Atomistry » Calcium » PDB 6ai0-6b40 » 6aja
Atomistry »
  Calcium »
    PDB 6ai0-6b40 »
      6aja »

Calcium in PDB 6aja: Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+

Enzymatic activity of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+

All present enzymatic activity of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+:
1.1.1.42;

Protein crystallography data

The structure of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+, PDB code: 6aja was solved by X.Wang, D.K.Inaoka, T.Shiba, E.O.Balogun, N.Ziebart, S.Allman, Y.Watanabe, T.Nozaki, M.Boshart, F.Bringaud, S.Harada, K.Kita, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.93 / 2.85
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 120.894, 70.455, 122.052, 90.00, 113.39, 90.00
R / Rfree (%) 19.8 / 28.8

Calcium Binding Sites:

The binding sites of Calcium atom in the Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+ (pdb code 6aja). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+, PDB code: 6aja:
Jump to Calcium binding site number: 1; 2; 3; 4;

Calcium binding site 1 out of 4 in 6aja

Go back to Calcium Binding Sites List in 6aja
Calcium binding site 1 out of 4 in the Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+ within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca502

b:86.6
occ:1.00
OD1 A:ASP274 2.7 58.4 1.0
O A:ASP274 2.8 57.1 1.0
OD2 B:ASP251 3.0 66.0 1.0
OD1 A:ASP278 3.1 74.1 1.0
CG A:ASP278 3.6 75.6 1.0
CG A:ASP274 3.6 59.1 1.0
C A:ASP274 3.7 54.3 1.0
OD2 A:ASP278 3.7 79.3 1.0
CA A:ASP274 4.0 54.3 1.0
CG B:ASP251 4.0 64.5 1.0
CB B:ASP251 4.3 59.8 1.0
OD2 A:ASP274 4.4 58.6 1.0
CB A:ASP274 4.4 57.1 1.0
NH2 A:ARG132 4.6 44.9 1.0
CB A:ASP278 4.7 71.7 1.0
N A:VAL275 4.9 51.5 1.0

Calcium binding site 2 out of 4 in 6aja

Go back to Calcium Binding Sites List in 6aja
Calcium binding site 2 out of 4 in the Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+ within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca505

b:66.0
occ:1.00
OD1 B:ASP274 2.5 44.1 1.0
OD2 A:ASP251 2.8 66.8 1.0
OD1 B:ASP278 3.1 50.9 1.0
CG B:ASP274 3.7 41.9 1.0
CG B:ASP278 3.9 50.3 1.0
CG A:ASP251 3.9 63.0 1.0
OD2 B:ASP278 4.0 49.5 1.0
O B:ASP274 4.2 50.2 1.0
OD2 B:ASP274 4.3 41.1 1.0
CB A:ASP251 4.5 57.9 1.0
C B:ASP274 4.9 45.0 1.0
CB B:ASP274 4.9 41.4 1.0
OD1 A:ASP251 4.9 64.7 1.0
CA B:ASP274 5.0 42.1 1.0

Calcium binding site 3 out of 4 in 6aja

Go back to Calcium Binding Sites List in 6aja
Calcium binding site 3 out of 4 in the Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+ within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca502

b:75.2
occ:1.00
OD2 C:ASP278 2.6 66.3 1.0
OD1 C:ASP274 3.0 54.8 1.0
O C:ASP274 3.4 53.0 1.0
CG C:ASP278 3.5 63.9 1.0
CG C:ASP274 3.6 51.8 1.0
OD1 C:ASP278 3.6 62.2 1.0
OD2 C:ASP274 3.8 51.3 1.0
CB D:ASP251 3.8 56.9 1.0
CG D:ASP251 3.8 60.6 1.0
OD2 D:ASP251 4.1 62.8 1.0
OD1 D:ASP251 4.2 61.4 1.0
C C:ASP274 4.3 51.0 1.0
CA C:ASP274 4.7 49.1 1.0
CB C:ASP274 4.7 50.9 1.0
CB C:ASP278 4.9 58.6 1.0

Calcium binding site 4 out of 4 in 6aja

Go back to Calcium Binding Sites List in 6aja
Calcium binding site 4 out of 4 in the Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Crystal Structure of Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase in Complex with Nadph, Alpha-Ketoglutarate and CA2+ within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ca502

b:66.0
occ:1.00
O5 D:AKG503 2.5 83.1 1.0
O2 D:AKG503 2.6 75.1 1.0
OD1 D:ASP274 2.6 55.7 1.0
OD2 D:ASP278 2.8 50.9 1.0
C1 D:AKG503 3.1 81.4 1.0
C2 D:AKG503 3.2 82.5 1.0
CG D:ASP274 3.5 57.3 1.0
NH2 D:ARG109 3.6 54.6 1.0
CB C:ASP251 3.7 58.6 1.0
O D:ALA307 3.8 55.8 1.0
OD2 D:ASP274 3.8 63.3 1.0
OD2 C:ASP251 3.8 77.7 1.0
CG C:ASP251 3.9 69.7 1.0
CG D:ASP278 4.0 50.3 1.0
O D:ASP274 4.0 46.2 1.0
O1 D:AKG503 4.1 85.6 1.0
OD1 D:ASP278 4.4 49.6 1.0
NH2 D:ARG132 4.4 58.8 1.0
C5N D:NAP501 4.5 49.6 1.0
CA D:ASP274 4.6 50.8 1.0
C D:ASP274 4.6 49.0 1.0
CB D:ASP274 4.6 53.3 1.0
C3 D:AKG503 4.7 83.6 1.0
C4N D:NAP501 4.7 48.3 1.0
OD1 C:ASP251 4.7 73.4 1.0
NZ C:LYS211 4.8 55.6 1.0
CZ D:ARG109 4.9 52.6 1.0
C D:ALA307 4.9 53.3 1.0

Reference:

X.Wang, D.K.Inaoka, T.Shiba, E.O.Balogun, N.Ziebart, S.Allman, Y.Watanabe, T.Nozaki, M.Boshart, F.Bringaud, S.Harada, K.Kita. Biochemical Characterization of A Novel Trypanosoma Brucei Glycosomal Isocitrate Dehydrogenase with Dual Coenzyme Specificity (Nadp+/Nad+) To Be Published.
Page generated: Wed Jul 9 12:28:39 2025

Last articles

Mg in 8F4E
Mg in 8F4C
Mg in 8F4D
Mg in 8F2K
Mg in 8F3C
Mg in 8F2Y
Mg in 8F2W
Mg in 8F1K
Mg in 8F1V
Mg in 8F1S
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy