Calcium in PDB 5k8q: Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide.
Protein crystallography data
The structure of Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide., PDB code: 5k8q
was solved by
S.D.Stowe,
O.B.Clarke,
M.C.Cavalier,
R.Godoy-Ruiz,
F.Mancia,
D.J.Weber,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
25.37 /
1.74
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
156.510,
37.230,
35.730,
90.00,
94.35,
90.00
|
R / Rfree (%)
|
22.7 /
26.7
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide.
(pdb code 5k8q). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 4 binding sites of Calcium where determined in the
Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide., PDB code: 5k8q:
Jump to Calcium binding site number:
1;
2;
3;
4;
Calcium binding site 1 out
of 4 in 5k8q
Go back to
Calcium Binding Sites List in 5k8q
Calcium binding site 1 out
of 4 in the Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide.
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca201
b:20.2
occ:1.00
|
OD1
|
A:ASP129
|
2.2
|
27.9
|
1.0
|
O
|
A:GLN135
|
2.3
|
19.4
|
1.0
|
OD2
|
A:ASP133
|
2.3
|
20.2
|
1.0
|
O
|
A:HOH307
|
2.4
|
22.2
|
1.0
|
OD2
|
A:ASP131
|
2.4
|
27.4
|
1.0
|
OE2
|
A:GLU140
|
2.4
|
15.1
|
1.0
|
OE1
|
A:GLU140
|
2.5
|
23.1
|
1.0
|
CD
|
A:GLU140
|
2.8
|
16.3
|
1.0
|
CG
|
A:ASP131
|
3.1
|
23.5
|
1.0
|
CG
|
A:ASP133
|
3.2
|
30.7
|
1.0
|
OD1
|
A:ASP131
|
3.4
|
28.0
|
1.0
|
CG
|
A:ASP129
|
3.4
|
25.9
|
1.0
|
C
|
A:GLN135
|
3.4
|
12.8
|
1.0
|
OD1
|
A:ASP133
|
3.6
|
21.6
|
1.0
|
CA
|
A:ASP129
|
4.1
|
19.8
|
1.0
|
N
|
A:ASP133
|
4.1
|
26.5
|
1.0
|
CB
|
A:ASP129
|
4.2
|
23.9
|
1.0
|
N
|
A:GLN135
|
4.2
|
20.9
|
1.0
|
N
|
A:ASP131
|
4.2
|
30.5
|
1.0
|
N
|
A:VAL136
|
4.2
|
12.5
|
1.0
|
OD2
|
A:ASP129
|
4.3
|
24.9
|
1.0
|
CA
|
A:VAL136
|
4.3
|
15.3
|
1.0
|
CB
|
A:ASP133
|
4.3
|
24.2
|
1.0
|
CG
|
A:GLU140
|
4.3
|
23.8
|
1.0
|
N
|
A:ASN137
|
4.3
|
13.5
|
1.0
|
CB
|
A:ASP131
|
4.4
|
24.4
|
1.0
|
CA
|
A:GLN135
|
4.4
|
20.2
|
1.0
|
C
|
A:ASP129
|
4.4
|
23.3
|
1.0
|
N
|
A:ILE130
|
4.5
|
28.0
|
1.0
|
N
|
A:GLY132
|
4.5
|
30.6
|
1.0
|
CA
|
A:ASP133
|
4.7
|
27.8
|
1.0
|
CA
|
A:ASP131
|
4.7
|
27.0
|
1.0
|
C
|
A:VAL136
|
4.8
|
16.2
|
1.0
|
C
|
A:ASP131
|
4.8
|
30.9
|
1.0
|
N
|
A:GLY134
|
4.8
|
21.6
|
1.0
|
CG
|
A:ASN137
|
4.9
|
25.9
|
1.0
|
ND2
|
A:ASN137
|
4.9
|
20.2
|
1.0
|
C
|
A:ASP133
|
4.9
|
20.5
|
1.0
|
|
Calcium binding site 2 out
of 4 in 5k8q
Go back to
Calcium Binding Sites List in 5k8q
Calcium binding site 2 out
of 4 in the Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide.
