Calcium in PDB 8em0: Hras R97V Crystal Form 1

All present enzymatic activity of Hras R97V Crystal Form 1:
3.6.5.2;

The structure of Hras R97V Crystal Form 1, PDB code: 8em0 was solved by C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.03 / 2.11
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	89.146, 89.146, 135.545, 90, 90, 120
R / Rfree (%)	15.6 / 20.1

The structure of Hras R97V Crystal Form 1 also contains other interesting chemical elements:

Magnesium

(Mg)

2 atoms

The binding sites of Calcium atom in the Hras R97V Crystal Form 1 (pdb code 8em0). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Hras R97V Crystal Form 1, PDB code: 8em0:

Calcium binding site 1 out of 1 in the Hras R97V Crystal Form 1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Hras R97V Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca202 b:28.5 occ:1.00 O A:PHE28 2.3 22.8 1.0 O A:HOH414 2.4 31.1 1.0 OD1 A:ASP30 2.4 33.3 1.0 C A:PHE28 3.6 30.3 1.0 CG A:ASP30 3.6 31.5 1.0 N A:ASP30 4.0 22.8 1.0 O A:HOH410 4.2 49.8 1.0 CA A:VAL29 4.3 26.3 1.0 N A:VAL29 4.4 27.0 1.0 CB A:ASP30 4.5 34.1 1.0 OD2 A:ASP30 4.5 37.7 1.0 CA A:PHE28 4.6 25.4 1.0 O A:HOH373 4.6 33.1 1.0 C A:VAL29 4.6 27.5 1.0 N A:PHE28 4.7 25.7 1.0 CB A:PHE28 4.8 25.0 1.0 ND1 A:HIS27 4.9 28.5 1.0 CA A:ASP30 4.9 30.0 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767