Calcium in PDB 5k7s: Microed Structure of Proteinase K at 1.6 A Resolution

All present enzymatic activity of Microed Structure of Proteinase K at 1.6 A Resolution:
3.4.21.64;

The binding sites of Calcium atom in the Microed Structure of Proteinase K at 1.6 A Resolution (pdb code 5k7s). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Microed Structure of Proteinase K at 1.6 A Resolution, PDB code: 5k7s:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Microed Structure of Proteinase K at 1.6 A Resolution


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Microed Structure of Proteinase K at 1.6 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca401 b:15.0 occ:1.00 ND1 A:HIS177 2.3 6.7 1.0 SG A:CYS178 2.6 13.5 1.0 O A:HOH501 2.8 6.3 1.0 SD A:MET330 2.8 16.6 1.0 N A:CYS178 2.9 3.1 1.0 O A:HOH550 3.0 8.9 1.0 CG A:HIS177 3.2 7.2 1.0 CA A:CYS178 3.2 3.8 1.0 C A:HIS177 3.2 3.1 1.0 CE1 A:HIS177 3.3 4.5 1.0 CB A:HIS177 3.3 3.6 1.0 CB A:CYS178 3.5 7.6 1.0 CG A:MET330 3.5 13.0 1.0 O A:HIS177 3.7 4.1 1.0 O A:HIS174 3.9 3.0 1.0 CA A:HIS177 3.9 3.2 1.0 CD2 A:HIS177 4.3 4.7 1.0 CA A:MET330 4.4 8.3 1.0 NE2 A:HIS177 4.4 4.2 1.0 O A:SER329 4.4 10.9 1.0 CE A:MET330 4.5 17.9 1.0 CB A:MET330 4.5 6.5 1.0 CD2 A:HIS174 4.6 25.5 1.0 CD1 A:ILE325 4.6 11.7 1.0 OG1 A:THR181 4.6 3.8 1.0 C A:CYS178 4.6 2.9 1.0 CG A:PRO333 4.7 4.5 1.0 N A:HIS177 4.8 2.7 1.0 N A:MET330 4.9 9.3 1.0 C A:SER329 4.9 10.4 1.0 CG1 A:ILE325 4.9 10.5 1.0 C A:HIS174 5.0 4.6 1.0

Calcium binding site 2 out of 2 in the Microed Structure of Proteinase K at 1.6 A Resolution


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Microed Structure of Proteinase K at 1.6 A Resolution within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:22.9 occ:1.00 O A:PRO280 2.4 10.1 1.0 O A:VAL282 2.4 7.2 1.0 OD2 A:ASP305 2.5 11.8 1.0 O A:HOH551 2.5 11.0 1.0 O A:HOH527 2.6 14.1 1.0 OD1 A:ASP305 2.6 10.5 1.0 O A:HOH656 2.6 11.0 1.0 CG A:ASP305 2.8 11.3 1.0 C A:PRO280 3.4 9.9 1.0 C A:VAL282 3.6 10.1 1.0 CA A:PRO280 4.0 11.9 1.0 O A:VAL303 4.1 9.3 1.0 N A:VAL282 4.2 7.8 1.0 O A:GLU279 4.2 8.1 1.0 O A:HOH701 4.3 15.8 1.0 CB A:ASP305 4.3 9.8 1.0 C A:SER281 4.4 7.5 1.0 N A:SER281 4.4 8.2 1.0 O A:HOH684 4.5 16.9 1.0 CA A:CYS283 4.5 8.6 1.0 N A:CYS283 4.5 8.8 1.0 N A:THR284 4.5 6.7 1.0 CA A:VAL282 4.6 5.1 1.0 OG1 A:THR284 4.6 5.5 1.0 CA A:SER281 4.7 11.8 1.0 SG A:CYS354 4.9 16.4 1.0 O A:HOH583 4.9 12.1 1.0 O A:SER281 4.9 9.0 1.0

M.J.De La Cruz, J.Hattne, D.Shi, P.Seidler, J.Rodriguez, F.E.Reyes, M.R.Sawaya, D.Cascio, S.C.Weiss, S.K.Kim, C.S.Hinck, A.P.Hinck, G.Calero, D.Eisenberg, T.Gonen. Atomic-Resolution Structures From Fragmented Protein Crystals with the Cryoem Method Microed. Nat. Methods V. 14 399 2017.
ISSN: ESSN 1548-7105
PubMed: 28192420
DOI: 10.1038/NMETH.4178