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Calcium in PDB 2bd9: Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump)

Enzymatic activity of Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump)

All present enzymatic activity of Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump):
3.4.21.36;

Protein crystallography data

The structure of Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump), PDB code: 2bd9 was solved by B.Liu, C.J.Schofield, R.C.Wilmouth, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.87 / 1.90
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 51.380, 57.590, 74.758, 90.00, 90.00, 90.00
R / Rfree (%) 17.2 / 22.6

Calcium Binding Sites:

The binding sites of Calcium atom in the Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump) (pdb code 2bd9). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump), PDB code: 2bd9:

Calcium binding site 1 out of 1 in 2bd9

Go back to Calcium Binding Sites List in 2bd9
Calcium binding site 1 out of 1 in the Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Porcine Pancreatic Elastase Complexed with Beta-Casomorphin-7 and Arg- Phe at pH 5.0 (50 Min Soak) and Immersed in pH 9 Buffer For 28 Seconds (2ND pH Jump) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca260

b:30.1
occ:1.00
 O A:HOH378 2.2 42.7 1.0
O A:ASN72 2.3 33.5 1.0
OE1 A:GLU70 2.3 33.3 1.0
ND2 A:ASN77 2.3 33.1 1.0
O A:GLN75 2.4 31.5 1.0
OE1 A:GLU80 2.4 32.2 1.0
OE2 A:GLU70 2.9 38.9 1.0
CD A:GLU70 3.0 30.4 1.0
C A:ASN72 3.4 33.1 1.0
CD A:GLU80 3.5 31.6 1.0
CG A:ASN77 3.5 36.3 1.0
C A:GLN75 3.6 38.5 1.0
N A:ASN77 3.8 36.0 1.0
CG A:GLU80 3.9 28.1 1.0
CB A:ASN77 4.0 35.6 1.0
N A:ASN72 4.1 32.8 1.0
CA A:ASN72 4.2 32.6 1.0
N A:GLN75 4.3 38.9 1.0
CA A:ASN76 4.3 36.7 1.0
N A:ASN76 4.4 36.7 1.0
N A:LEU73 4.4 30.5 1.0
C A:ASN76 4.4 34.4 1.0
CG A:GLU70 4.4 28.3 1.0
CB A:ASN72 4.5 31.2 1.0
OD1 A:ASN77 4.5 38.3 1.0
O A:HOH357 4.5 38.5 1.0
C A:LEU73 4.5 36.8 1.0
CA A:GLN75 4.5 40.6 1.0
CA A:LEU73 4.5 34.5 1.0
CA A:ASN77 4.6 33.1 1.0
OE2 A:GLU80 4.6 31.8 1.0
N A:HIS71 4.7 30.7 1.0
O A:LEU73 4.7 37.8 1.0
O A:HOH377 4.9 26.9 1.0
N A:ASN74 4.9 35.8 1.0

Reference:

B.Liu, C.J.Schofield, R.C.Wilmouth. Structural Analyses on Intermediates in Serine Protease Catalysis J.Biol.Chem. V. 281 24024 2006.
ISSN: ISSN 0021-9258
PubMed: 16754679
DOI: 10.1074/JBC.M600495200
Page generated: Sat Dec 12 03:32:19 2020

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