Calcium in PDB 2z2y: Crystal Structure of Autoprocessed Form of Tk-Subtilisin
Enzymatic activity of Crystal Structure of Autoprocessed Form of Tk-Subtilisin
All present enzymatic activity of Crystal Structure of Autoprocessed Form of Tk-Subtilisin:
3.4.21.62;
Protein crystallography data
The structure of Crystal Structure of Autoprocessed Form of Tk-Subtilisin, PDB code: 2z2y
was solved by
S.Tanaka,
H.Matsumura,
Y.Koga,
K.Takano,
S.Kanaya,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
|
Resolution Low / High (Å)
|
46.88 /
1.89
|
|
Space group
|
P 1
|
|
Cell size a, b, c (Å), α, β, γ (°)
|
53.908,
65.365,
70.385,
87.59,
67.44,
69.76
|
|
R / Rfree (%)
|
16.3 /
19.8
|
Other elements in 2z2y:
The structure of Crystal Structure of Autoprocessed Form of Tk-Subtilisin also contains other interesting chemical elements:
Calcium Binding Sites:
Pages:
>>> Page 1 <<<
Page 2, Binding sites: 11 -
14;
Binding sites:
The binding sites of Calcium atom in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
(pdb code 2z2y). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 14 binding sites of Calcium where determined in the
Crystal Structure of Autoprocessed Form of Tk-Subtilisin, PDB code: 2z2y:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
10;
Calcium binding site 1 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 1 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1001
b:16.3
occ:1.00
|
O
|
A:ILE168
|
2.3
|
18.4
|
1.0
|
|
O
|
A:VAL170
|
2.3
|
18.3
|
1.0
|
|
OE1
|
A:GLN84
|
2.3
|
13.8
|
1.0
|
|
O
|
A:LEU164
|
2.4
|
17.1
|
1.0
|
|
OD1
|
A:ASP124
|
2.4
|
18.7
|
1.0
|
|
OD1
|
A:ASN166
|
2.5
|
20.6
|
1.0
|
|
OD2
|
A:ASP124
|
2.6
|
18.1
|
1.0
|
|
CG
|
A:ASP124
|
2.9
|
18.2
|
1.0
|
|
CD
|
A:GLN84
|
3.4
|
17.2
|
1.0
|
|
CG
|
A:ASN166
|
3.4
|
20.7
|
1.0
|
|
C
|
A:ILE168
|
3.5
|
19.4
|
1.0
|
|
C
|
A:VAL170
|
3.5
|
18.1
|
1.0
|
|
C
|
A:LEU164
|
3.5
|
16.9
|
1.0
|
|
N
|
A:VAL170
|
3.8
|
19.0
|
1.0
|
|
CG
|
A:GLN84
|
3.8
|
18.4
|
1.0
|
|
N
|
A:ASN166
|
3.9
|
20.3
|
1.0
|
|
ND2
|
A:ASN166
|
4.0
|
17.6
|
1.0
|
|
C
|
A:GLY169
|
4.3
|
18.6
|
1.0
|
|
CA
|
A:VAL170
|
4.3
|
18.2
|
1.0
|
|
N
|
A:ILE168
|
4.3
|
20.8
|
1.0
