Calcium in PDB 5n7g: Magi-1 Complexed with A Synthetic PRSK1 Peptide
Enzymatic activity of Magi-1 Complexed with A Synthetic PRSK1 Peptide
All present enzymatic activity of Magi-1 Complexed with A Synthetic PRSK1 Peptide:
2.7.11.1;
Protein crystallography data
The structure of Magi-1 Complexed with A Synthetic PRSK1 Peptide, PDB code: 5n7g
was solved by
G.Gogl,
L.Nyitray,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
Resolution Low / High (Å)
|
49.11 /
2.95
|
Space group
|
C 1 2 1
|
Cell size a, b, c (Å), α, β, γ (°)
|
194.930,
60.230,
99.350,
90.00,
98.69,
90.00
|
R / Rfree (%)
|
21 /
26.4
|
Calcium Binding Sites:
The binding sites of Calcium atom in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
(pdb code 5n7g). This binding sites where shown within
5.0 Angstroms radius around Calcium atom.
In total 9 binding sites of Calcium where determined in the
Magi-1 Complexed with A Synthetic PRSK1 Peptide, PDB code: 5n7g:
Jump to Calcium binding site number:
1;
2;
3;
4;
5;
6;
7;
8;
9;
Calcium binding site 1 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 1 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 1 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca904
b:44.5
occ:1.00
|
O
|
A:VAL590
|
2.5
|
44.9
|
1.0
|
O
|
A:GLY589
|
2.9
|
61.4
|
1.0
|
OE1
|
A:GLU592
|
3.0
|
24.1
|
1.0
|
C
|
A:GLY589
|
3.3
|
52.3
|
1.0
|
OE2
|
A:GLU592
|
3.3
|
22.5
|
1.0
|
CD
|
A:GLU592
|
3.5
|
18.2
|
1.0
|
C
|
A:VAL590
|
3.6
|
37.0
|
1.0
|
N
|
A:VAL590
|
3.8
|
21.7
|
1.0
|
CA
|
A:GLY589
|
4.0
|
36.0
|
1.0
|
CA
|
A:VAL590
|
4.2
|
21.1
|
1.0
|
N
|
A:ASP591
|
4.6
|
21.0
|
1.0
|
CB
|
A:VAL590
|
4.8
|
20.3
|
1.0
|
N
|
A:GLU592
|
4.9
|
18.9
|
1.0
|
CA
|
A:ASP591
|
4.9
|
39.4
|
1.0
|
CG
|
A:GLU592
|
4.9
|
17.4
|
1.0
|
|
Calcium binding site 2 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 2 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 2 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca905
b:34.1
occ:1.00
|
O
|
A:GLY746
|
2.4
|
36.5
|
1.0
|
O
|
A:ARG744
|
2.5
|
47.3
|
1.0
|
OE1
|
A:GLU786
|
2.5
|
52.8
|
1.0
|
OE2
|
A:GLU786
|
2.7
|
49.2
|
1.0
|
O
|
A:GLY741
|
2.8
|
37.5
|
1.0
|
CD
|
A:GLU786
|
2.9
|
43.3
|
1.0
|
C
|
A:GLY746
|
3.5
|
45.6
|
1.0
|
N
|
A:GLY746
|
3.5
|
39.2
|
1.0
|
C
|
A:GLY741
|
3.6
|
29.6
|
1.0
|
C
|
A:ARG744
|
3.7
|
28.8
|
1.0
|
CA
|
A:GLY746
|
4.0
|
48.5
|
1.0
|
C
|
A:LYS745
|
4.0
|
35.0
|
1.0
|
CA
|
