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Calcium in PDB 7aq7: Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y

Enzymatic activity of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y

All present enzymatic activity of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y:
1.7.2.4;

Protein crystallography data

The structure of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y, PDB code: 7aq7 was solved by L.Zhang, E.Bill, P.M.H.Kroneck, O.Einsle, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 68.61 / 1.61
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 68.734, 76.071, 108.467, 90, 93.49, 90
R / Rfree (%) 15.2 / 20.4

Other elements in 7aq7:

The structure of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y also contains other interesting chemical elements:

Chlorine (Cl) 3 atoms
Sodium (Na) 2 atoms
Copper (Cu) 12 atoms
Potassium (K) 2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y (pdb code 7aq7). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y, PDB code: 7aq7:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 7aq7

Go back to Calcium Binding Sites List in 7aq7
Calcium binding site 1 out of 2 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca703

b:20.6
occ:0.38
OE2 A:GLU259 2.3 85.0 1.0
OD1 A:ASN324 2.3 40.7 1.0
OD2 A:ASP273 2.4 42.2 1.0
O A:MET267 2.4 57.9 1.0
O A:TYR256 2.4 45.1 1.0
OD1 A:ASP273 2.6 42.4 1.0
OE1 A:GLU259 2.7 87.4 1.0
CD A:GLU259 2.8 87.9 1.0
CG A:ASP273 2.8 40.3 1.0
O A:HOH812 2.9 47.8 1.0
CG A:ASN324 3.4 34.6 1.0
C A:MET267 3.4 60.6 1.0
C A:TYR256 3.5 42.8 1.0
ND2 A:ASN324 4.0 33.6 1.0
CG A:GLU259 4.2 89.0 1.0
N A:MET268 4.3 61.6 1.0
CA A:ASN257 4.3 52.4 1.0
CA A:MET267 4.3 61.8 1.0
N A:ASN257 4.3 41.5 1.0
CA A:MET268 4.3 62.8 1.0
N A:SER258 4.3 41.8 1.0
OG A:SER258 4.4 54.6 1.0
CA A:TYR256 4.4 45.7 1.0
CB A:ASP273 4.4 26.1 1.0
N A:ASN324 4.5 27.6 1.0
CA A:ASN324 4.5 26.6 1.0
CB A:ASN324 4.6 29.6 1.0
CB A:ASN270 4.7 56.1 1.0
CB A:MET267 4.7 62.0 1.0
C A:MET268 4.7 70.2 1.0
C A:ASN257 4.8 51.3 1.0
O A:ASN270 4.8 55.0 1.0
N A:ASN270 4.8 65.4 1.0
N A:ARG269 4.9 75.0 1.0
CB A:TYR256 5.0 45.5 1.0

Calcium binding site 2 out of 2 in 7aq7

Go back to Calcium Binding Sites List in 7aq7
Calcium binding site 2 out of 2 in the Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Pseudomonas Stutzeri Nitrous Oxide Reductase Mutant, H583Y within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca703

b:26.2
occ:0.71
OD1 B:ASN324 2.3 28.8 1.0
O B:HOH820 2.3 35.8 1.0
O B:MET267 2.4 40.5 1.0
O B:TYR256 2.4 34.6 1.0
OE2 B:GLU259 2.5 40.7 1.0
OD2 B:ASP273 2.5 35.4 1.0
OD1 B:ASP273 2.7 32.9 1.0
OE1 B:GLU259 2.8 36.4 1.0
CG B:ASP273 2.9 30.3 1.0
CD B:GLU259 3.0 40.6 1.0
CG B:ASN324 3.3 32.3 1.0
C B:MET267 3.5 42.6 1.0
C B:TYR256 3.5 37.1 1.0
ND2 B:ASN324 3.8 28.4 1.0
OG B:SER258 4.3 40.5 1.0
N B:MET268 4.3 36.8 1.0
CA B:MET268 4.3 43.7 1.0
CA B:TYR256 4.4 35.2 1.0
N B:ASN257 4.4 38.9 1.0
N B:SER258 4.4 36.0 1.0
CA B:MET267 4.4 40.5 1.0
CB B:ASP273 4.5 28.2 1.0
CA B:ASN257 4.5 38.8 1.0
N B:ASN324 4.5 33.6 1.0
CG B:GLU259 4.5 38.7 1.0
O B:ASN270 4.5 38.4 1.0
CB B:ASN324 4.5 28.2 1.0
CA B:ASN324 4.6 25.8 1.0
C B:MET268 4.6 40.4 1.0
N B:ARG269 4.8 44.9 1.0
N B:ASN270 4.8 53.5 1.0
CB B:ASN270 4.9 45.0 1.0
C B:ASN257 5.0 42.5 1.0
CB B:TYR256 5.0 29.1 1.0

Reference:

L.Zhang, E.Bill, P.M.H.Kroneck, O.Einsle. Histidine-Gated Proton-Coupled Electron Transfer to the Cu A Site of Nitrous Oxide Reductase. J.Am.Chem.Soc. 2020.
ISSN: ESSN 1520-5126
PubMed: 33377777
DOI: 10.1021/JACS.0C10057
Page generated: Thu Jul 18 23:05:22 2024

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