|
|
N
|
A:LEU164
|
4.3
|
16.6
|
1.0
|
|
CB
|
A:ASP124
|
4.4
|
16.5
|
1.0
|
|
N
|
A:GLY169
|
4.4
|
18.9
|
1.0
|
|
CA
|
A:LEU164
|
4.4
|
16.2
|
1.0
|
|
CA
|
A:GLY169
|
4.4
|
19.0
|
1.0
|
|
CB
|
A:GLN84
|
4.4
|
21.9
|
1.0
|
|
CA
|
A:ILE168
|
4.4
|
19.7
|
1.0
|
|
N
|
A:ASN165
|
4.5
|
17.4
|
1.0
|
|
CG1
|
A:ILE168
|
4.5
|
19.4
|
1.0
|
|
CA
|
A:ASN165
|
4.5
|
18.8
|
1.0
|
|
N
|
A:VAL171
|
4.5
|
17.5
|
1.0
|
|
NE2
|
A:GLN84
|
4.5
|
15.2
|
1.0
|
|
CB
|
A:ASN166
|
4.6
|
20.6
|
1.0
|
|
CA
|
A:ASN166
|
4.6
|
20.9
|
1.0
|
|
CA
|
A:VAL171
|
4.6
|
16.8
|
1.0
|
|
C
|
A:ASN166
|
4.7
|
21.7
|
1.0
|
|
C
|
A:ASN165
|
4.7
|
19.4
|
1.0
|
|
O
|
A:ASN166
|
4.7
|
21.8
|
1.0
|
|
CB
|
A:LEU164
|
4.7
|
17.1
|
1.0
|
|
C
|
A:ALA163
|
4.8
|
15.3
|
1.0
|
|
CB
|
A:VAL171
|
4.9
|
16.5
|
1.0
|
|
Calcium binding site 2 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 2 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1002
b:12.6
occ:1.00
|
OD2
|
A:ASP226
|
2.0
|
20.3
|
1.0
|
|
O
|
A:HOH2130
|
2.0
|
27.8
|
1.0
|
|
O
|
A:LEU205
|
2.0
|
20.0
|
1.0
|
|
O
|
A:HOH2081
|
2.1
|
19.7
|
1.0
|
|
O
|
A:VAL210
|
2.1
|
23.0
|
1.0
|
|
OD1
|
A:ASP208
|
2.1
|
26.7
|
1.0
|
|
CG
|
A:ASP208
|
3.1
|
25.7
|
1.0
|
|
CG
|
A:ASP226
|
3.1
|
19.7
|
1.0
|
|
C
|
A:LEU205
|
3.1
|
19.2
|
1.0
|
|
C
|
A:VAL210
|
3.3
|
22.8
|
1.0
|
|
OD2
|
A:ASP208
|
3.4
|
26.2
|
1.0
|
|
OD1
|
A:ASP226
|
3.6
|
20.1
|
1.0
|
|
N
|
A:VAL210
|
3.8
|
22.1
|
1.0
|
|
N
|
A:ASP226
|
3.9
|
20.6
|
1.0
|
|
CA
|
A:LEU205
|
4.0
|
18.0
|
1.0
|
|
N
|
A:GLY206
|
4.0
|
19.9
|
1.0
|
|
CA
|
A:VAL210
|
4.1
|
22.9
|
1.0
|
|
O
|
A:ALA211
|
4.2
|
22.9
|
1.0
|
|
CA
|
A:GLY206
|
4.2
|
21.0
|
1.0
|
|
N
|
A:ALA211
|
4.2
|
23.3
|
1.0
|
|
O
|
A:ILE204
|
4.2
|
17.4
|
1.0
|
|
CA
|
A:ALA211
|
4.3
|
23.1
|
1.0
|
|
CB
|
A:ASP226
|
4.3
|
19.7
|
1.0
|
|
C
|
A:ALA211
|
4.4
|
23.9
|
1.0
|
|
CA
|
A:ASP226
|
4.4
|
20.0
|
1.0
|
|
CB
|
A:ASP208
|
4.5
|
25.2
|
1.0
|
|
C
|
A:ASP226
|
4.5
|
19.0
|
1.0
|
|
N
|
A:ALA227
|
4.5
|
18.1
|
1.0
|
|
OD1
|
A:ASP225
|
4.5
|
23.1
|
1.0
|
|
C
|
A:GLY206
|
4.5
|
21.8
|
1.0
|
|
N
|
A:GLY209
|
4.6
|
23.5
|
1.0
|
|
CB
|
A:VAL210
|
4.6
|
23.1
|
1.0
|
|
N
|
A:ASP208
|
4.6
|
24.6
|
1.0
|
|
O
|