A:LYS745
|
4.2
|
21.0
|
1.0
|
OG1
|
A:THR747
|
4.2
|
45.1
|
1.0
|
CA
|
A:VAL742
|
4.3
|
29.9
|
1.0
|
O
|
A:VAL742
|
4.3
|
52.6
|
1.0
|
N
|
A:VAL742
|
4.3
|
27.0
|
1.0
|
N
|
A:LYS745
|
4.4
|
20.6
|
1.0
|
C
|
A:VAL742
|
4.4
|
38.0
|
1.0
|
CA
|
A:GLY741
|
4.4
|
22.3
|
1.0
|
CG
|
A:GLU786
|
4.4
|
28.6
|
1.0
|
N
|
A:THR747
|
4.6
|
42.1
|
1.0
|
N
|
A:ARG744
|
4.7
|
34.3
|
1.0
|
CA
|
A:ARG744
|
4.8
|
29.3
|
1.0
|
CG
|
A:ARG744
|
4.9
|
18.6
|
1.0
|
O
|
A:LYS745
|
4.9
|
34.8
|
1.0
|
CA
|
A:THR747
|
5.0
|
37.5
|
1.0
|
|
Calcium binding site 3 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 3 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 3 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca906
b:35.0
occ:1.00
|
OG
|
A:SER773
|
2.5
|
24.0
|
1.0
|
CB
|
A:SER773
|
3.2
|
19.4
|
1.0
|
CA
|
A:SER773
|
3.2
|
14.2
|
1.0
|
O
|
A:SER773
|
3.3
|
12.3
|
1.0
|
C
|
A:SER773
|
3.7
|
14.0
|
1.0
|
N
|
A:SER773
|
4.5
|
20.8
|
1.0
|
O
|
A:LYS772
|
4.9
|
36.5
|
1.0
|
|
Calcium binding site 4 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 4 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 4 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca907
b:49.6
occ:1.00
|
NH2
|
A:ARG823
|
2.7
|
56.0
|
1.0
|
O
|
A:ASP815
|
3.2
|
30.0
|
1.0
|
N
|
A:GLY819
|
3.3
|
22.8
|
1.0
|
CZ
|
A:ARG823
|
3.5
|
68.6
|
1.0
|
CA
|
A:GLY819
|
3.6
|
37.9
|
1.0
|
C
|
A:SER816
|
3.9
|
9.1
|
1.0
|
C
|
A:GLY819
|
3.9
|
29.3
|
1.0
|
O
|
A:SER816
|
3.9
|
9.5
|
1.0
|
O
|
A:LYS820
|
4.0
|
21.4
|
1.0
|
NH1
|
A:ARG823
|
4.0
|
77.2
|
1.0
|
N
|
A:MET817
|
4.1
|
10.2
|
1.0
|
N
|
A:LYS818
|
4.2
|
22.7
|
1.0
|
CG
|
A:ARG823
|
4.2
|
44.2
|
1.0
|
O
|
A:GLY819
|
4.2
|
17.0
|
1.0
|
O
|
A:GLY821
|
4.3
|
19.3
|
1.0
|
NE
|
A:ARG823
|
4.3
|
75.5
|
1.0
|
C
|
A:MET817
|
4.3
|
10.1
|
1.0
|
C
|
A:ASP815
|
4.4
|
23.5
|
1.0
|
CA
|
A:SER816
|
4.4
|
8.7
|
1.0
|
CA
|
A:MET817
|
4.4
|
9.7
|
1.0
|
N
|
A:LYS820
|
4.5
|
20.6
|
1.0
|
C
|
A:LYS818
|
4.5
|
18.9
|
1.0
|
CD
|
A:ARG823
|
4.6
|
65.3
|
1.0
|
CA
|
A:CA909
|
4.7
|
34.1
|
1.0
|
C
|
A:LYS820
|
4.8
|
18.9
|
1.0
|
N
|
A:ARG823
|
4.8
|
10.4
|
1.0
|
C
|
A:GLY821
|
4.8
|
20.0
|
1.0
|
CG
|
A:LYS818
|
4.8
|
12.4
|
1.0
|
N
|
A:SER816
|
4.9
|
23.6
|
1.0
|
CA
|
A:LYS818
|
4.9
|
13.8
|
1.0
|
O
|
A:MET817
|
4.9
|
40.6
|
1.0
|
CA
|
A:THR822
|
5.0
|
10.9
|
1.0
|
|
