Calcium in PDB 5ssy: Crystal Structure of Human Formylglycine Generating Enzyme

All present enzymatic activity of Crystal Structure of Human Formylglycine Generating Enzyme:
1.8.3.7;

The structure of Crystal Structure of Human Formylglycine Generating Enzyme, PDB code: 5ssy was solved by K.Radhakrishnan, L.Schlotawa, M.G.Rudolph, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	54.86 / 1.29
Space group	P 2 21 21
Cell size a, b, c (Å), α, β, γ (°)	43.475, 61.731, 109.719, 90, 90, 90
R / Rfree (%)	21.2 / 23.7

The structure of Crystal Structure of Human Formylglycine Generating Enzyme also contains other interesting chemical elements:

Copper

(Cu)

1 atom

The binding sites of Calcium atom in the Crystal Structure of Human Formylglycine Generating Enzyme (pdb code 5ssy). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Crystal Structure of Human Formylglycine Generating Enzyme, PDB code: 5ssy:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Crystal Structure of Human Formylglycine Generating Enzyme


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Crystal Structure of Human Formylglycine Generating Enzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca403 b:7.6 occ:1.00 OE2 A:GLU300 2.3 7.2 1.0 O A:ALA298 2.3 8.1 1.0 OE2 A:GLU130 2.3 7.4 1.0 O A:ASN293 2.4 7.3 1.0 O A:HOH597 2.4 9.2 1.0 O A:GLY296 2.4 6.4 1.0 CD A:GLU300 3.3 5.8 1.0 CD A:GLU130 3.4 9.3 1.0 C A:ALA298 3.5 7.4 1.0 C A:ASN293 3.6 9.8 1.0 CG A:GLU300 3.6 10.6 1.0 C A:GLY296 3.6 7.3 1.0 N A:ALA298 3.7 8.3 1.0 O A:ILE294 3.8 9.6 1.0 CG A:GLU130 3.9 9.2 1.0 C A:ILE294 4.0 9.0 1.0 CA A:ALA298 4.1 5.2 1.0 CB A:ASN297 4.3 11.1 1.0 CA A:ILE294 4.3 9.5 0.5 O A:GLY332 4.3 10.7 1.0 N A:GLY296 4.4 7.6 1.0 CA A:ILE294 4.4 9.5 0.5 CB A:ASN293 4.4 9.1 1.0 C A:VAL295 4.4 8.6 1.0 OE1 A:GLU130 4.4 9.6 1.0 NH2 A:ARG364 4.4 7.5 1.0 N A:ILE294 4.4 8.9 1.0 OE1 A:GLU300 4.4 8.0 1.0 C A:ASN297 4.5 8.0 1.0 CA A:ASN293 4.6 7.2 1.0 N A:ASN297 4.6 7.0 1.0 N A:TRP299 4.6 7.9 1.0 CA A:GLY296 4.6 7.0 1.0 N A:VAL295 4.6 7.2 1.0 O A:VAL295 4.6 10.2 1.0 CA A:ASN297 4.6 10.1 1.0 CB A:ALA298 4.7 7.3 1.0 CA A:VAL295 4.8 7.9 1.0 CA A:TRP299 4.9 7.4 1.0 C A:TRP299 5.0 6.3 1.0

Calcium binding site 2 out of 2 in the Crystal Structure of Human Formylglycine Generating Enzyme


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Crystal Structure of Human Formylglycine Generating Enzyme within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca404 b:7.8 occ:1.00 O A:PHE275 2.3 9.2 1.0 O A:ILE260 2.3 8.2 1.0 O A:HOH556 2.4 7.7 1.0 OD1 A:ASN259 2.4 7.3 1.0 O A:HOH590 2.4 9.1 1.0 OD1 A:ASP273 2.4 9.7 1.0 OD2 A:ASP273 2.5 8.8 1.0 CG A:ASP273 2.8 9.7 1.0 C A:ILE260 3.5 8.8 1.0 C A:PHE275 3.5 9.5 1.0 CG A:ASN259 3.6 8.1 1.0 N A:ILE260 3.8 7.3 1.0 OE1 A:GLN262 4.0 8.8 1.0 C A:ASN259 4.2 8.0 1.0 CA A:ILE260 4.2 8.3 1.0 CB A:ASP273 4.3 6.5 1.0 CA A:ASN259 4.3 8.9 1.0 CA A:PHE275 4.3 8.9 1.0 NE2 A:GLN262 4.4 10.0 1.0 N A:PHE275 4.4 10.2 1.0 CB A:PHE275 4.5 7.8 1.0 ND2 A:ASN259 4.5 7.5 1.0 N A:GLN276 4.5 8.2 1.0 N A:TRP261 4.5 9.7 1.0 CB A:ASN259 4.6 9.1 1.0 CA A:GLN276 4.6 9.8 1.0 O A:GLN276 4.6 9.7 1.0 O A:TYR334 4.6 10.9 1.0 CD A:GLN262 4.6 9.3 1.0 OD1 A:ASN269 4.6 12.8 1.0 CA A:TRP261 4.7 9.0 1.0 ND2 A:ASN269 4.7 9.9 1.0 C A:GLN276 4.8 9.5 1.0 O A:GLY277 4.9 9.4 1.0 CB A:TYR334 4.9 9.8 1.0 O A:ASN259 5.0 8.7 1.0

J.Kowal, L.Schlotawa, M.G.Rudolph, H.Niemann. Crystal Structure of Human Formylglycine Generating Enzyme in Complex with N-Acetyl-Cysteine Methylester To Be Published.