Calcium in PDB 4yi8: Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3

The structure of Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3, PDB code: 4yi8 was solved by R.Prem Kumar, M.J.Ranaghan, D.D.Oprian, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.00 / 1.20
Space group	I 4
Cell size a, b, c (Å), α, β, γ (°)	84.160, 84.160, 59.230, 90.00, 90.00, 90.00
R / Rfree (%)	12.1 / 14

The binding sites of Calcium atom in the Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3 (pdb code 4yi8). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3, PDB code: 4yi8:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:12.1 occ:1.00 OD1 A:ASP74 2.3 12.7 1.0 O A:THR80 2.3 11.9 1.0 OD1 A:ASN76 2.4 15.2 1.0 O A:HOH440 2.4 15.3 1.0 OD1 A:ASP78 2.4 13.6 1.0 OE1 A:GLU85 2.4 13.2 1.0 OE2 A:GLU85 2.7 13.4 1.0 CD A:GLU85 2.9 10.9 1.0 CG A:ASP78 3.3 13.6 1.0 H A:ASP78 3.4 18.4 1.0 CG A:ASN76 3.4 15.3 1.0 HA A:ASP74 3.5 14.2 1.0 CG A:ASP74 3.5 13.2 1.0 HD21 A:ASN76 3.5 22.1 1.0 HA A:LEU81 3.5 12.9 1.0 C A:THR80 3.5 10.8 1.0 H A:THR80 3.6 14.7 1.0 H A:ASN76 3.7 19.1 1.0 OD2 A:ASP78 3.7 15.0 1.0 ND2 A:ASN76 3.8 18.4 1.0 H A:ASP82 3.8 12.5 1.0 HG1 A:THR80 4.0 16.8 1.0 HD23 A:LEU81 4.1 16.2 1.0 CA A:ASP74 4.2 11.8 1.0 OG1 A:THR80 4.2 14.0 1.0 N A:ASP78 4.2 15.3 1.0 CA A:LEU81 4.3 10.7 1.0 OD2 A:ASP74 4.3 14.6 1.0 N A:THR80 4.3 12.3 1.0 CB A:ASP74 4.3 13.1 1.0 N A:LEU81 4.3 10.4 1.0 H A:ALA75 4.4 16.2 1.0 CG A:GLU85 4.4 11.7 1.0 CB A:ASP78 4.4 14.5 1.0 C A:ASP74 4.5 12.9 1.0 CA A:THR80 4.5 11.6 1.0 N A:ASN76 4.5 15.9 1.0 N A:ASP82 4.5 10.4 1.0 HB2 A:ASP74 4.5 15.7 1.0 H A:SER77 4.5 19.3 1.0 H A:GLY79 4.6 17.4 1.0 HB3 A:ASP78 4.6 17.4 1.0 N A:ALA75 4.6 13.5 1.0 HD22 A:ASN76 4.7 22.1 1.0 O A:HOH418 4.7 18.6 1.0 CB A:ASN76 4.7 16.7 1.0 N A:SER77 4.7 16.1 1.0 CA A:ASP78 4.7 15.0 1.0 C A:LEU81 4.8 10.4 1.0 HG3 A:GLU85 4.8 14.0 1.0 HB2 A:GLU85 4.9 12.5 1.0 HG2 A:GLU85 4.9 14.0 1.0 C A:ASN76 4.9 17.6 1.0 N A:GLY79 4.9 14.6 1.0 CA A:ASN76 4.9 16.6 1.0 O A:HOH422 5.0 23.4 1.0 CB A:THR80 5.0 13.0 1.0 CD2 A:LEU81 5.0 13.5 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Non-Myristoylated E153A Recoverin at 1.2 A Resolution with Calcium Ions Bound to Ef-Hands 2 and 3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca302 b:11.6 occ:1.00 OD1 A:ASP110 2.3 12.2 1.0 O A:THR116 2.3 13.5 1.0 O A:HOH515 2.4 15.1 0.8 OD1 A:ASN114 2.4 14.1 1.0 OD1 A:ASP112 2.4 14.8 1.0 O A:HOH515 2.4 13.8 0.2 OE1 A:GLU121 2.5 11.3 1.0 OE2 A:GLU121 2.5 11.9 1.0 CD A:GLU121 2.9 10.8 1.0 CG A:ASN114 3.3 15.6 1.0 H A:ASN114 3.3 18.4 1.0 CG A:ASP112 3.4 15.5 1.0 CG A:ASP110 3.5 12.1 1.0 H A:ASP112 3.5 15.5 1.0 C A:THR116 3.5 10.7 1.0 HA A:ASP110 3.6 12.6 1.0 HA A:ILE117 3.6 11.5 1.0 H A:THR116 3.6 14.3 1.0 HD21 A:ASN114 3.7 23.9 1.0 ND2 A:ASN114 3.9 19.9 1.0 OD2 A:ASP112 4.0 19.3 1.0 H A:SER118 4.0 12.0 1.0 H A:GLY113 4.1 17.3 1.0 N A:ASN114 4.1 15.3 1.0 CA A:ASP110 4.2 10.5 1.0 N A:ASP112 4.2 12.9 1.0 O A:HOH548 4.2 25.8 1.0 OD2 A:ASP110 4.2 12.7 1.0 N A:THR116 4.3 11.9 1.0 C A:ASP110 4.3 10.7 1.0 CA A:ILE117 4.4 9.6 1.0 CB A:ASP110 4.4 11.2 1.0 CG A:GLU121 4.4 11.4 1.0 O A:HOH551 4.4 30.9 1.0 CB A:ASN114 4.4 16.7 1.0 N A:ILE117 4.4 9.8 1.0 H A:VAL111 4.4 13.5 1.0 H A:GLY115 4.4 17.1 1.0 HB3 A:ASN114 4.5 20.0 1.0 N A:GLY113 4.5 14.4 1.0 N A:VAL111 4.5 11.2 1.0 CA A:THR116 4.5 11.8 1.0 CB A:ASP112 4.5 16.0 1.0 N A:SER118 4.6 10.0 1.0 HG1 A:THR116 4.6 17.9 1.0 HB2 A:ASP110 4.6 13.4 1.0 HG3 A:GLU121 4.6 13.7 1.0 CA A:ASN114 4.7 16.0 1.0 HD22 A:ASN114 4.7 23.9 1.0 HB3 A:SER118 4.7 15.8 1.0 CA A:ASP112 4.7 14.4 1.0 OG1 A:THR116 4.7 14.9 1.0 N A:GLY115 4.8 14.2 1.0 HG2 A:GLU121 4.8 13.7 1.0 C A:ASP112 4.8 15.0 1.0 HB3 A:ASP112 4.8 19.2 1.0 O A:ASP110 4.8 12.4 1.0 C A:ILE117 4.9 9.8 1.0 O A:HOH532 4.9 21.2 1.0 C A:ASN114 4.9 15.2 1.0

R.P.Kumar, M.J.Ranaghan, A.Y.Ganjei, D.D.Oprian. Crystal Structure of Recoverin with Calcium Ions Bound to Both Functional Ef Hands. Biochemistry V. 54 7222 2015.
ISSN: ISSN 0006-2960
PubMed: 26584024
DOI: 10.1021/ACS.BIOCHEM.5B01160