Calcium in PDB 8s5a: The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna

All present enzymatic activity of The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna:
3.1.4.1;

The structure of The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna, PDB code: 8s5a was solved by E.Costanzi, M.Thomsen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.40 / 2.65
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	91.626, 100.547, 112.879, 90, 90, 90
R / Rfree (%)	23.4 / 27.3

The structure of The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna also contains other interesting chemical elements:

Chlorine

(Cl)

1 atom

The binding sites of Calcium atom in the The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna (pdb code 8s5a). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna, PDB code: 8s5a:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1101 b:100.3 occ:1.00 OD2 A:ASP960 2.3 77.9 1.0 O A:VAL976 2.3 90.0 1.0 OE2 A:GLU975 2.6 94.2 1.0 CG A:ASP960 3.1 75.2 1.0 OD1 A:ASP960 3.2 79.7 1.0 C A:VAL976 3.5 86.3 1.0 CD A:GLU975 3.6 89.9 1.0 OE2 A:GLU815 3.8 102.0 1.0 OE1 A:GLU975 4.2 92.0 1.0 N A:VAL976 4.3 81.6 1.0 CA A:VAL976 4.3 81.5 1.0 CD A:LYS977 4.4 98.3 1.0 CB A:VAL976 4.5 83.1 1.0 N A:LYS977 4.5 89.8 1.0 CB A:ASP960 4.5 71.7 1.0 CG A:GLU975 4.5 91.3 1.0 OD2 A:ASP981 4.7 117.5 1.0 CA A:LYS977 4.7 92.9 1.0 NZ A:LYS977 4.7 97.6 1.0 CD A:GLU815 4.9 104.6 1.0 CE A:LYS977 4.9 98.8 1.0 C A:GLU975 5.0 86.0 1.0

Calcium binding site 2 out of 2 in the The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1102 b:109.1 occ:1.00 O A:PRO959 2.7 65.1 1.0 O A:GLU834 3.2 97.8 1.0 CG2 A:VAL962 3.5 92.0 1.0 C A:PRO959 3.9 68.9 1.0 CD2 A:PHE838 4.1 87.3 1.0 CE2 A:PHE838 4.1 88.7 1.0 O A:LEU961 4.1 84.9 1.0 C A:GLU834 4.2 89.4 1.0 CG A:PRO959 4.2 72.1 1.0 C A:LEU961 4.3 82.2 1.0 O A:ASP960 4.3 74.5 1.0 CB A:THR837 4.3 68.7 1.0 CD A:PRO959 4.4 72.2 1.0 C A:ASP960 4.4 69.9 1.0 N A:PRO959 4.4 70.7 1.0 N A:VAL962 4.5 81.1 1.0 OG1 A:THR837 4.5 69.5 1.0 CA A:GLU834 4.6 86.9 1.0 N A:LEU961 4.6 74.2 1.0 CA A:PRO959 4.6 71.1 1.0 N A:GLU834 4.6 80.4 1.0 CB A:PRO959 4.8 71.7 1.0 CB A:VAL962 4.9 83.3 1.0 N A:ASP960 4.9 68.5 1.0 CA A:LEU961 4.9 80.6 1.0 CG2 A:THR837 4.9 67.4 1.0 C A:LEU958 4.9 71.6 1.0 CB A:LEU958 5.0 72.7 1.0

E.Costanzi, M.Thomsen. The Crystal Structure of FAN1 Nuclease Bound to 5' Phosphorylated P(Dg)/3'(Dt-Dt-Dt-Dt) Double Flap Dna To Be Published.