A:GLY206
|
4.7
|
22.5
|
1.0
|
|
CA
|
A:ASP208
|
4.8
|
25.0
|
1.0
|
|
CB
|
A:ASP225
|
4.9
|
22.2
|
1.0
|
|
C
|
A:ASP208
|
4.9
|
25.1
|
1.0
|
|
C
|
A:ASP225
|
4.9
|
21.8
|
1.0
|
|
CA
|
A:ASP225
|
4.9
|
22.2
|
1.0
|
|
CB
|
A:LEU205
|
4.9
|
18.2
|
1.0
|
|
C
|
A:GLY209
|
4.9
|
22.8
|
1.0
|
|
O
|
A:ASP226
|
5.0
|
18.6
|
1.0
|
|
Calcium binding site 3 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 3 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1003
b:20.0
occ:1.00
|
OD1
|
A:ASP212
|
2.2
|
23.4
|
1.0
|
|
OD1
|
A:ASP222
|
2.2
|
22.2
|
1.0
|
|
O
|
A:ILE218
|
2.2
|
23.9
|
1.0
|
|
OD2
|
A:ASP225
|
2.3
|
23.6
|
1.0
|
|
OD2
|
A:ASP214
|
2.4
|
31.4
|
1.0
|
|
OD1
|
A:ASP216
|
2.5
|
23.2
|
1.0
|
|
CG
|
A:ASP222
|
3.3
|
21.7
|
1.0
|
|
CG
|
A:ASP216
|
3.4
|
25.9
|
1.0
|
|
CG
|
A:ASP212
|
3.4
|
25.0
|
1.0
|
|
C
|
A:ILE218
|
3.4
|
24.5
|
1.0
|
|
CG
|
A:ASP225
|
3.5
|
22.2
|
1.0
|
|
CG
|
A:ASP214
|
3.5
|
32.0
|
1.0
|
|
CA
|
A:CA1004
|
3.6
|
17.1
|
1.0
|
|
OD2
|
A:ASP216
|
3.6
|
23.6
|
1.0
|
|
OD1
|
A:ASP214
|
3.9
|
33.2
|
1.0
|
|
CB
|
A:ASP222
|
3.9
|
21.3
|
1.0
|
|
N
|
A:ILE218
|
4.0
|
25.9
|
1.0
|
|
OD1
|
A:ASP225
|
4.1
|
23.1
|
1.0
|
|
CA
|
A:ILE218
|
4.1
|
25.4
|
1.0
|
|
OD2
|
A:ASP222
|
4.2
|
21.6
|
1.0
|
|
OD2
|
A:ASP212
|
4.2
|
25.9
|
1.0
|
|
CA
|
A:ASP212
|
4.3
|
24.7
|
1.0
|
|
N
|
A:ASP216
|
4.3
|
28.1
|
1.0
|
|
CB
|
A:ILE218
|
4.3
|
25.4
|
1.0
|
|
CB
|
A:ASP212
|
4.4
|
23.9
|
1.0
|
|
N
|
A:ASP214
|
4.4
|
30.3
|
1.0
|
|
N
|
A:ILE219
|
4.5
|
23.9
|
1.0
|
|
C
|
A:ASP212
|
4.5
|
25.9
|
1.0
|
|
CB
|
A:ASP225
|
4.6
|
22.2
|
1.0
|
|
N
|
A:GLY215
|
4.6
|
30.9
|
1.0
|
|
N
|
A:LYS213
|
4.7
|
27.1
|
1.0
|
|
CB
|
A:ASP216
|
4.7
|
27.3
|
1.0
|
|
CA
|
A:ILE219
|
4.7
|
22.4
|
1.0
|
|
N
|
A:GLY217
|
4.7
|
26.7
|
1.0
|
|
CB
|
A:ASP214
|
4.8
|
31.1
|
1.0
|
|
CA
|
A:ASP216
|
4.9
|
27.2
|
1.0
|
|
CA
|
A:ASP214
|
4.9
|
31.2
|
1.0
|
|
C
|
A:ASP214
|
4.9
|
31.0
|
1.0
|
|
C
|
A:GLY217
|
5.0
|
26.5
|
1.0
|
|
Calcium binding site 4 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 4 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1004
b:17.1
occ:1.00
|
OD2
|
A:ASP216
|
1.8
|
23.6
|
1.0
|
|
OD1
|
A:ASP214
|
2.0
|
33.2
|
1.0
|
|
