Calcium in PDB 5i9o: Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature

All present enzymatic activity of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature:
3.1.31.1;

The structure of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature, PDB code: 5i9o was solved by J.L.Sorenson, J.L.Schlessman, B.Garcia-Moreno E., with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	60.89 / 1.95
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	31.195, 78.933, 60.891, 90.00, 90.18, 90.00
R / Rfree (%)	20 / 23.8

The binding sites of Calcium atom in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature (pdb code 5i9o). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature, PDB code: 5i9o:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca201 b:39.3 occ:1.00 O B:HOH356 2.5 18.6 1.0 O4P B:THP202 2.6 11.1 1.0 OD1 B:ASP40 2.7 13.2 1.0 O B:HOH319 2.8 16.3 1.0 OD2 B:ASP21 2.9 8.4 1.0 O B:HOH335 3.1 27.4 1.0 O B:HOH331 3.4 26.4 1.0 O B:HOH340 3.4 15.9 1.0 P2 B:THP202 3.6 10.8 1.0 OE2 B:GLU43 3.6 36.2 1.0 NH2 B:ARG35 3.7 8.2 1.0 CG B:ASP40 3.8 11.6 1.0 CG B:ASP21 3.9 8.9 1.0 O5P B:THP202 3.9 10.9 1.0 O B:THR41 4.2 10.7 1.0 O6P B:THP202 4.2 11.6 1.0 OD2 B:ASP40 4.3 13.3 1.0 CB B:ASP21 4.3 9.1 1.0 N B:THR41 4.4 9.9 1.0 CZ B:ARG35 4.5 8.0 1.0 O B:HOH364 4.7 19.0 1.0 CA B:ASP40 4.8 9.6 1.0 CD B:GLU43 4.8 30.8 1.0 NE B:ARG35 4.8 8.2 1.0 CB B:ASP40 4.9 10.6 1.0 O5' B:THP202 4.9 10.6 1.0 OD1 B:ASP21 5.0 8.9 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca201 b:33.4 occ:1.00 OD2 A:ASP21 2.7 8.3 1.0 O5P A:THP202 2.7 10.9 1.0 O A:HOH307 2.7 30.0 1.0 OD1 A:ASP40 2.8 21.0 1.0 O A:HOH314 2.9 18.2 1.0 OE2 A:GLU43 3.4 41.2 1.0 NH2 A:ARG35 3.6 7.7 1.0 P2 A:THP202 3.7 9.4 1.0 CG A:ASP21 3.8 8.8 1.0 O A:HOH339 4.0 17.2 1.0 O4P A:THP202 4.0 8.8 1.0 CG A:ASP40 4.0 17.7 1.0 O A:THR41 4.1 13.9 1.0 CB A:ASP21 4.2 8.5 1.0 O6P A:THP202 4.2 9.7 1.0 N A:THR41 4.3 13.1 1.0 CZ A:ARG35 4.4 8.0 1.0 CD A:GLU43 4.4 35.2 1.0 CA A:ASP40 4.6 12.8 1.0 NE A:ARG35 4.7 8.2 1.0 OE1 A:GLU43 4.8 40.4 1.0 CB A:ASP40 4.8 14.8 1.0 OD1 A:ASP21 4.9 9.1 1.0 O A:HOH337 4.9 20.6 1.0 C A:ASP40 4.9 13.1 1.0 OD2 A:ASP40 5.0 20.3 1.0

J.L.Sorenson, J.L.Schlessman, B.Garcia-Moreno E.. Crystal Structure of Staphylococcal Nuclease Variant Delta+Phs L36K L103K at Cryogenic Temperature To Be Published.