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca202
b:18.1
occ:1.00
|
OD2
|
A:ASP93
|
2.2
|
26.1
|
1.0
|
O
|
A:TYR99
|
2.2
|
19.9
|
1.0
|
OD1
|
A:ASP95
|
2.4
|
31.0
|
1.0
|
OD1
|
A:ASN97
|
2.4
|
22.2
|
1.0
|
O
|
A:HOH368
|
2.4
|
24.3
|
1.0
|
OE2
|
A:GLU104
|
2.5
|
24.7
|
1.0
|
OE1
|
A:GLU104
|
2.5
|
29.6
|
1.0
|
CD
|
A:GLU104
|
2.9
|
22.4
|
1.0
|
CG
|
A:ASN97
|
3.3
|
18.8
|
1.0
|
CG
|
A:ASP93
|
3.4
|
22.4
|
1.0
|
C
|
A:TYR99
|
3.4
|
26.9
|
1.0
|
CG
|
A:ASP95
|
3.5
|
36.5
|
1.0
|
ND2
|
A:ASN97
|
3.9
|
19.7
|
1.0
|
OD2
|
A:ASP95
|
4.0
|
33.5
|
1.0
|
N
|
A:TYR99
|
4.1
|
22.2
|
1.0
|
OD1
|
A:ASP93
|
4.2
|
25.6
|
1.0
|
CA
|
A:ASP93
|
4.2
|
23.1
|
1.0
|
CB
|
A:ASP93
|
4.2
|
15.5
|
1.0
|
CA
|
A:TYR99
|
4.3
|
26.6
|
1.0
|
N
|
A:ASN97
|
4.3
|
20.8
|
1.0
|
CG
|
A:GLU104
|
4.4
|
18.4
|
1.0
|
N
|
A:ASP95
|
4.4
|
28.9
|
1.0
|
N
|
A:ILE100
|
4.4
|
20.2
|
1.0
|
O
|
A:HOH381
|
4.4
|
40.7
|
1.0
|
CB
|
A:ASN97
|
4.5
|
19.1
|
1.0
|
CA
|
A:ILE100
|
4.5
|
18.3
|
1.0
|
C
|
A:ASP93
|
4.5
|
25.8
|
1.0
|
N
|
A:LYS94
|
4.6
|
26.0
|
0.6
|
N
|
A:GLY96
|
4.6
|
25.9
|
1.0
|
N
|
A:LYS94
|
4.6
|
26.4
|
0.4
|
N
|
A:SER101
|
4.6
|
14.9
|
1.0
|
CB
|
A:TYR99
|
4.6
|
19.2
|
1.0
|
CB
|
A:ASP95
|
4.7
|
27.4
|
1.0
|
OE1
|
A:GLN135
|
4.7
|
25.7
|
1.0
|
CA
|
A:ASN97
|
4.8
|
23.8
|
1.0
|
CA
|
A:ASP95
|
4.8
|
23.6
|
1.0
|
C
|
A:ASP95
|
4.9
|
25.4
|
1.0
|
N
|
A:GLY98
|
4.9
|
21.8
|
1.0
|
OG
|
A:SER101
|
5.0
|
14.8
|
1.0
|
C
|
A:ILE100
|
5.0
|
12.6
|
1.0
|
|
Calcium binding site 3 out
of 4 in 5k8q
Go back to
Calcium Binding Sites List in 5k8q
Calcium binding site 3 out
of 4 in the Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide.
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca203
b:20.6
occ:1.00
|
O
|
A:THR62
|
2.3
|
19.6
|
1.0
|
OD2
|
A:ASP56
|
2.3
|
25.8
|
1.0
|
OE2
|
A:GLU67
|
2.3
|
25.5
|
1.0
|
OD1
|
A:ASP58
|
2.4
|
32.6
|
1.0
|
OD1
|
A:ASN60
|
2.4
|
27.5
|
1.0
|
O
|
A:HOH315
|
2.4
|
39.5
|
1.0
|
OE1
|
A:GLU67
|
2.6
|
21.6
|
1.0
|
O
|
A:HOH346
|
2.6
|
36.3
|
0.7
|
CD
|
A:GLU67
|
2.8
|
21.7
|
1.0
|
CG
|
A:ASP58
|
3.4
|
32.4
|
1.0
|
CG
|
A:ASN60
|
3.4
|
27.1
|
1.0
|
C
|
A:THR62
|
3.5
|
23.3
|
1.0
|
CG
|
A:ASP56
|
3.5
|
25.7
|
1.0
|
OD2
|
A:ASP58
|
3.7
|
28.4
|
1.0
|
ND2
|
A:ASN60
|
3.8
|
19.1
|
1.0
|
OD1
|
A:ASP56
|
4.3
|
33.5
|
1.0
|
N
|
A:THR62
|
4.3
|
29.7
|
1.0
|
CG
|
A:GLU67
|
4.3
|
15.7
|
1.0
|
N
|
A:ILE63
|
4.3
|
20.5
|
1.0
|
N
|
A:ASP58
|
4.4
|
24.4
|
1.0
|
CA
|
A:ILE63
|
4.4
|
23.0
|
1.0
|
OG1
|
A:THR62
|
4.4
|
22.5
|
1.0
|
CA
|
A:ASP56
|
4.4
|
23.7
|
1.0
|
N
|
A:ASN60
|
4.4
|
26.7
|
1.0
|
N
|
A:ASP64
|
4.5
|
15.8
|
1.0
|
CA
|
A:THR62
|
4.5
|
25.8
|
1.0
|
N
|
A:ALA57
|
4.5
|
25.1
|
1.0
|
CB
|
A:ASP56
|
4.5
|
25.9
|
1.0
|
CB
|
A:ASP58
|
4.7
|
27.4
|
1.0
|
CB
|
A:ASN60
|
4.7
|
32.8
|
1.0
|
N
|
A:GLY59
|
4.8
|
40.7
|
1.0
|
OD1
|
A:ASP64
|
4.8
|
36.4
|
1.0
|
C
|
A:ASP56
|
4.8
|
19.0
|
1.0
|
C5
|
A:IMD205
|
4.8
|
35.7
|
1.0
|
C
|
A:ILE63
|
4.9
|
16.9
|
1.0
|
CG
|
A:ASP64
|
4.9
|
28.7
|
1.0
|
CA
|
A:ASP58
|
4.9
|
31.0
|
1.0
|
N
|
A:GLY61
|
4.9
|
26.9
|
1.0
|
CA
|
A:ASN60
|
5.0
|
28.3
|
1.0
|
|
Calcium binding site 4 out
of 4 in 5k8q
Go back to
Calcium Binding Sites List in 5k8q
Calcium binding site 4 out
of 4 in the Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide.
