Calcium in PDB 7ey3: Double Cysteine Mutations in T1 Lipase

All present enzymatic activity of Double Cysteine Mutations in T1 Lipase:
3.1.1.3;

The structure of Double Cysteine Mutations in T1 Lipase, PDB code: 7ey3 was solved by S.H.Hamdan, T.C.Leow, N.M.Yahaya, M.S.M.Ali, M.A.Jonet, S.N.A.Mohamadaris, J.Maiangwa, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	33.46 / 2.04
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	118.174, 81.496, 100.046, 90, 96.94, 90
R / Rfree (%)	18.8 / 22.2

The structure of Double Cysteine Mutations in T1 Lipase also contains other interesting chemical elements:

Sodium	(Na)	2 atoms
Zinc	(Zn)	2 atoms
Chlorine	(Cl)	2 atoms

The binding sites of Calcium atom in the Double Cysteine Mutations in T1 Lipase (pdb code 7ey3). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Double Cysteine Mutations in T1 Lipase, PDB code: 7ey3:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Double Cysteine Mutations in T1 Lipase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Double Cysteine Mutations in T1 Lipase within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca2002 b:34.2 occ:1.00 O A:GLY286 2.3 17.3 1.0 OE2 A:GLU360 2.3 13.5 1.0 O A:PRO366 2.4 15.4 1.0 OD2 A:ASP365 2.4 16.9 1.0 O A:HOH2259 2.6 21.4 1.0 CD A:GLU360 3.2 16.4 1.0 O A:HOH2227 3.4 25.4 1.0 CG A:GLU360 3.4 16.5 1.0 C A:GLY286 3.5 17.2 1.0 CG A:ASP365 3.6 17.6 1.0 C A:PRO366 3.6 16.7 1.0 N A:ASN288 4.1 11.1 1.0 NH2 A:ARG271 4.2 15.8 1.0 C A:MET287 4.3 14.9 1.0 CA A:GLY286 4.3 21.6 1.0 CB A:ASN367 4.3 13.8 1.0 CB A:ASP365 4.4 12.7 1.0 OE1 A:GLU360 4.4 14.4 1.0 CA A:MET287 4.4 14.7 1.0 N A:MET287 4.4 16.4 1.0 N A:PRO366 4.5 14.6 1.0 N A:ASN367 4.5 14.9 1.0 CA A:ASN367 4.5 16.2 1.0 OD1 A:ASP365 4.5 19.2 1.0 C A:ASP365 4.6 14.4 1.0 OD2 A:ASP357 4.6 17.4 1.0 CA A:PRO366 4.6 15.4 1.0 CA A:ASN288 4.6 16.7 1.0 O A:HOH2122 4.7 21.5 1.0 O A:MET287 4.8 14.1 1.0 CD A:PRO366 4.8 14.2 1.0 CG A:PRO366 4.8 16.3 1.0 O A:ASP365 4.9 14.1 1.0 CB A:GLU360 4.9 12.8 1.0

Calcium binding site 2 out of 2 in the Double Cysteine Mutations in T1 Lipase


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Double Cysteine Mutations in T1 Lipase within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca402 b:38.2 occ:1.00 O B:GLY286 2.3 14.0 1.0 OD2 B:ASP365 2.3 21.9 1.0 OE2 B:GLU360 2.5 20.3 1.0 O B:PRO366 2.5 17.3 1.0 CD B:GLU360 3.4 16.1 1.0 C B:GLY286 3.5 17.2 1.0 CG B:ASP365 3.5 19.5 1.0 CG B:GLU360 3.6 16.0 1.0 C B:PRO366 3.7 22.5 1.0 N B:ASN288 4.2 14.8 1.0 NH2 B:ARG271 4.2 16.2 1.0 C B:MET287 4.3 17.6 1.0 CA B:GLY286 4.3 18.9 1.0 OD1 B:ASP365 4.3 19.5 1.0 N B:PRO366 4.4 18.3 1.0 CB B:ASP365 4.4 18.9 1.0 N B:MET287 4.4 16.7 1.0 CA B:MET287 4.5 17.3 1.0 CB B:ASN367 4.5 21.9 1.0 O B:HOH537 4.5 24.4 1.0 CG B:PRO366 4.6 20.6 1.0 OE1 B:GLU360 4.6 15.7 1.0 CA B:PRO366 4.6 17.6 1.0 C B:ASP365 4.6 19.6 1.0 N B:ASN367 4.6 15.9 1.0 CA B:ASN367 4.6 22.2 1.0 CA B:ASN288 4.7 17.1 1.0 CD B:PRO366 4.7 19.8 1.0 O B:MET287 4.7 15.8 1.0 O B:ASP365 4.9 17.3 1.0 OD2 B:ASP357 5.0 22.3 1.0

S.H.Hamdan, T.C.Leow. Double Cysteine Mutations in T1 Lipase To Be Published.