OD1
|
A:ASP222
|
2.2
|
22.2
|
1.0
|
|
CG
|
A:ASP222
|
2.8
|
21.7
|
1.0
|
|
OD2
|
A:ASP222
|
2.8
|
21.6
|
1.0
|
|
CG
|
A:ASP214
|
2.9
|
32.0
|
1.0
|
|
CG
|
A:ASP216
|
2.9
|
25.9
|
1.0
|
|
OD2
|
A:ASP214
|
3.1
|
31.4
|
1.0
|
|
OD1
|
A:ASP216
|
3.4
|
23.2
|
1.0
|
|
CA
|
A:CA1003
|
3.6
|
20.0
|
1.0
|
|
O
|
A:HOH2250
|
4.1
|
40.7
|
1.0
|
|
CB
|
A:ASP216
|
4.2
|
27.3
|
1.0
|
|
CB
|
A:ASP214
|
4.3
|
31.1
|
1.0
|
|
CB
|
A:ASP222
|
4.3
|
21.3
|
1.0
|
|
CB
|
A:ASP224
|
4.6
|
27.8
|
1.0
|
|
CD1
|
A:ILE218
|
4.7
|
28.6
|
1.0
|
|
OD1
|
A:ASP224
|
4.8
|
40.7
|
1.0
|
|
CB
|
A:ILE218
|
4.9
|
25.4
|
1.0
|
|
O
|
A:ILE218
|
4.9
|
23.9
|
1.0
|
|
OD2
|
A:ASP225
|
4.9
|
23.6
|
1.0
|
|
Calcium binding site 5 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 5 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1005
b:18.6
occ:1.00
|
OE1
|
A:GLN110
|
2.2
|
20.9
|
1.0
|
|
O
|
A:ALA227
|
2.3
|
17.6
|
1.0
|
|
O
|
A:VAL108
|
2.3
|
18.8
|
1.0
|
|
OE2
|
A:GLU229
|
2.3
|
19.0
|
1.0
|
|
OE1
|
A:GLU229
|
2.4
|
20.3
|
1.0
|
|
O
|
A:HOH2008
|
2.6
|
28.2
|
1.0
|
|
CD
|
A:GLU229
|
2.7
|
20.8
|
1.0
|
|
O
|
A:HOH2195
|
3.0
|
23.6
|
1.0
|
|
CD
|
A:GLN110
|
3.3
|
19.5
|
1.0
|
|
C
|
A:ALA227
|
3.5
|
18.2
|
1.0
|
|
C
|
A:VAL108
|
3.5
|
18.9
|
1.0
|
|
NE2
|
A:GLN110
|
3.9
|
21.0
|
1.0
|
|
N
|
A:GLN110
|
4.0
|
16.4
|
1.0
|
|
N
|
A:ALA227
|
4.1
|
18.1
|
1.0
|
|
CG
|
A:GLU229
|
4.2
|
16.4
|
1.0
|
|
CG2
|
A:VAL108
|
4.3
|
20.6
|
1.0
|
|
C
|
A:ASP226
|
4.3
|
19.0
|
1.0
|
|
N
|
A:ILE109
|
4.3
|
18.3
|
1.0
|
|
N
|
A:ALA228
|
4.4
|
18.5
|
1.0
|
|
CA
|
A:ILE109
|
4.4
|
17.5
|
1.0
|
|
CA
|
A:ALA228
|
4.4
|
17.7
|
1.0
|
|
CA
|
A:ALA227
|
4.4
|
17.7
|
1.0
|
|
CA
|
A:VAL108
|
4.4
|
19.8
|
1.0
|
|
C
|
A:ALA228
|
4.5
|
17.8
|
1.0
|
|
C
|
A:ILE109
|
4.5
|
16.9
|
1.0
|
|
CG
|
A:GLN110
|
4.5
|
16.9
|
1.0
|
|
N
|
A:GLU229
|
4.6
|
16.1
|
1.0
|
|
CB
|
A:GLN110
|
4.6
|
16.4
|
1.0
|
|
O
|
A:ASP226
|
4.7
|
18.6
|
1.0
|
|
O
|
A:HOH2204
|
4.7
|
35.5
|
1.0
|
|
CA
|
A:ASP226
|
4.8
|
20.0
|
1.0
|
|
O
|
A:ASP225
|
4.8
|
20.7
|
1.0
|
|
N
|
A:GLY221
|
4.8
|
20.3
|
1.0
|
|
CA
|
A:GLN110
|
4.9
|
15.7
|
1.0
|
|
O
|
A:ALA228
|
4.9
|
18.3
|
1.0
|
|
CB
|
A:VAL108
|
5.0
|
19.6
|
1.0
|
