Calcium in PDB 9ga6: The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2

The structure of The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2, PDB code: 9ga6 was solved by L.Vitagliano, G.Barra, O.Ghilardi, S.Di Micco, G.Bifulco, P.Campiglia, M.Sala, M.C.Scala, A.Ruggiero, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	58.60 / 1.27
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	58.742, 39.906, 73.456, 90, 93.91, 90
R / Rfree (%)	17.4 / 18.9

The binding sites of Calcium atom in the The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2 (pdb code 9ga6). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 3 binding sites of Calcium where determined in the The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2, PDB code: 9ga6:
Jump to Calcium binding site number: 1; 2; 3;

Calcium binding site 1 out of 3 in the The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:11.3 occ:1.00 O A:MET27 2.3 10.2 1.0 O A:GLY29 2.3 13.4 1.0 O A:HOH747 2.4 17.1 1.0 O A:GLY31 2.4 11.6 1.0 O A:HOH699 2.4 18.9 1.0 OE2 A:GLU71 2.5 13.5 1.0 OE1 A:GLU71 2.5 12.2 1.0 CD A:GLU71 2.8 12.0 1.0 C A:MET27 3.5 9.9 1.0 C A:GLY31 3.5 11.2 1.0 C A:GLY29 3.5 13.2 1.0 N A:GLY31 3.8 12.6 1.0 N A:GLY29 4.1 12.7 1.0 C A:LEU30 4.1 12.9 1.0 CA A:GLY31 4.2 12.6 1.0 CA A:MET27 4.2 8.8 1.0 C A:LYS28 4.2 11.8 1.0 OG1 A:THR32 4.3 12.2 1.0 CG A:GLU71 4.3 12.0 1.0 CA A:GLY29 4.5 13.4 1.0 O A:HOH610 4.5 18.5 1.0 CA A:LEU30 4.5 16.3 1.0 N A:LEU30 4.5 14.0 1.0 N A:LYS28 4.5 9.7 1.0 N A:THR32 4.6 10.4 1.0 O A:LYS28 4.6 11.7 1.0 O A:HOH559 4.7 30.3 1.0 O A:LEU30 4.7 12.8 1.0 CA A:LYS28 4.7 10.6 1.0 CB A:MET27 4.8 8.5 1.0 CA A:THR32 4.9 9.1 1.0 O A:HOH672 4.9 29.1 1.0

Calcium binding site 2 out of 3 in the The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:28.3 occ:1.00 O A:MET99 2.8 10.6 1.0 O A:GLY101 2.8 14.2 1.0 O A:GLY103 2.9 13.7 1.0 OG1 A:THR104 3.0 14.0 1.0 OD2 A:ASP143 3.1 13.1 1.0 O A:ALA102 3.5 22.0 1.0 CG A:ASP143 3.5 11.4 1.0 C A:GLY103 3.6 13.7 1.0 C A:MET99 3.7 9.5 1.0 OD1 A:ASP143 3.9 12.9 1.0 C A:ALA102 3.9 18.2 1.0 C A:GLY101 3.9 12.5 1.0 O A:HOH525 4.1 14.2 1.0 CA A:MET99 4.1 9.3 1.0 O A:HOH555 4.2 14.5 1.0 CB A:THR104 4.2 12.3 1.0 N A:THR104 4.3 13.1 1.0 N A:GLY103 4.3 18.0 1.0 CB A:ASP143 4.4 9.9 1.0 CA A:THR104 4.4 11.9 1.0 CA A:GLY103 4.5 16.6 1.0 O A:LYS100 4.5 12.8 1.0 C A:LYS100 4.5 11.7 1.0 CA A:ALA102 4.6 14.2 1.0 O A:HOH609 4.6 18.6 1.0 N A:ALA102 4.6 12.9 1.0 N A:LYS100 4.7 10.0 1.0 N A:GLY101 4.7 11.6 1.0 CB A:MET99 4.8 9.4 1.0 CG2 A:THR104 4.9 12.1 1.0 CA A:GLY101 4.9 13.2 1.0

Calcium binding site 3 out of 3 in the The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of The Crystal Structure of Human Annexin A4 Derived From Crystals Grown in 40 Mm of CACL2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:24.2 occ:1.00 O A:MET258 2.4 13.8 1.0 O A:HOH727 2.4 24.9 1.0 O A:GLY260 2.4 27.4 1.0 O A:GLY262 2.4 25.5 1.0 O A:HOH615 2.6 47.3 1.0 OD1 A:ASP302 2.7 26.2 1.0 OD2 A:ASP302 2.8 27.2 1.0 CG A:ASP302 3.0 21.4 1.0 C A:MET258 3.5 11.4 1.0 C A:GLY262 3.5 24.1 1.0 C A:GLY260 3.6 27.6 1.0 N A:GLY262 3.7 28.8 1.0 OG1 A:THR263 4.1 20.7 1.0 CA A:GLY262 4.1 26.5 1.0 CA A:MET258 4.1 10.8 1.0 C A:LEU261 4.2 29.7 1.0 C A:LYS259 4.2 18.9 1.0 O A:LYS259 4.3 23.0 1.0 N A:GLY260 4.3 20.6 1.0 N A:LYS259 4.5 13.2 1.0 CA A:GLY260 4.5 27.0 1.0 N A:LEU261 4.5 29.0 1.0 CB A:ASP302 4.6 17.2 1.0 CA A:LEU261 4.6 32.5 1.0 N A:THR263 4.6 16.4 1.0 CA A:LYS259 4.7 16.1 1.0 O A:HOH663 4.7 27.9 1.0 CB A:MET258 4.8 10.7 1.0 CA A:THR263 4.8 15.3 1.0 O A:LEU261 4.8 31.9 1.0

S.Di Micco, M.C.Scala, M.Sala, G.Barra, O.Ghilardi, P.Campiglia, G.Bifulco, L.Vitagliano, A.Ruggiero. Computational, Crystallographic, and Biophysical Characterizations Provide Insights Into Calcium and Phosphate Binding By Human Annexin A4. Int.J.Biol.Macromol. V. 308 42600 2025.
ISSN: ISSN 0141-8130
PubMed: 40157693
DOI: 10.1016/J.IJBIOMAC.2025.142600