Atomistry » Calcium » PDB 5s5u-5sy1 » 5sic
Atomistry »
  Calcium »
    PDB 5s5u-5sy1 »
      5sic »

Calcium in PDB 5sic: Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor)

Enzymatic activity of Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor)

All present enzymatic activity of Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor):
3.4.21.62;

Protein crystallography data

The structure of Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor), PDB code: 5sic was solved by Y.Mitsui, Y.Takeuchi, K.T.Nakamura, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) N/A / 2.20
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 77.200, 185.900, 69.500, 90.00, 90.00, 90.00
R / Rfree (%) n/a / n/a

Calcium Binding Sites:

The binding sites of Calcium atom in the Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor) (pdb code 5sic). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor), PDB code: 5sic:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in 5sic

Go back to Calcium Binding Sites List in 5sic
Calcium binding site 1 out of 2 in the Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor) within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Ca501

b:4.1
occ:1.00
OD1 E:ASP41 2.1 3.1 1.0
O E:VAL81 2.2 4.6 1.0
O E:LEU75 2.3 2.6 1.0
OD1 E:ASN77 2.4 2.0 1.0
O E:ILE79 2.4 4.2 1.0
OD2 E:ASP41 2.5 2.1 1.0
OE1 E:GLN2 2.6 4.0 1.0
CG E:ASP41 2.7 2.6 1.0
C E:LEU75 3.3 3.3 1.0
CG E:ASN77 3.4 2.0 1.0
C E:VAL81 3.4 4.5 1.0
CD E:GLN2 3.6 4.8 1.0
C E:ILE79 3.6 4.2 1.0
N E:VAL81 3.8 5.8 1.0
ND2 E:ASN77 4.0 2.0 1.0
N E:ASN77 4.0 4.1 1.0
N E:ASN76 4.1 5.2 1.0
CB E:ASP41 4.1 2.0 1.0
C E:GLY80 4.1 5.9 1.0
CG E:GLN2 4.2 5.3 1.0
CA E:LEU75 4.2 2.0 1.0
CA E:GLY80 4.2 4.7 1.0
CA E:ASN76 4.2 4.8 1.0
N E:LEU75 4.3 2.0 1.0
CA E:VAL81 4.3 4.2 1.0
N E:GLY80 4.3 5.5 1.0
N E:LEU82 4.4 4.3 1.0
CB E:LEU75 4.4 2.1 1.0
C E:ALA74 4.5 2.0 1.0
CA E:LEU82 4.7 3.3 1.0
C E:ASN76 4.7 6.6 1.0
N E:ILE79 4.7 4.4 1.0
CB E:ASN77 4.7 2.0 1.0
NE2 E:GLN2 4.7 5.0 1.0
CA E:ILE79 4.8 3.5 1.0
CA E:ASN77 4.8 2.0 1.0
O E:ALA74 4.8 2.0 1.0
CG1 E:ILE79 4.8 2.0 1.0
CA E:ASP41 4.9 2.0 1.0
CB E:GLN2 4.9 6.2 1.0
O E:GLY80 4.9 6.6 1.0
C E:ASN77 5.0 2.3 1.0
O E:ASP41 5.0 2.3 1.0

Calcium binding site 2 out of 2 in 5sic

Go back to Calcium Binding Sites List in 5sic
Calcium binding site 2 out of 2 in the Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor)


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor) within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Ca502

b:26.8
occ:1.00
O E:HOH631 2.5 10.7 1.0
O E:HOH632 2.8 13.3 1.0
O E:GLU195 2.9 8.8 1.0
O E:TYR171 2.9 9.2 1.0
O E:GLY169 2.9 8.1 1.0
O E:VAL174 3.0 10.6 1.0
OD2 E:ASP197 3.1 9.4 1.0
C E:TYR171 3.6 7.8 1.0
O E:LYS170 3.7 9.9 1.0
CA E:PRO172 3.7 7.6 1.0
OD1 E:ASP197 4.0 8.6 1.0
C E:GLU195 4.0 9.4 1.0
CG E:ASP197 4.0 8.9 1.0
N E:PRO172 4.0 8.4 1.0
C E:GLY169 4.0 9.4 1.0
C E:LYS170 4.0 9.4 1.0
CB E:ALA176 4.1 6.8 1.0
C E:VAL174 4.2 10.0 1.0
CB E:GLU195 4.2 12.8 1.0
C E:PRO172 4.2 7.7 1.0
CA E:GLU195 4.4 10.8 1.0
O E:PRO172 4.4 9.4 1.0
N E:ALA176 4.5 7.0 1.0
N E:TYR171 4.5 7.8 1.0
CA E:LYS170 4.6 8.9 1.0
N E:VAL174 4.7 11.0 1.0
CA E:TYR171 4.7 7.5 1.0
NH2 E:ARG247 4.7 2.0 1.0
N E:LYS170 4.7 8.4 1.0
CA E:VAL174 4.9 10.1 1.0
N E:SER173 4.9 8.0 1.0
CA E:GLY169 4.9 10.0 1.0

Reference:

Y.Takeuchi, S.Noguchi, Y.Satow, S.Kojima, I.Kumagai, K.Miura, K.T.Nakamura, Y.Mitsui. Molecular Recognition at the Active Site of Subtilisin Bpn': Crystallographic Studies Using Genetically Engineered Proteinaceous Inhibitor Ssi (Streptomyces Subtilisin Inhibitor). Protein Eng. V. 4 501 1991.
ISSN: ISSN 0269-2139
PubMed: 1891457
DOI: 10.1093/PROTEIN/4.5.501
Page generated: Mon Jul 15 10:33:17 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy