Calcium in PDB 5mwc: Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State

The structure of Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State, PDB code: 5mwc was solved by K.M.Boyko, A.Y.Nikolaeva, D.A.Korzhenevskiy, T.V.Rakitina, V.O.Popov, O.M.Subach, N.V.Barykina, F.V.Subach, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	72.80 / 2.45
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	82.100, 82.100, 157.470, 90.00, 90.00, 90.00
R / Rfree (%)	23.9 / 31

The binding sites of Calcium atom in the Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State (pdb code 5mwc). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State, PDB code: 5mwc:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca401 b:84.2 occ:1.00 OE2 D:GLU208 2.3 81.6 1.0 OD1 D:ASP199 2.3 74.1 1.0 OD1 D:ASP197 2.3 69.1 1.0 O D:ARG203 2.3 82.1 1.0 O D:HOH520 2.3 63.1 1.0 CD D:GLU208 3.0 79.7 1.0 OE1 D:GLU208 3.0 86.6 1.0 CG D:ASP199 3.2 80.9 1.0 CG D:ASP197 3.5 70.2 1.0 C D:ARG203 3.5 80.5 1.0 OD2 D:ASP199 3.5 74.7 1.0 CB D:ASN201 4.1 65.4 1.0 OD2 D:ASP197 4.1 69.0 1.0 N D:ARG203 4.1 90.0 1.0 OD2 D:ASP205 4.1 76.9 1.0 N D:ASN201 4.2 77.2 1.0 CA D:ARG203 4.3 84.1 1.0 N D:ASP205 4.3 58.4 1.0 CA D:ASN201 4.4 76.3 1.0 C D:ASN201 4.5 87.9 1.0 CG D:GLU208 4.5 70.4 1.0 N D:ILE204 4.5 71.0 1.0 CB D:ASP199 4.6 82.3 1.0 CB D:ASP197 4.6 66.7 1.0 CA D:ILE204 4.6 62.5 1.0 CG D:ASP205 4.7 67.4 1.0 CE1 D:PHE167 4.7 63.1 1.0 O D:ASN201 4.7 88.7 1.0 CG D:ARG203 4.8 87.9 1.0 CB D:ASP205 4.8 60.1 1.0 N D:GLY202 4.8 85.8 1.0 CA D:ASP197 4.8 69.8 1.0 C D:ILE204 4.9 56.9 1.0 CD1 D:PHE167 4.9 57.4 1.0 N D:ASP199 5.0 85.5 1.0

Calcium binding site 2 out of 3 in the Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State within 5.0Å range: probe atom residue distance (Å) B Occ D:Ca402 b:75.0 occ:1.00 OD1 D:ASP165 2.3 77.4 1.0 OE2 D:GLU172 2.3 91.3 1.0 OD1 D:ASP163 2.3 81.0 1.0 OE1 D:GLU172 2.3 79.2 1.0 O D:PHE167 2.3 63.3 1.0 OD1 D:ASP161 2.3 67.0 1.0 CD D:GLU172 2.4 84.5 1.0 CG D:ASP165 3.1 72.2 1.0 OD2 D:ASP165 3.2 81.1 1.0 CG D:ASP161 3.4 61.7 1.0 CG D:ASP163 3.5 74.2 1.0 C D:PHE167 3.6 59.6 1.0 CG D:GLU172 3.6 82.8 1.0 CA D:ASP161 3.8 61.4 1.0 CB D:ASP161 4.0 61.6 1.0 OD2 D:ASP163 4.1 77.1 1.0 N D:LYS162 4.3 60.6 1.0 C D:ASP161 4.3 62.3 1.0 CA D:ILE168 4.3 65.4 1.0 N D:PHE167 4.4 57.5 1.0 N D:ILE168 4.4 62.6 1.0 OD2 D:ASP161 4.4 60.5 1.0 N D:ASP169 4.4 75.8 1.0 N D:ASP163 4.5 60.3 1.0 CB D:ASP165 4.5 67.9 1.0 CA D:PHE167 4.5 58.7 1.0 N D:ASP165 4.5 56.3 1.0 CB D:ASP163 4.7 70.0 1.0 CB D:GLU172 4.7 82.6 1.0 N D:GLY164 4.8 61.5 1.0 C D:ILE168 4.9 75.4 1.0 CB D:PHE167 4.9 57.4 1.0 CA D:ASP165 4.9 58.8 1.0 N D:GLY166 5.0 53.5 1.0 OD1 D:ASP169 5.0 78.2 1.0

Calcium binding site 3 out of 3 in the Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of the Genetically-Encoded Green Calcium Indicator Ntnc in Its Calcium Bound State within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:88.4 occ:1.00 OE1 A:GLU208 2.3 95.3 1.0 CD A:GLU208 3.4 0.8 1.0 OE2 A:GLU208 3.7 95.4 1.0 CA A:PHE206 4.2 1.0 1.0 N A:PHE206 4.6 0.0 1.0 O A:PHE206 4.7 0.4 1.0 CG A:GLU208 4.7 99.9 1.0 CB A:PHE206 4.8 1.0 1.0 C A:PHE206 5.0 0.4 1.0

O.M.Subach, N.V.Barykina, K.D.Piatkevich, V.P.Sotskov, M.A.Roshchina, T.A.Kunitsyna, A.Y.Malyshev, A.M.Varizhuk, G.E.Pozmogova, K.V.Anokhin, G.N.Enikolopov, K.M.Boyko, A.Y.Nikolaeva, D.A.Korzhenevskiy, T.V.Rakitina, V.O.Popov, F.V.Subach. Enchanced Variant of Genetically-Encoded Green Calcium Indicator Ntnc To Be Published.