Calcium in PDB 6iyg: The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose

All present enzymatic activity of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose:
3.2.1.60;

The structure of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose, PDB code: 6iyg was solved by Z.F.Li, X.F.Ban, Z.Q.Zhang, C.M.Li, Z.B.Gu, T.C.Jin, Y.L.Li, Y.H.Shang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.44 / 1.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.280, 64.570, 168.910, 90.00, 90.00, 90.00
R / Rfree (%)	16.7 / 18.6

The binding sites of Calcium atom in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose (pdb code 6iyg). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose, PDB code: 6iyg:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:25.9 occ:1.00 OD1 A:ASP1 2.1 41.2 1.0 OE1 A:GLU17 2.2 28.9 1.0 OD1 A:ASP16 2.3 23.6 1.0 O A:GLN2 2.3 30.0 1.0 O A:HIS13 2.4 25.8 1.0 ND1 A:HIS13 2.5 25.3 1.0 CG A:ASP1 3.2 39.3 1.0 CG A:ASP16 3.4 31.1 1.0 CE1 A:HIS13 3.4 31.4 1.0 CD A:GLU17 3.4 29.0 1.0 C A:HIS13 3.4 25.9 1.0 HE1 A:HIS13 3.4 37.7 1.0 HB3 A:HIS13 3.5 31.8 1.0 C A:GLN2 3.5 36.8 1.0 OD2 A:ASP1 3.6 47.8 1.0 HG2 A:GLU17 3.6 27.1 1.0 CG A:HIS13 3.6 25.6 1.0 HZ1 A:LYS108 3.6 50.0 1.0 HA3 A:GLY14 3.7 32.0 1.0 OD2 A:ASP16 3.7 33.7 1.0 H A:GLN2 3.8 45.3 1.0 HA A:ALA3 3.8 36.4 1.0 H A:ASP16 3.8 29.5 1.0 CB A:HIS13 3.9 26.5 1.0 N A:GLN2 4.0 37.7 1.0 HZ3 A:LYS108 4.0 50.0 1.0 CG A:GLU17 4.1 22.5 1.0 NZ A:LYS108 4.2 41.6 1.0 N A:GLY14 4.3 23.7 1.0 CA A:HIS13 4.3 26.8 1.0 CA A:GLN2 4.3 39.8 1.0 HA A:ASP1 4.3 47.3 1.0 OE2 A:GLU17 4.3 28.0 1.0 CA A:GLY14 4.4 26.6 1.0 C A:ASP1 4.4 42.2 1.0 HE2 A:LYS108 4.4 40.7 1.0 N A:ALA3 4.4 34.9 1.0 CB A:ASP1 4.5 41.5 1.0 O A:HOH979 4.5 39.7 1.0 HG3 A:GLU17 4.5 27.1 1.0 HB3 A:GLN2 4.6 53.1 1.0 NE2 A:HIS13 4.6 32.1 1.0 CA A:ALA3 4.6 30.3 1.0 N A:ASP16 4.6 24.6 1.0 CA A:ASP1 4.7 39.4 1.0 CD2 A:HIS13 4.7 29.5 1.0 CB A:ASP16 4.7 24.1 1.0 HA A:HIS13 4.8 32.2 1.0 H A:GLY15 4.8 28.3 1.0 N A:GLU17 4.8 22.6 1.0 C A:ASP16 4.8 23.4 1.0 HB2 A:HIS13 4.9 31.8 1.0 C A:GLY14 4.9 26.2 1.0 H A:GLU17 4.9 27.1 1.0 HA A:GLU17 4.9 24.3 1.0 HB2 A:ASP1 4.9 49.9 1.0 HZ2 A:LYS108 4.9 50.0 1.0 CE A:LYS108 4.9 33.9 1.0 CA A:ASP16 5.0 22.7 1.0

Calcium binding site 2 out of 2 in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotetraose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca603 b:23.3 occ:1.00 O A:HOH739 2.3 24.5 1.0 O A:GLY197 2.3 23.4 1.0 O A:ASP154 2.3 23.9 1.0 OD1 A:ASN116 2.4 22.8 1.0 OD2 A:ASP151 2.4 24.5 1.0 OD2 A:ASP162 2.6 25.3 1.0 OD1 A:ASP151 2.6 23.6 1.0 CG A:ASP151 2.8 24.0 1.0 C A:GLY197 3.3 21.4 1.0 HA A:ARG155 3.4 31.1 1.0 CG A:ASN116 3.4 21.2 1.0 HB3 A:ASP162 3.5 29.4 1.0 HA2 A:GLY197 3.5 28.1 1.0 HA3 A:GLY197 3.5 28.1 1.0 C A:ASP154 3.6 24.1 1.0 CG A:ASP162 3.6 24.3 1.0 CA A:GLY197 3.7 23.4 1.0 ND2 A:ASN116 3.9 23.5 1.0 HH11 A:ARG137 4.0 31.4 1.0 CB A:ASP162 4.1 24.5 1.0 HD21 A:ASN116 4.2 28.2 1.0 H A:GLY153 4.2 30.5 1.0 HD22 A:ASN116 4.2 28.2 1.0 O A:GLY153 4.2 25.8 1.0 CA A:ARG155 4.2 25.9 1.0 C A:GLY153 4.2 25.1 1.0 HD2 A:ARG137 4.3 31.9 1.0 N A:ARG155 4.3 24.2 1.0 CB A:ASP151 4.3 25.5 1.0 N A:ASP154 4.4 23.7 1.0 HA3 A:GLY153 4.4 31.1 1.0 O A:ASN116 4.4 23.4 1.0 O A:HOH746 4.4 24.5 1.0 HD1 A:TYR198 4.5 26.4 1.0 HA A:ASN116 4.6 25.1 1.0 N A:TYR198 4.6 23.0 1.0 HB3 A:TYR198 4.6 26.1 1.0 CA A:ASP154 4.6 22.1 1.0 HB2 A:ASP151 4.6 30.6 1.0 HE2 A:PHE194 4.6 30.7 1.0 O A:LEU163 4.7 27.1 1.0 HB2 A:ASP162 4.7 29.4 1.0 HA A:TYR198 4.7 27.0 1.0 CB A:ASN116 4.7 24.5 1.0 CA A:GLY153 4.7 25.9 1.0 OD1 A:ASP162 4.7 28.4 1.0 NH1 A:ARG137 4.7 26.1 1.0 H A:ASP154 4.7 28.5 1.0 HB3 A:ASP151 4.8 30.6 1.0 N A:GLY153 4.8 25.4 1.0 HH12 A:ARG137 4.9 31.4 1.0 HB2 A:ARG137 4.9 33.0 1.0 HB3 A:ARG155 4.9 29.4 1.0 HA A:ASP162 5.0 29.4 1.0 HB2 A:ASN116 5.0 29.4 1.0

Z.Q.Zhang, X.F.Ban, Z.F.Li, T.C.Jin, Y.L.Li, Z.B.Gu, C.M.Li, Y.H.Shang. Maltotetraose-Forming Amylase From Pseudomonas Saccharophila STB07 To Be Published.