Calcium binding site 5 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 5 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 5 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca908
b:76.7
occ:1.00
|
OD1
|
A:ASP524
|
2.9
|
64.2
|
1.0
|
CG
|
A:ASP524
|
4.0
|
60.3
|
1.0
|
CA
|
A:ASP524
|
4.2
|
25.5
|
1.0
|
OG
|
A:SER521
|
4.3
|
33.1
|
1.0
|
OE2
|
A:GLU551
|
4.6
|
60.8
|
1.0
|
C
|
A:ASP524
|
4.6
|
37.3
|
1.0
|
CB
|
A:ASP524
|
4.7
|
25.7
|
1.0
|
O
|
A:ASP524
|
4.7
|
31.7
|
1.0
|
CB
|
A:SER521
|
4.9
|
30.1
|
1.0
|
|
Calcium binding site 6 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 6 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 6 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
A:Ca909
b:34.1
occ:1.00
|
O
|
A:MET817
|
2.3
|
40.6
|
1.0
|
OD1
|
A:ASP861
|
2.4
|
19.0
|
1.0
|
O
|
A:GLY821
|
2.4
|
19.3
|
1.0
|
OD2
|
A:ASP861
|
2.6
|
35.5
|
1.0
|
O
|
A:GLY819
|
2.8
|
17.0
|
1.0
|
CG
|
A:ASP861
|
2.8
|
21.5
|
1.0
|
C
|
A:MET817
|
3.0
|
10.1
|
1.0
|
CA
|
A:MET817
|
3.3
|
9.7
|
1.0
|
C
|
A:GLY821
|
3.6
|
20.0
|
1.0
|
C
|
A:GLY819
|
3.9
|
29.3
|
1.0
|
N
|
A:LYS818
|
4.1
|
22.7
|
1.0
|
CB
|
A:MET817
|
4.1
|
9.6
|
1.0
|
N
|
A:GLY819
|
4.2
|
22.8
|
1.0
|
OG1
|
A:THR822
|
4.2
|
27.2
|
1.0
|
C
|
A:LYS818
|
4.2
|
18.9
|
1.0
|
CB
|
A:ASP861
|
4.3
|
16.8
|
1.0
|
N
|
A:GLY821
|
4.3
|
14.5
|
1.0
|
N
|
A:THR822
|
4.5
|
26.7
|
1.0
|
CA
|
A:GLY821
|
4.5
|
12.9
|
1.0
|
C
|
A:LYS820
|
4.6
|
18.9
|
1.0
|
N
|
A:MET817
|
4.6
|
10.2
|
1.0
|
CA
|
A:THR822
|
4.6
|
10.9
|
1.0
|
CA
|
A:LYS818
|
4.6
|
13.8
|
1.0
|
O
|
A:LYS818
|
4.6
|
24.4
|
1.0
|
CA
|
A:GLY819
|
4.6
|
37.9
|
1.0
|
O
|
A:SER816
|
4.7
|
9.5
|
1.0
|
CA
|
A:CA907
|
4.7
|
49.6
|
1.0
|
CG
|
A:MET817
|
4.8
|
9.4
|
1.0
|
N
|
A:LYS820
|
4.9
|
20.6
|
1.0
|
CB
|
A:THR822
|
4.9
|
29.7
|
1.0
|
O
|
A:LYS820
|
4.9
|
21.4
|
1.0
|
CA
|
A:LYS820
|
5.0
|
18.6
|
1.0
|
|
Calcium binding site 7 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 7 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 7 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca903
b:89.6
occ:1.00
|
OE2
|
B:GLU786
|
2.8
|
0.3
|
1.0
|
OE1
|
B:GLU786
|
3.3
|
0.8
|
1.0
|
CD
|
B:GLU786
|
3.4
|
0.8
|
1.0
|
NZ
|
B:LYS745
|
3.9
|
88.1
|
1.0
|
O
|
B:VAL742
|
4.2
|
0.8
|
1.0
|
CA
|
B:VAL742
|
4.4
|
95.9
|
1.0
|
O
|
B:ARG744
|
4.5
|
98.2
|
1.0
|
CG2
|
B:VAL742
|
4.5
|
94.8
|
1.0
|
CD
|
B:LYS745
|
4.7
|
98.1
|
1.0
|
O
|
B:GLY741
|
4.7
|
78.5
|
1.0
|
CE
|
B:LYS745
|
4.7
|
95.2
|
1.0
|
C
|
B:VAL742
|
4.8
|
0.7
|
