Calcium in PDB 1w36: Recbcd:Dna Complex

All present enzymatic activity of Recbcd:Dna Complex:
3.1.11.5;

The structure of Recbcd:Dna Complex, PDB code: 1w36 was solved by M.R.Singleton, M.S.Dillingham, M.Gaudier, S.C Kowalczykowski, D.B.Wigley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.84 / 3.1
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	132.602, 187.234, 335.369, 90.00, 90.00, 90.00
R / Rfree (%)	24.2 / 29.6

The binding sites of Calcium atom in the Recbcd:Dna Complex (pdb code 1w36). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Recbcd:Dna Complex, PDB code: 1w36:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Recbcd:Dna Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Recbcd:Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca4000 b:67.8 occ:1.00 OD2 B:ASP1067 2.2 70.2 1.0 O B:TYR1081 2.4 78.9 1.0 OD1 B:ASP1080 2.5 72.9 1.0 NE2 B:HIS956 2.6 70.1 1.0 CG B:ASP1067 3.3 71.0 1.0 CE1 B:HIS956 3.4 70.0 1.0 CG B:ASP1080 3.5 73.2 1.0 C B:TYR1081 3.6 79.4 1.0 OD1 B:ASP1067 3.6 69.8 1.0 CD2 B:HIS956 3.6 70.1 1.0 OD2 B:ASP1080 3.9 71.6 1.0 NZ B:LYS1082 3.9 72.9 1.0 N B:TYR1081 4.1 78.0 1.0 OD2 B:ASP927 4.1 0.1 1.0 CA B:TYR1081 4.5 78.6 1.0 N B:LYS1082 4.5 80.2 1.0 CG B:LYS1082 4.5 78.8 1.0 C B:ASP1080 4.6 76.6 1.0 CA B:LYS1082 4.6 81.2 1.0 ND1 B:HIS956 4.6 70.5 1.0 CB B:ASP1067 4.6 72.5 1.0 CG B:HIS956 4.7 71.1 1.0 CB B:ASP1080 4.7 74.1 1.0 CE B:LYS1082 4.7 74.4 1.0 CA B:ASP1080 4.8 75.2 1.0 CB B:TYR1081 5.0 77.8 1.0

Calcium binding site 2 out of 2 in the Recbcd:Dna Complex


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Recbcd:Dna Complex within 5.0Å range: probe atom residue distance (Å) B Occ E:Ca4000 b:52.0 occ:1.00 OD2 E:ASP1067 2.2 52.5 1.0 OD1 E:ASP1080 2.5 62.4 1.0 O E:TYR1081 2.6 55.2 1.0 NE2 E:HIS956 2.6 41.7 1.0 CG E:ASP1067 3.1 54.5 1.0 OD1 E:ASP1067 3.4 57.7 1.0 CG E:ASP1080 3.5 61.7 1.0 CE1 E:HIS956 3.5 43.1 1.0 CD2 E:HIS956 3.6 43.0 1.0 C E:TYR1081 3.8 56.1 1.0 OD2 E:ASP1080 3.8 64.1 1.0 NZ E:LYS1082 4.0 45.2 1.0 N E:TYR1081 4.2 59.0 1.0 OD2 E:ASP927 4.2 93.4 1.0 CB E:ASP1067 4.6 52.8 1.0 CA E:TYR1081 4.6 57.5 1.0 C E:ASP1080 4.7 59.6 1.0 ND1 E:HIS956 4.7 43.2 1.0 CB E:ASP1080 4.7 59.5 1.0 CG E:HIS956 4.7 44.1 1.0 CG E:LYS1082 4.8 48.8 1.0 N E:LYS1082 4.8 55.6 1.0 CA E:ASP1080 4.8 58.8 1.0 CA E:LYS1082 4.9 56.9 1.0 CE E:LYS1082 4.9 45.0 1.0

M.R.Singleton, M.S.Dillingham, M.Gaudier, S.C.Kowalczykowski, D.B.Wigley. Crystal Structure of Recbcd Enzyme Reveals A Machine For Processing Dna Breaks Nature V. 432 187 2004.
ISSN: ISSN 0028-0836
PubMed: 15538360
DOI: 10.1038/NATURE02988