Calcium in PDB 5n7f: Magi-1 Complexed with A PRSK1 Peptide
Enzymatic activity of Magi-1 Complexed with A PRSK1 Peptide
All present enzymatic activity of Magi-1 Complexed with A PRSK1 Peptide:
2.7.11.1;
Protein crystallography data
The structure of Magi-1 Complexed with A PRSK1 Peptide, PDB code: 5n7f
was solved by
G.Gogl,
L.Nyitray,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
49.73 /
2.30
|
Space group
|
P 2 21 21
|
Cell size a, b, c (Å), α, β, γ (°)
|
60.150,
98.560,
200.310,
90.00,
90.00,
90.00
|
R / Rfree (%)
|
18.6 /
24
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Magi-1 Complexed with A PRSK1 Peptide
(pdb code 5n7f). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 9 binding sites of Calcium where determined in the
Magi-1 Complexed with A PRSK1 Peptide, PDB code: 5n7f:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
Calcium binding site 1 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 1 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca904
b:42.8
occ:1.00
|
O
|
A:GLY589
|
2.4
|
67.2
|
1.0
|
O
|
A:VAL590
|
2.5
|
24.5
|
1.0
|
O
|
A:HOH1104
|
2.5
|
52.8
|
1.0
|
OE1
|
A:GLU592
|
2.5
|
30.9
|
1.0
|
OE2
|
A:GLU592
|
2.8
|
29.8
|
1.0
|
CD
|
A:GLU592
|
3.0
|
25.7
|
1.0
|
O
|
A:HOH1332
|
3.0
|
31.1
|
1.0
|
C
|
A:GLY589
|
3.3
|
45.3
|
1.0
|
C
|
A:VAL590
|
3.6
|
25.7
|
1.0
|
N
|
A:VAL590
|
4.0
|
39.2
|
1.0
|
CA
|
A:VAL590
|
4.1
|
30.1
|
1.0
|
O
|
A:HOH1300
|
4.1
|
44.5
|
1.0
|
CA
|
A:GLY589
|
4.3
|
37.5
|
1.0
|
O
|
A:HOH1220
|
4.4
|
43.9
|
1.0
|
CB
|
A:VAL590
|
4.4
|
27.3
|
1.0
|
O
|
A:HOH1071
|
4.4
|
36.6
|
1.0
|
CG
|
A:GLU592
|
4.5
|
23.4
|
1.0
|
N
|
A:ASP591
|
4.7
|
25.9
|
1.0
|
N
|
A:GLU592
|
4.8
|
23.3
|
1.0
|
O
|
A:HOH1033
|
4.9
|
22.7
|
1.0
|
|
Calcium binding site 2 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 2 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca905
b:72.0
occ:1.00
|
O
|
A:HOH1085
|
2.5
|
46.4
|
1.0
|
O
|
A:ARG744
|
2.5
|
84.4
|
1.0
|
OE1
|
A:GLU786
|
2.5
|
72.4
|
1.0
|
O
|
A:GLY746
|
2.5
|
63.6
|
1.0
|
O
|
A:GLY741
|
2.6
|
79.2
|
1.0
|
OE2
|
A:GLU786
|
3.0
|
71.9
|
1.0
|
CD
|
A:GLU786
|
3.1
|
69.6
|
1.0
|
O
|
A:VAL742
|
3.6
|
82.7
|
1.0
|
C
|
A:GLY746
|
3.6
|
73.5
|
1.0
|
C
|
A:GLY741
|
3.7
|
