOI: 10.1021/BI401360N