1.0
|
CG
|
B:GLU786
|
4.8
|
0.2
|
1.0
|
N
|
B:GLY746
|
5.0
|
96.8
|
1.0
|
CB
|
B:VAL742
|
5.0
|
95.9
|
1.0
|
|
Calcium binding site 8 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 8 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 8 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca904
b:90.3
occ:1.00
|
O
|
B:VAL590
|
3.1
|
58.9
|
1.0
|
N
|
B:VAL590
|
3.6
|
52.3
|
1.0
|
C
|
B:GLY589
|
3.7
|
56.0
|
1.0
|
O
|
B:GLY589
|
3.9
|
70.3
|
1.0
|
C
|
B:VAL590
|
4.0
|
42.4
|
1.0
|
CA
|
B:GLY589
|
4.2
|
48.9
|
1.0
|
CD
|
B:GLU592
|
4.3
|
69.0
|
1.0
|
CA
|
B:VAL590
|
4.3
|
47.8
|
1.0
|
CG
|
B:GLU592
|
4.4
|
56.4
|
1.0
|
OE2
|
B:GLU592
|
4.4
|
77.5
|
1.0
|
OE1
|
B:GLU592
|
4.6
|
69.7
|
1.0
|
CB
|
B:VAL590
|
4.7
|
33.4
|
1.0
|
|
Calcium binding site 9 out
of 9 in 5n7g
Go back to
Calcium Binding Sites List in 5n7g
Calcium binding site 9 out
of 9 in the Magi-1 Complexed with A Synthetic PRSK1 Peptide
Mono view
Stereo pair view
|
A full contact list of Calcium with other atoms in the Ca binding
site number 9 of Magi-1 Complexed with A Synthetic PRSK1 Peptide within 5.0Å range:
probe
|
atom
|
residue
|
distance (Å)
|
B
|
Occ
|
B:Ca905
b:38.0
occ:1.00
|
O
|
B:GLY821
|
2.5
|
38.1
|
1.0
|
OD1
|
B:ASP861
|
2.5
|
37.5
|
1.0
|
OD2
|
B:ASP861
|
2.5
|
51.8
|
1.0
|
O
|
B:GLY819
|
2.6
|
57.0
|
1.0
|
O
|
B:MET817
|
2.8
|
44.4
|
1.0
|
CG
|
B:ASP861
|
2.9
|
55.3
|
1.0
|
C
|
B:GLY821
|
3.6
|
45.2
|
1.0
|
C
|
B:MET817
|
3.7
|
42.5
|
1.0
|
C
|
B:GLY819
|
3.8
|
60.4
|
1.0
|
N
|
B:GLY821
|
3.9
|
47.4
|
1.0
|
CA
|
B:MET817
|
4.1
|
42.3
|
1.0
|
C
|
B:LYS820
|
4.2
|
55.6
|
1.0
|
CA
|
B:GLY821
|
4.2
|
37.9
|
1.0
|
OG1
|
B:THR822
|
4.3
|
36.2
|
1.0
|
CB
|
B:ASP861
|
4.4
|
36.5
|
1.0
|
N
|
B:GLY819
|
4.5
|
43.1
|
1.0
|
CA
|
B:LYS820
|
4.6
|
53.9
|
1.0
|
CB
|
B:MET817
|
4.6
|
40.8
|
1.0
|
N
|
B:LYS820
|
4.6
|
58.1
|
1.0
|
N
|
B:THR822
|
4.7
|
37.0
|
1.0
|
CA
|
B:GLY819
|
4.7
|
58.7
|
1.0
|
C
|
B:LYS818
|
4.8
|
47.8
|
1.0
|
N
|
B:LYS818
|
4.8
|
44.6
|
1.0
|
O
|
B:LYS820
|
4.8
|
52.3
|
1.0
|
CA
|
B:THR822
|
5.0
|
36.5
|
1.0
|
|
Reference:
G.Gogl,
B.Biri-Kovacs,
A.L.Poti,
H.Vadaszi,
B.Szeder,
A.Bodor,
G.Schlosser,
A.Acs,
L.Turiak,
L.Buday,
A.Alexa,
L.Nyitray,
A.Remenyi.
Dynamic Control of Rsk Complexes By Phosphoswitch-Based Regulation. Febs J. V. 285 46 2018.
ISSN: ISSN 1742-4658
PubMed: 29083550
DOI: 10.1111/FEBS.14311
Page generated: Mon Jul 15 08:51:12 2024
|