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Crystal Structure of Calcium-Loaded Calmodulin in Complex with STRA6 CAMBP2-Site Peptide. within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca204
b:20.9
occ:1.00
|
OD2
|
A:ASP20
|
2.3
|
22.7
|
1.0
|
O
|
A:THR26
|
2.3
|
30.9
|
1.0
|
OD1
|
A:ASP22
|
2.3
|
24.4
|
1.0
|
OE1
|
A:GLU31
|
2.3
|
30.1
|
1.0
|
OD1
|
A:ASP24
|
2.4
|
31.7
|
1.0
|
O
|
A:HOH350
|
2.4
|
18.8
|
1.0
|
OE2
|
A:GLU31
|
2.5
|
23.6
|
1.0
|
CD
|
A:GLU31
|
2.7
|
26.4
|
1.0
|
CG
|
A:ASP22
|
3.3
|
28.7
|
1.0
|
CG
|
A:ASP24
|
3.3
|
28.7
|
1.0
|
CG
|
A:ASP20
|
3.4
|
24.8
|
1.0
|
C
|
A:THR26
|
3.5
|
26.3
|
1.0
|
OD2
|
A:ASP22
|
3.6
|
37.2
|
1.0
|
OD2
|
A:ASP24
|
3.8
|
34.4
|
1.0
|
OG1
|
A:THR26
|
4.0
|
40.8
|
1.0
|
CA
|
A:ASP20
|
4.1
|
23.5
|
1.0
|
N
|
A:THR26
|
4.2
|
23.4
|
1.0
|
CB
|
A:ASP20
|
4.2
|
17.9
|
1.0
|
CG
|
A:GLU31
|
4.3
|
19.9
|
1.0
|
OD1
|
A:ASP20
|
4.3
|
24.2
|
1.0
|
CA
|
A:THR26
|
4.4
|
21.5
|
1.0
|
N
|
A:ASP24
|
4.4
|
32.3
|
1.0
|
N
|
A:ILE27
|
4.4
|
17.7
|
1.0
|
CB
|
A:ASP24
|
4.5
|
24.7
|
1.0
|
CA
|
A:ILE27
|
4.5
|
18.5
|
1.0
|
C
|
A:ASP20
|
4.6
|
31.0
|
1.0
|
N
|
A:ASP22
|
4.6
|
35.1
|
1.0
|
CB
|
A:ASP22
|
4.7
|
28.8
|
1.0
|
CG2
|
A:THR28
|
4.7
|
26.9
|
1.0
|
O
|
A:HOH318
|
4.8
|
25.4
|
1.0
|
CB
|
A:THR26
|
4.8
|
27.0
|
1.0
|
N
|
A:GLY23
|
4.8
|
39.6
|
1.0
|
N
|
A:THR28
|
4.9
|
17.4
|
1.0
|
CA
|
A:ASP24
|
4.9
|
27.8
|
1.0
|
N
|
A:LYS21
|
4.9
|
33.7
|
1.0
|
N
|
A:GLY25
|
4.9
|
30.7
|
1.0
|
CA
|
A:ASP22
|
5.0
|
34.1
|
1.0
|
|
Reference:
Y.Chen,
O.B.Clarke,
J.Kim,
S.Stowe,
Y.K.Kim,
Z.Assur,
M.Cavalier,
R.Godoy-Ruiz,
D.C.Von Alpen,
C.Manzini,
W.S.Blaner,
J.Frank,
L.Quadro,
D.J.Weber,
L.Shapiro,
W.A.Hendrickson,
F.Mancia.
Structure of the STRA6 Receptor For Retinol Uptake. Science V. 353 2016.
ISSN: ESSN 1095-9203
PubMed: 27563101
DOI: 10.1126/SCIENCE.AAD8266
Page generated: Mon Jul 15 06:43:42 2024
|