74.2
|
1.0
|
C
|
A:ARG744
|
3.7
|
88.6
|
1.0
|
N
|
A:GLY746
|
4.0
|
73.7
|
1.0
|
C
|
A:VAL742
|
4.1
|
81.2
|
1.0
|
CA
|
A:VAL742
|
4.1
|
80.8
|
1.0
|
CA
|
A:GLY746
|
4.2
|
69.5
|
1.0
|
N
|
A:VAL742
|
4.3
|
77.6
|
1.0
|
C
|
A:LYS745
|
4.3
|
84.8
|
1.0
|
N
|
A:ARG744
|
4.3
|
79.6
|
1.0
|
O
|
A:HOH1184
|
4.5
|
53.5
|
1.0
|
CA
|
A:ARG744
|
4.6
|
83.2
|
1.0
|
CG
|
A:GLU786
|
4.6
|
58.4
|
1.0
|
N
|
A:LYS745
|
4.7
|
93.9
|
1.0
|
CA
|
A:GLY741
|
4.7
|
61.7
|
1.0
|
CA
|
A:LYS745
|
4.7
|
91.0
|
1.0
|
N
|
A:THR747
|
4.8
|
75.3
|
1.0
|
O
|
A:LYS745
|
4.9
|
86.4
|
1.0
|
|
Calcium binding site 3 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 3 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca906
b:32.8
occ:1.00
|
OG
|
A:SER773
|
2.5
|
42.3
|
1.0
|
O
|
A:HOH1178
|
2.6
|
35.8
|
1.0
|
CB
|
A:SER773
|
3.5
|
41.1
|
1.0
|
CA
|
A:SER773
|
3.9
|
41.2
|
1.0
|
O
|
A:SER773
|
4.5
|
45.2
|
1.0
|
NH1
|
A:ARG770
|
4.6
|
41.4
|
1.0
|
O
|
A:HOH1131
|
4.7
|
37.8
|
1.0
|
C
|
A:SER773
|
4.7
|
46.9
|
1.0
|
|
Calcium binding site 4 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 4 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca907
b:66.2
occ:1.00
|
O
|
A:HOH1194
|
2.6
|
42.5
|
1.0
|
O
|
A:ASP815
|
2.9
|
24.2
|
1.0
|
N
|
A:GLY819
|
3.1
|
23.9
|
1.0
|
O
|
A:HOH1289
|
3.2
|
53.3
|
1.0
|
O
|
A:SER816
|
3.5
|
27.6
|
1.0
|
N
|
A:LYS818
|
3.5
|
21.0
|
1.0
|
C
|
A:SER816
|
3.6
|
19.2
|
1.0
|
C
|
A:MET817
|
3.6
|
20.4
|
1.0
|
CA
|
A:GLY819
|
3.7
|
25.6
|
1.0
|
N
|
A:MET817
|
3.8
|
21.2
|
1.0
|
C
|
A:ASP815
|
4.0
|
20.6
|
1.0
|
CA
|
A:MET817
|
4.0
|
23.4
|
1.0
|
O
|
A:MET817
|
4.1
|
20.9
|
1.0
|
C
|
A:GLY819
|
4.1
|
25.5
|
1.0
|
C
|
A:LYS818
|
4.1
|
23.6
|
1.0
|
CG
|
A:LYS818
|
4.1
|
23.2
|
1.0
|
O
|
A:GLY819
|
4.3
|
26.0
|
1.0
|
CA
|
A:LYS818
|
4.3
|
22.1
|
1.0
|
CA
|
A:SER816
|
4.3
|
22.4
|
1.0
|
O
|
A:GLY821
|
4.4
|
21.9
|
1.0
|
O
|
A:HOH1290
|
4.4
|
57.0
|
1.0
|
O
|
A:LYS820
|
4.4
|
28.7
|
1.0
|
N
|
A:SER816
|
4.6
|
21.3
|
1.0
|
N
|
A:LYS820
|
4.8
|
28.6
|
1.0
|
CB
|
A:LYS818
|
4.8
|
22.7
|
1.0
|
|
Calcium binding site 5 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 5 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca908
b:97.0
occ:1.00
|
OD1
|
A:ASP524
|
3.2
|
39.0
|