|
Calcium binding site 6 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 6 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1006
b:20.3
occ:1.00
|
O
|
A:LEU373
|
2.3
|
22.5
|
1.0
|
|
OD1
|
A:ASP372
|
2.3
|
25.4
|
1.0
|
|
OD1
|
A:ASP379
|
2.3
|
19.0
|
1.0
|
|
O
|
A:PRO375
|
2.3
|
24.4
|
1.0
|
|
O
|
A:GLY377
|
2.4
|
23.5
|
1.0
|
|
O
|
A:HOH2036
|
2.5
|
20.6
|
1.0
|
|
CG
|
A:ASP379
|
3.5
|
17.8
|
1.0
|
|
CG
|
A:ASP372
|
3.5
|
23.7
|
1.0
|
|
C
|
A:LEU373
|
3.5
|
22.6
|
1.0
|
|
C
|
A:PRO375
|
3.6
|
25.5
|
1.0
|
|
C
|
A:GLY377
|
3.6
|
22.9
|
1.0
|
|
N
|
A:GLY377
|
3.9
|
24.2
|
1.0
|
|
N
|
A:LEU373
|
4.0
|
22.2
|
1.0
|
|
CB
|
A:ASP379
|
4.1
|
18.1
|
1.0
|
|
C
|
A:GLY374
|
4.1
|
24.6
|
1.0
|
|
OD2
|
A:ASP372
|
4.2
|
24.2
|
1.0
|
|
N
|
A:PRO375
|
4.2
|
24.8
|
1.0
|
|
C
|
A:THR376
|
4.2
|
24.9
|
1.0
|
|
O
|
A:GLY383
|
4.2
|
16.1
|
1.0
|
|
N
|
A:ASP379
|
4.2
|
19.4
|
1.0
|
|
C
|
A:ASP372
|
4.2
|
22.3
|
1.0
|
|
CA
|
A:GLY377
|
4.3
|
23.7
|
1.0
|
|
O
|
A:GLY374
|
4.4
|
24.9
|
1.0
|
|
CA
|
A:LEU373
|
4.4
|
22.1
|
1.0
|
|
N
|
A:GLY374
|
4.4
|
23.0
|
1.0
|
|
CA
|
A:GLY374
|
4.4
|
23.7
|
1.0
|
|
N
|
A:THR376
|
4.5
|
24.9
|
1.0
|
|
CA
|
A:PRO375
|
4.5
|
24.9
|
1.0
|
|
C
|
A:TRP378
|
4.5
|
20.8
|
1.0
|
|
CA
|
A:THR376
|
4.5
|
25.3
|
1.0
|
|
OD2
|
A:ASP379
|
4.6
|
16.4
|
1.0
|
|
CA
|
A:ASP372
|
4.6
|
21.6
|
1.0
|
|
N
|
A:TRP378
|
4.6
|
21.8
|
1.0
|
|
CB
|
A:ASP372
|
4.6
|
21.6
|
1.0
|
|
CD
|
A:PRO375
|
4.7
|
24.2
|
1.0
|
|
O
|
A:ASP372
|
4.7
|
22.7
|
1.0
|
|
O
|
A:THR376
|
4.7
|
25.4
|
1.0
|
|
CA
|
A:TRP378
|
4.7
|
20.7
|
1.0
|
|
CA
|
A:ASP379
|
4.8
|
19.1
|
1.0
|
|
O
|
A:TYR384
|
5.0
|
19.5
|
1.0
|
|
Calcium binding site 7 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 7 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca1007
b:19.2
occ:1.00
|
OD1
|
A:ASP121
|
2.2
|
18.2
|
1.0
|
|
OD2
|
A:ASP119
|
2.3
|
16.4
|
1.0
|
|
OD2
|
A:ASP314
|
2.3
|
21.9
|
1.0
|
|
O
|
A:HOH2221
|
2.3
|
22.9
|
1.0
|
|
OD1
|
A:ASP315
|
2.5
|
19.9
|
1.0
|
|
O
|
A:HOH2152
|
2.5
|
28.9
|
1.0
|
|
OD1
|
A:ASP119
|
2.9
|
22.3
|
1.0
|
|
CG
|
A:ASP119
|
2.9
|
19.1
|
1.0
|
|
CG
|
A:ASP315
|
3.3
|
22.7
|
1.0
|
|
CG
|
A:ASP121
|
3.4
|
21.4
|
1.0
