Calcium in PDB 6j3x: The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose

All present enzymatic activity of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose:
3.2.1.60;

The structure of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose, PDB code: 6j3x was solved by Z.F.Li, X.F.Ban, Z.Q.Zhang, C.M.Li, Z.B.Gu, T.C.Jin, Y.L.Li, Y.H.Shang, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	42.47 / 1.62
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	46.320, 64.650, 169.010, 90.00, 90.00, 90.00
R / Rfree (%)	16.4 / 18.6

The binding sites of Calcium atom in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose (pdb code 6j3x). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose, PDB code: 6j3x:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca601 b:28.1 occ:1.00 OD1 A:ASP1 2.2 42.3 1.0 OE1 A:GLU17 2.2 33.9 1.0 O A:HIS13 2.3 27.8 1.0 O A:GLN2 2.3 31.9 1.0 OD1 A:ASP16 2.3 29.1 1.0 ND1 A:HIS13 2.6 30.2 1.0 HZ2 A:LYS108 3.3 52.1 1.0 CG A:ASP1 3.4 43.1 1.0 CG A:ASP16 3.4 33.6 1.0 CD A:GLU17 3.4 34.0 1.0 C A:HIS13 3.4 29.4 1.0 CE1 A:HIS13 3.4 36.4 1.0 HE1 A:HIS13 3.5 43.6 1.0 C A:GLN2 3.5 39.3 1.0 HB3 A:HIS13 3.5 39.0 1.0 HA3 A:GLY14 3.6 34.8 1.0 HG2 A:GLU17 3.6 33.8 1.0 CG A:HIS13 3.6 31.7 1.0 OD2 A:ASP16 3.7 36.8 1.0 HE3 A:LYS108 3.8 51.0 1.0 HA A:ALA3 3.8 41.3 1.0 H A:ASP16 3.8 32.2 1.0 OD2 A:ASP1 3.9 50.2 1.0 CB A:HIS13 4.0 32.5 1.0 H A:GLN2 4.0 48.8 1.0 N A:GLN2 4.0 40.7 1.0 CG A:GLU17 4.1 28.2 1.0 NZ A:LYS108 4.1 43.4 1.0 N A:GLY14 4.2 27.1 1.0 CA A:GLY14 4.3 29.0 1.0 HA A:ASP1 4.3 55.5 1.0 CA A:HIS13 4.3 32.7 1.0 CA A:GLN2 4.3 44.4 1.0 OE2 A:GLU17 4.4 33.8 1.0 C A:ASP1 4.4 44.9 1.0 CE A:LYS108 4.4 42.5 1.0 N A:ALA3 4.4 38.0 1.0 HZ3 A:LYS108 4.4 52.1 1.0 HB2 A:GLN2 4.5 56.1 1.0 CA A:ALA3 4.6 34.4 1.0 CB A:ASP1 4.6 41.3 1.0 HG3 A:GLU17 4.6 33.8 1.0 NE2 A:HIS13 4.6 33.0 1.0 N A:ASP16 4.6 26.8 1.0 CA A:ASP1 4.7 46.2 1.0 CD2 A:HIS13 4.7 32.8 1.0 CB A:ASP16 4.7 28.0 1.0 HZ1 A:LYS108 4.7 52.1 1.0 O A:HOH885 4.8 44.8 1.0 HE2 A:LYS108 4.8 51.0 1.0 HA A:HIS13 4.8 39.2 1.0 H A:GLY15 4.8 33.4 1.0 C A:ASP16 4.8 27.4 1.0 C A:GLY14 4.8 28.4 1.0 N A:GLU17 4.8 25.0 1.0 HB2 A:HIS13 4.9 39.0 1.0 HA A:GLU17 4.9 29.8 1.0 H A:GLU17 4.9 30.0 1.0 CB A:GLN2 4.9 46.8 1.0 CA A:ASP16 5.0 27.2 1.0 O A:ASP1 5.0 49.8 1.0 N A:GLY15 5.0 27.9 1.0

Calcium binding site 2 out of 2 in the The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Structure of Maltooligosaccharide-Forming Amylase From Pseudomonas Saccharophila STB07 with Maltotriose within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca602 b:25.8 occ:1.00 OD1 A:ASN116 2.3 26.0 1.0 O A:GLY197 2.3 24.9 1.0 O A:ASP154 2.4 25.7 1.0 OD2 A:ASP151 2.4 25.8 1.0 O A:HOH718 2.4 25.4 1.0 OD2 A:ASP162 2.6 26.8 1.0 OD1 A:ASP151 2.6 27.1 1.0 CG A:ASP151 2.8 28.4 1.0 HA A:ARG155 3.3 36.0 1.0 C A:GLY197 3.4 25.6 1.0 CG A:ASN116 3.4 23.4 1.0 HB3 A:ASP162 3.5 32.4 1.0 HA2 A:GLY197 3.5 31.3 1.0 HA3 A:GLY197 3.5 31.3 1.0 HD21 A:ASN116 3.6 30.1 1.0 C A:ASP154 3.6 26.6 1.0 CG A:ASP162 3.7 26.6 1.0 CA A:GLY197 3.7 26.1 1.0 ND2 A:ASN116 3.9 25.1 1.0 CB A:ASP162 4.1 27.0 1.0 HH11 A:ARG137 4.1 34.1 1.0 H A:GLY153 4.2 37.5 1.0 CA A:ARG155 4.2 30.0 1.0 HD2 A:ARG137 4.2 38.0 1.0 O A:GLY153 4.2 29.2 1.0 C A:GLY153 4.3 31.7 1.0 CB A:ASP151 4.3 29.5 1.0 N A:ARG155 4.4 27.8 1.0 O A:ASN116 4.4 26.2 1.0 N A:ASP154 4.4 26.6 1.0 O A:HOH792 4.4 28.3 1.0 HA A:ASN116 4.5 27.1 1.0 HD1 A:TYR198 4.5 31.0 1.0 HB3 A:TYR198 4.5 31.0 1.0 HA3 A:GLY153 4.6 34.2 1.0 N A:TYR198 4.6 24.8 1.0 HB2 A:ASP151 4.6 35.4 1.0 CA A:ASP154 4.6 27.3 1.0 CB A:ASN116 4.7 25.6 1.0 O A:LEU163 4.7 28.8 1.0 HE2 A:PHE194 4.7 32.6 1.0 HB2 A:ASP162 4.7 32.4 1.0 HD22 A:ASN116 4.7 30.1 1.0 HA A:TYR198 4.7 30.1 1.0 HB3 A:ASP151 4.8 35.4 1.0 OD1 A:ASP162 4.8 31.9 1.0 H A:ASP154 4.8 31.9 1.0 NH1 A:ARG137 4.8 28.4 1.0 CA A:GLY153 4.8 28.5 1.0 N A:GLY153 4.9 31.3 1.0 HB2 A:ASN116 4.9 30.8 1.0 HB2 A:ARG137 4.9 35.4 1.0 H A:PHE156 4.9 32.1 1.0 HB3 A:ARG155 4.9 37.0 1.0 HA A:ASP162 5.0 33.1 1.0 CA A:ASN116 5.0 22.6 1.0 HH12 A:ARG137 5.0 34.1 1.0

Z.Q.Zhang, X.F.Ban, Z.F.Li, T.C.Jin, Y.L.Li, Z.B.Gu, C.M.Li, Y.H.Shang. Maltotetraose-Forming Amylase From Pseudomonas Saccharophila STB07 To Be Published.