1.0
|
OG
|
A:SER521
|
3.5
|
36.3
|
1.0
|
O
|
A:HOH1305
|
3.5
|
43.2
|
1.0
|
CA
|
A:ASP524
|
3.8
|
37.5
|
1.0
|
OE2
|
A:GLU551
|
3.8
|
35.8
|
1.0
|
C
|
A:ASP524
|
4.1
|
36.2
|
1.0
|
CB
|
A:SER521
|
4.1
|
33.4
|
1.0
|
CG
|
A:ASP524
|
4.2
|
35.3
|
1.0
|
O
|
A:ASP524
|
4.2
|
36.5
|
1.0
|
O
|
A:HOH1120
|
4.4
|
21.2
|
1.0
|
CB
|
A:ASP524
|
4.6
|
34.2
|
1.0
|
N
|
A:ASP524
|
4.7
|
30.1
|
1.0
|
N
|
A:THR525
|
4.8
|
31.1
|
1.0
|
CD
|
A:GLU551
|
4.9
|
41.7
|
1.0
|
|
Calcium binding site 6 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 6 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca904
b:0.2
occ:1.00
|
O
|
B:VAL590
|
2.6
|
43.8
|
1.0
|
CG
|
B:GLU592
|
3.3
|
51.8
|
1.0
|
O
|
B:HOH1029
|
3.5
|
34.3
|
1.0
|
CD
|
B:GLU592
|
3.5
|
69.9
|
1.0
|
OE2
|
B:GLU592
|
3.5
|
78.3
|
1.0
|
O
|
B:GLY589
|
3.6
|
81.9
|
1.0
|
C
|
B:VAL590
|
3.8
|
41.5
|
1.0
|
N
|
B:GLU592
|
4.0
|
38.6
|
1.0
|
CB
|
B:GLU592
|
4.1
|
41.7
|
1.0
|
OE1
|
B:GLU592
|
4.1
|
74.5
|
1.0
|
C
|
B:GLY589
|
4.4
|
72.7
|
1.0
|
O
|
B:HOH1004
|
4.6
|
53.9
|
1.0
|
CA
|
B:VAL590
|
4.6
|
47.1
|
1.0
|
N
|
B:ASP591
|
4.7
|
35.2
|
1.0
|
N
|
B:VAL590
|
4.8
|
58.7
|
1.0
|
CA
|
B:GLU592
|
4.8
|
36.8
|
1.0
|
CB
|
B:VAL590
|
4.8
|
36.6
|
1.0
|
CA
|
B:ASP591
|
4.8
|
34.1
|
1.0
|
C
|
B:ASP591
|
4.9
|
40.1
|
1.0
|
|
Calcium binding site 7 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 7 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca905
b:37.9
occ:1.00
|
O
|
B:HOH1188
|
2.3
|
39.4
|
1.0
|
O
|
B:ARG744
|
2.3
|
39.8
|
1.0
|
O
|
B:GLY741
|
2.3
|
28.7
|
1.0
|
O
|
B:GLY746
|
2.4
|
40.0
|
1.0
|
OE1
|
B:GLU786
|
2.5
|
28.2
|
1.0
|
OE2
|
B:GLU786
|
2.6
|
35.1
|
1.0
|
O
|
B:HOH1200
|
2.7
|
45.2
|
1.0
|
CD
|
B:GLU786
|
2.9
|
35.6
|
1.0
|
C
|
B:GLY741
|
3.4
|
31.1
|
1.0
|
C
|
B:ARG744
|
3.5
|
39.0
|
1.0
|
C
|
B:GLY746
|
3.6
|
40.8
|
1.0
|
N
|
B:GLY746
|
3.8
|
43.2
|
1.0
|
C
|
B:LYS745
|
4.0
|
42.6
|
1.0
|
O
|
B:HOH1110
|
4.1
|
46.7
|
1.0
|
CA
|
B:VAL742
|
4.2
|
35.9
|
1.0
|
N
|
B:VAL742
|
4.2
|
29.1
|
1.0
|
CA
|
B:GLY746
|
4.2
|
43.7
|
1.0
|
CA
|
B:GLY741
|
4.2
|
26.6
|
1.0
|
CA
|
B:LYS745
|
4.3
|
43.3
|
1.0
|
O
|
B:HOH1185
|
4.3
|
41.7
|
1.0
|
O
|
B:VAL742
|
4.4
|
41.5
|
1.0
|
N
|
B:LYS745
|
4.4
|
39.3
|
1.0
|
OG1
|