|
|
CG
|
A:ASP314
|
3.5
|
21.9
|
1.0
|
|
OD2
|
A:ASP315
|
3.8
|
24.6
|
1.0
|
|
OD2
|
A:ASP121
|
3.9
|
24.1
|
1.0
|
|
N
|
A:ASP315
|
3.9
|
18.4
|
1.0
|
|
OD1
|
A:ASP314
|
4.2
|
23.9
|
1.0
|
|
N
|
A:HIS122
|
4.2
|
18.3
|
1.0
|
|
O
|
A:HOH2243
|
4.2
|
37.1
|
1.0
|
|
O
|
A:HOH2220
|
4.2
|
32.6
|
1.0
|
|
C
|
A:ASP121
|
4.3
|
19.1
|
1.0
|
|
O
|
A:HOH2242
|
4.4
|
52.4
|
1.0
|
|
CB
|
A:ASP119
|
4.4
|
16.6
|
1.0
|
|
C
|
A:ASP314
|
4.4
|
18.8
|
1.0
|
|
CA
|
A:ASP314
|
4.5
|
18.7
|
1.0
|
|
CB
|
A:ASP315
|
4.5
|
20.2
|
1.0
|
|
O
|
A:HOH2115
|
4.5
|
29.3
|
1.0
|
|
CA
|
A:HIS122
|
4.5
|
17.2
|
1.0
|
|
CB
|
A:ASP314
|
4.6
|
19.0
|
1.0
|
|
CA
|
A:ASP315
|
4.6
|
19.7
|
1.0
|
|
CB
|
A:ASP121
|
4.6
|
19.8
|
1.0
|
|
O
|
A:ASP121
|
4.6
|
18.8
|
1.0
|
|
N
|
A:ASP121
|
4.7
|
19.1
|
1.0
|
|
CA
|
A:ASP121
|
4.7
|
19.4
|
1.0
|
|
O
|
A:HOH2251
|
4.8
|
42.1
|
1.0
|
|
Calcium binding site 8 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 8 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca1008
b:19.6
occ:1.00
|
O
|
C:VAL170
|
2.3
|
18.4
|
1.0
|
|
O
|
C:LEU164
|
2.3
|
18.9
|
1.0
|
|
OD1
|
C:ASP124
|
2.3
|
16.9
|
1.0
|
|
OE1
|
C:GLN84
|
2.4
|
16.8
|
1.0
|
|
O
|
C:ILE168
|
2.4
|
20.9
|
1.0
|
|
OD1
|
C:ASN166
|
2.6
|
21.5
|
1.0
|
|
OD2
|
C:ASP124
|
2.6
|
18.4
|
1.0
|
|
CG
|
C:ASP124
|
2.8
|
20.0
|
1.0
|
|
CD
|
C:GLN84
|
3.4
|
17.3
|
1.0
|
|
CG
|
C:ASN166
|
3.5
|
21.8
|
1.0
|
|
C
|
C:VAL170
|
3.5
|
18.2
|
1.0
|
|
C
|
C:LEU164
|
3.5
|
18.0
|
1.0
|
|
C
|
C:ILE168
|
3.6
|
21.6
|
1.0
|
|
N
|
C:VAL170
|
3.7
|
19.2
|
1.0
|
|
CG
|
C:GLN84
|
3.9
|
19.1
|
1.0
|
|
ND2
|
C:ASN166
|
3.9
|
20.3
|
1.0
|
|
N
|
C:ASN166
|
4.0
|
21.8
|
1.0
|
|
CA
|
C:VAL170
|
4.2
|
18.1
|
1.0
|
|
C
|
C:GLY169
|
4.3
|
19.5
|
1.0
|
|
N
|
C:LEU164
|
4.3
|
16.8
|
1.0
|
|
CA
|
C:LEU164
|
4.3
|
17.3
|
1.0
|
|
CB
|
C:ASP124
|
4.4
|
18.9
|
1.0
|
|
N
|
C:ILE168
|
4.4
|
24.0
|
1.0
|
|
N
|
C:ASN165
|
4.4
|
19.5
|
1.0
|
|
N
|
C:GLY169
|
4.5
|
20.7
|
1.0
|
|
CB
|
C:GLN84
|
4.5
|
22.6
|
1.0
|
|
CA
|
C:GLY169
|
4.5
|
19.6
|
1.0
|
|
N
|
C:VAL171
|
4.5
|
17.4
|
1.0
|
|
CA
|
C:ASN165
|
4.5
|
20.8
|
1.0
|
|
CG1
|
C:ILE168
|
4.5
|
22.5
|
1.0
|
|
CA
|
C:ILE168
|
4.5
|
22.1
|
1.0
|
|
NE2
