Calcium in PDB 2b0d: Ecorv Restriction Endonuclease/Gaattc/CA2+

All present enzymatic activity of Ecorv Restriction Endonuclease/Gaattc/CA2+:
3.1.21.4;

The structure of Ecorv Restriction Endonuclease/Gaattc/CA2+, PDB code: 2b0d was solved by D.A.Hiller, A.M.Rodriguez, J.J.Perona, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 2.00
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	48.000, 48.400, 63.600, 97.20, 109.00, 106.60
R / Rfree (%)	22 / 30.2

The binding sites of Calcium atom in the Ecorv Restriction Endonuclease/Gaattc/CA2+ (pdb code 2b0d). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ecorv Restriction Endonuclease/Gaattc/CA2+, PDB code: 2b0d:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ecorv Restriction Endonuclease/Gaattc/CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ecorv Restriction Endonuclease/Gaattc/CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca246 b:47.8 occ:1.00 O A:HOH526 2.1 29.4 1.0 O A:HOH579 2.2 33.0 1.0 OE2 A:GLU45 2.3 38.2 1.0 OD2 A:ASP74 3.1 22.4 1.0 CD A:GLU45 3.2 40.1 1.0 OE1 A:GLU45 3.6 43.5 1.0 CG A:ASP74 3.6 15.8 1.0 O A:HOH606 3.9 58.9 1.0 CB A:ASP74 4.0 18.4 1.0 CA A:ASP74 4.1 18.5 1.0 OE1 A:GLU65 4.3 32.9 1.0 OD1 A:ASP74 4.4 18.3 1.0 CG A:GLU45 4.4 34.5 1.0 O A:ASP74 4.5 15.6 1.0 C A:ASP74 4.8 19.1 1.0 O A:HOH616 5.0 42.0 1.0 CD A:PRO66 5.0 25.6 1.0

Calcium binding site 2 out of 2 in the Ecorv Restriction Endonuclease/Gaattc/CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ecorv Restriction Endonuclease/Gaattc/CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca502 b:81.0 occ:1.00 OP1 C:DT7 2.2 33.7 1.0 O A:HOH616 2.2 42.0 1.0 OD2 A:ASP90 2.7 22.7 1.0 OD2 A:ASP74 2.7 22.4 1.0 O A:HOH578 3.1 34.7 1.0 OD1 A:ASP90 3.2 29.4 1.0 CG A:ASP90 3.2 21.9 1.0 CG A:ASP74 3.4 15.8 1.0 P C:DT7 3.5 24.0 1.0 N A:ASP74 3.7 16.0 1.0 OD1 A:ASP74 3.8 18.3 1.0 O3' C:DA6 3.8 24.2 1.0 O A:HIS71 4.3 26.7 1.0 CA A:ASP74 4.3 18.5 1.0 C A:PRO73 4.3 14.8 1.0 CB A:ASP74 4.4 18.4 1.0 CA A:PRO73 4.5 14.9 1.0 O5' C:DT7 4.5 30.6 1.0 C5' C:DT7 4.5 28.2 1.0 O A:HOH579 4.5 33.0 1.0 O A:HOH586 4.5 28.7 1.0 CD2 A:TYR72 4.6 23.8 1.0 NZ A:LYS92 4.6 22.4 1.0 CB A:ASP90 4.6 23.2 1.0 OP2 C:DT7 4.6 27.0 1.0 O A:HOH606 4.7 58.9 1.0 CA A:TYR72 4.7 24.8 1.0

D.A.Hiller, A.M.Rodriguez, J.J.Perona. Non-Cognate Enzyme-Dna Complex: Structural and Kinetic Analysis of Ecorv Endonuclease Bound to the Ecori Recognition Site Gaattc J.Mol.Biol. V. 354 121 2005.
ISSN: ISSN 0022-2836
PubMed: 16236314
DOI: 10.1016/J.JMB.2005.09.046