B:THR747
|
4.4
|
30.6
|
1.0
|
CG
|
B:GLU786
|
4.4
|
35.0
|
1.0
|
N
|
B:ARG744
|
4.4
|
33.5
|
1.0
|
C
|
B:VAL742
|
4.4
|
37.4
|
1.0
|
CA
|
B:ARG744
|
4.6
|
35.8
|
1.0
|
CG
|
B:ARG744
|
4.6
|
41.0
|
1.0
|
O
|
B:LYS745
|
4.6
|
46.9
|
1.0
|
O
|
B:HOH1148
|
4.6
|
46.0
|
1.0
|
N
|
B:THR747
|
4.7
|
38.1
|
1.0
|
CA
|
B:THR747
|
4.9
|
35.5
|
1.0
|
O
|
B:HOH1228
|
5.0
|
41.2
|
1.0
|
|
Calcium binding site 8 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 8 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca906
b:29.9
occ:1.00
|
OG
|
B:SER773
|
2.3
|
27.1
|
1.0
|
O
|
B:HOH1128
|
2.5
|
21.9
|
1.0
|
CB
|
B:SER773
|
3.4
|
26.3
|
1.0
|
CA
|
B:SER773
|
3.9
|
25.3
|
1.0
|
NH1
|
B:ARG770
|
4.3
|
29.1
|
1.0
|
O
|
B:SER773
|
4.4
|
29.5
|
1.0
|
O
|
B:HOH1040
|
4.5
|
18.4
|
1.0
|
C
|
B:SER773
|
4.6
|
31.1
|
1.0
|
|
Calcium binding site 9 out
of 9 in 5n7f
Go back to
Calcium Binding Sites List in 5n7f
Calcium binding site 9 out
of 9 in the Magi-1 Complexed with A PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 9 of Magi-1 Complexed with A PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca907
b:81.3
occ:1.00
|
O
|
B:HOH1107
|
2.3
|
31.7
|
1.0
|
O
|
B:LYS820
|
3.6
|
32.9
|
1.0
|
O
|
B:SER816
|
4.1
|
36.6
|
1.0
|
N
|
B:ARG823
|
4.1
|
29.6
|
1.0
|
O
|
B:ASP815
|
4.4
|
30.8
|
1.0
|
C
|
B:THR822
|
4.5
|
27.8
|
1.0
|
O
|
B:GLY821
|
4.5
|
33.9
|
1.0
|
CB
|
B:ARG823
|
4.5
|
43.9
|
1.0
|
CA
|
B:THR822
|
4.6
|
28.4
|
1.0
|
CG
|
B:ARG823
|
4.6
|
62.9
|
1.0
|
C
|
B:GLY821
|
4.6
|
34.1
|
1.0
|
N
|
B:THR822
|
4.6
|
30.4
|
1.0
|
C
|
B:LYS820
|
4.6
|
37.3
|
1.0
|
C
|
B:SER816
|
4.7
|
26.6
|
1.0
|
C
|
B:GLY819
|
4.8
|
38.4
|
1.0
|
NE
|
B:ARG823
|
4.8
|
86.3
|
1.0
|
CA
|
B:GLY819
|
4.8
|
39.8
|
1.0
|
CA
|
B:ARG823
|
4.8
|
31.1
|
1.0
|
N
|
B:GLY819
|
4.9
|
41.6
|
1.0
|
N
|
B:LYS820
|
4.9
|
36.1
|
1.0
|
|
Reference:
G.Gogl,
B.Biri-Kovacs,
A.L.Poti,
H.Vadaszi,
B.Szeder,
A.Bodor,
G.Schlosser,
A.Acs,
L.Turiak,
L.Buday,
A.Alexa,
L.Nyitray,
A.Remenyi.
Dynamic Control of Rsk Complexes By Phosphoswitch-Based Regulation. Febs J. V. 285 46 2018.
ISSN: ISSN 1742-4658
PubMed: 29083550
DOI: 10.1111/FEBS.14311
Page generated: Mon Jul 15 08:50:44 2024
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