|
C:GLN84
|
4.6
|
18.4
|
1.0
|
|
CA
|
C:VAL171
|
4.6
|
16.6
|
1.0
|
|
CB
|
C:ASN166
|
4.6
|
22.7
|
1.0
|
|
CA
|
C:ASN166
|
4.6
|
23.2
|
1.0
|
|
CB
|
C:LEU164
|
4.7
|
17.8
|
1.0
|
|
C
|
C:ASN165
|
4.7
|
21.4
|
1.0
|
|
C
|
C:ASN166
|
4.7
|
23.7
|
1.0
|
|
C
|
C:ALA163
|
4.8
|
16.4
|
1.0
|
|
O
|
C:ASN166
|
4.9
|
23.4
|
1.0
|
|
CB
|
C:VAL171
|
4.9
|
15.3
|
1.0
|
|
Calcium binding site 9 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 9 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 9 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca1009
b:11.5
occ:1.00
|
O
|
C:HOH2085
|
2.0
|
20.6
|
1.0
|
|
OD2
|
C:ASP226
|
2.0
|
20.1
|
1.0
|
|
O
|
C:VAL210
|
2.1
|
23.4
|
1.0
|
|
O
|
C:LEU205
|
2.1
|
18.4
|
1.0
|
|
OD1
|
C:ASP208
|
2.2
|
23.8
|
1.0
|
|
O
|
C:HOH2063
|
2.2
|
18.9
|
1.0
|
|
CG
|
C:ASP208
|
3.1
|
23.8
|
1.0
|
|
CG
|
C:ASP226
|
3.1
|
19.1
|
1.0
|
|
C
|
C:LEU205
|
3.2
|
17.7
|
1.0
|
|
C
|
C:VAL210
|
3.3
|
22.6
|
1.0
|
|
OD2
|
C:ASP208
|
3.3
|
26.1
|
1.0
|
|
OD1
|
C:ASP226
|
3.6
|
21.4
|
1.0
|
|
N
|
C:VAL210
|
3.8
|
22.6
|
1.0
|
|
N
|
C:ASP226
|
3.9
|
19.1
|
1.0
|
|
CA
|
C:LEU205
|
4.1
|
17.3
|
1.0
|
|
CA
|
C:VAL210
|
4.1
|
22.9
|
1.0
|
|
O
|
C:ALA211
|
4.1
|
21.8
|
1.0
|
|
N
|
C:GLY206
|
4.1
|
17.8
|
1.0
|
|
N
|
C:ALA211
|
4.2
|
22.8
|
1.0
|
|
O
|
C:ILE204
|
4.3
|
16.0
|
1.0
|
|
CA
|
C:GLY206
|
4.3
|
18.1
|
1.0
|
|
CA
|
C:ALA211
|
4.3
|
22.6
|
1.0
|
|
CB
|
C:ASP226
|
4.3
|
19.4
|
1.0
|
|
C
|
C:ALA211
|
4.4
|
22.9
|
1.0
|
|
CA
|
C:ASP226
|
4.4
|
19.5
|
1.0
|
|
CB
|
C:ASP208
|
4.4
|
22.4
|
1.0
|
|
N
|
C:ALA227
|
4.5
|
17.6
|
1.0
|
|
C
|
C:ASP226
|
4.5
|
18.4
|
1.0
|
|
N
|
C:GLY209
|
4.5
|
22.9
|
1.0
|
|
CB
|
C:VAL210
|
4.6
|
23.3
|
1.0
|
|
C
|
C:GLY206
|
4.6
|
19.2
|
1.0
|
|
OD1
|
C:ASP225
|
4.6
|
22.1
|
1.0
|
|
N
|
C:ASP208
|
4.7
|
22.2
|
1.0
|
|
O
|
C:GLY206
|
4.7
|
18.9
|
1.0
|
|
C
|
C:ASP208
|
4.8
|
23.4
|
1.0
|
|
CA
|
C:ASP208
|
4.8
|
23.2
|
1.0
|
|
C
|
C:ASP225
|
4.9
|
20.2
|
1.0
|
|
CB
|
C:ASP225
|
4.9
|
21.2
|
1.0
|
|
C
|
C:GLY209
|
4.9
|
22.1
|
1.0
|
|
CA
|
C:ASP225
|
5.0
|
21.3
|
1.0
|
|
Calcium binding site 10 out
of 14 in 2z2y
Go back to
Calcium Binding Sites List in 2z2y
Calcium binding site 10 out
