Calcium in PDB 2b0e: Ecorv Restriction Endonuclease/Gaautc/CA2+

All present enzymatic activity of Ecorv Restriction Endonuclease/Gaautc/CA2+:
3.1.21.4;

The structure of Ecorv Restriction Endonuclease/Gaautc/CA2+, PDB code: 2b0e was solved by D.A.Hiller, A.M.Rodriguez, J.J.Perona, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	6.00 / 1.90
Space group	P 1
Cell size a, b, c (Å), α, β, γ (°)	48.100, 48.500, 63.900, 97.00, 109.00, 106.70
R / Rfree (%)	23.4 / 31.4

The binding sites of Calcium atom in the Ecorv Restriction Endonuclease/Gaautc/CA2+ (pdb code 2b0e). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Ecorv Restriction Endonuclease/Gaautc/CA2+, PDB code: 2b0e:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Ecorv Restriction Endonuclease/Gaautc/CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Ecorv Restriction Endonuclease/Gaautc/CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca307 b:33.5 occ:1.00 O A:HOH385 2.2 33.9 1.0 O A:HOH381 2.3 42.2 1.0 O A:HOH386 2.3 24.7 1.0 O A:HOH432 2.4 23.8 1.0 OD2 A:ASP74 2.5 21.2 1.0 OE1 A:GLU45 2.5 41.2 1.0 O A:HOH376 2.7 41.9 1.0 CG A:ASP74 3.4 15.2 1.0 CD A:GLU45 3.6 40.6 1.0 CB A:ASP74 3.9 17.1 1.0 OE2 A:GLU45 4.0 38.7 1.0 CA A:ASP74 4.1 18.8 1.0 OD1 A:ASP74 4.2 13.8 1.0 O A:HOH430 4.5 29.2 1.0 OE1 A:GLU65 4.6 29.1 1.0 O A:HOH387 4.8 39.6 1.0 O A:ASP74 4.8 17.3 1.0 O B:HOH351 4.8 37.3 1.0 C A:ASP74 4.9 19.6 1.0 CG A:PRO66 4.9 32.3 1.0 CG A:GLU45 4.9 35.9 1.0

Calcium binding site 2 out of 2 in the Ecorv Restriction Endonuclease/Gaautc/CA2+


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Ecorv Restriction Endonuclease/Gaautc/CA2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca322 b:30.4 occ:1.00 O A:HOH430 2.1 29.2 1.0 O A:HOH431 2.3 34.2 1.0 OD1 A:ASP74 2.3 13.8 1.0 O A:HOH400 2.3 22.3 1.0 O A:ILE91 2.5 18.1 1.0 CG A:ASP74 3.2 15.2 1.0 OE2 A:GLU45 3.3 38.7 1.0 OD2 A:ASP74 3.4 21.2 1.0 C A:ILE91 3.6 16.9 1.0 N A:ILE91 3.8 16.2 1.0 OD2 A:ASP90 3.9 25.5 1.0 CD A:GLU45 4.0 40.6 1.0 OP1 C:DU7 4.0 29.6 1.0 CA A:ILE91 4.1 16.1 1.0 CG A:ASP90 4.1 21.2 1.0 OD1 A:ASP90 4.1 18.6 1.0 C5' C:DU7 4.2 18.0 1.0 O C:HOH328 4.2 19.2 1.0 O A:HOH387 4.2 39.6 1.0 O C:HOH318 4.3 22.6 1.0 CB A:ILE91 4.3 14.9 1.0 C4' C:DU7 4.4 20.4 1.0 OE1 A:GLU45 4.5 41.2 1.0 C3' C:DU7 4.5 19.5 1.0 O3' C:DU7 4.6 15.3 1.0 CB A:ASP74 4.6 17.1 1.0 O A:HOH432 4.7 23.8 1.0 C A:ASP90 4.7 17.9 1.0 N A:LYS92 4.8 17.2 1.0 CG A:GLU45 4.8 35.9 1.0 NZ A:LYS92 4.9 24.0 1.0 O5' C:DU7 5.0 22.5 1.0

D.A.Hiller, A.M.Rodriguez, J.J.Perona. Non-Cognate Enzyme-Dna Complex: Structural and Kinetic Analysis of Ecorv Endonuclease Bound to the Ecori Recognition Site Gaattc J.Mol.Biol. V. 354 121 2005.
ISSN: ISSN 0022-2836
PubMed: 16236314
DOI: 10.1016/J.JMB.2005.09.046