of 14 in the Crystal Structure of Autoprocessed Form of Tk-Subtilisin
 Mono view
 Stereo pair view
|
|
A full contact list of Calcium with other atoms in the Ca binding
site number 10 of Crystal Structure of Autoprocessed Form of Tk-Subtilisin within 5.0Å range:
|
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
C:Ca1010
b:19.4
occ:1.00
|
OD1
|
C:ASP212
|
2.1
|
16.7
|
1.0
|
|
OD1
|
C:ASP222
|
2.2
|
21.2
|
1.0
|
|
OD2
|
C:ASP225
|
2.3
|
25.4
|
1.0
|
|
O
|
C:ILE218
|
2.3
|
23.5
|
1.0
|
|
OD2
|
C:ASP214
|
2.4
|
26.6
|
1.0
|
|
OD1
|
C:ASP216
|
2.4
|
26.0
|
1.0
|
|
CG
|
C:ASP216
|
3.3
|
26.9
|
1.0
|
|
CG
|
C:ASP222
|
3.3
|
21.0
|
1.0
|
|
CG
|
C:ASP212
|
3.3
|
23.1
|
1.0
|
|
C
|
C:ILE218
|
3.4
|
24.8
|
1.0
|
|
CG
|
C:ASP225
|
3.4
|
22.1
|
1.0
|
|
CG
|
C:ASP214
|
3.4
|
29.1
|
1.0
|
|
OD2
|
C:ASP216
|
3.6
|
25.2
|
1.0
|
|
CA
|
C:CA1011
|
3.7
|
16.5
|
1.0
|
|
OD1
|
C:ASP214
|
3.8
|
28.1
|
1.0
|
|
N
|
C:ILE218
|
3.9
|
26.3
|
1.0
|
|
CB
|
C:ASP222
|
3.9
|
19.5
|
1.0
|
|
OD1
|
C:ASP225
|
4.1
|
22.1
|
1.0
|
|
CA
|
C:ILE218
|
4.1
|
26.0
|
1.0
|
|
OD2
|
C:ASP212
|
4.1
|
24.4
|
1.0
|
|
CA
|
C:ASP212
|
4.2
|
23.5
|
1.0
|
|
N
|
C:ASP216
|
4.3
|
27.9
|
1.0
|
|
CB
|
C:ILE218
|
4.3
|
25.8
|
1.0
|
|
CB
|
C:ASP212
|
4.3
|
22.3
|
1.0
|
|
OD2
|
C:ASP222
|
4.3
|
20.1
|
1.0
|
|
N
|
C:ASP214
|
4.4
|
28.3
|
1.0
|
|
C
|
C:ASP212
|
4.5
|
24.2
|
1.0
|
|
N
|
C:LYS213
|
4.5
|
25.9
|
1.0
|
|
N
|
C:ILE219
|
4.5
|
23.7
|
1.0
|
|
CB
|
C:ASP225
|
4.6
|
21.2
|
1.0
|
|
CB
|
C:ASP216
|
4.6
|
27.4
|
1.0
|
|
N
|
C:GLY215
|
4.7
|
28.8
|
1.0
|
|
N
|
C:GLY217
|
4.7
|
27.7
|
1.0
|
|
CB
|
C:ASP214
|
4.7
|
28.6
|
1.0
|
|
CA
|
C:ASP216
|
4.8
|
27.7
|
1.0
|
|
CA
|
C:ILE219
|
4.8
|
22.6
|
1.0
|
|
C
|
C:ASP214
|
4.9
|
29.0
|
1.0
|
|
CA
|
C:ASP214
|
4.9
|
28.6
|
1.0
|
|
C
|
C:ASP216
|
4.9
|
27.9
|
1.0
|
|
Reference:
S.Tanaka,
H.Matsumura,
Y.Koga,
K.Takano,
S.Kanaya.
Four New Crystal Structures of Tk-Subtilisin in Unautoprocessed, Autoprocessed and Mature Forms: Insight Into Structural Changes During Maturation J.Mol.Biol. V. 372 1055 2007.
ISSN: ISSN 0022-2836
PubMed: 17706669
DOI: 10.1016/J.JMB.2007.07.027
Page generated: Tue Jul 8 09:46:21 2025
|
