Calcium in PDB 5t5n: Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium

Protein crystallography data

The structure of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium, PDB code: 5t5n was solved by S.B.Long, G.Vaisey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.95 / 3.10
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 98.243, 243.878, 172.052, 90.00, 93.84, 90.00
R / Rfree (%) 21.8 / 24.3

Other elements in 5t5n:

The structure of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium also contains other interesting chemical elements:

Potassium (K) 5 atoms
Chlorine (Cl) 15 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium (pdb code 5t5n). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 5 binding sites of Calcium where determined in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium, PDB code: 5t5n:
Jump to Calcium binding site number: 1; 2; 3; 4; 5;

Calcium binding site 1 out of 5 in 5t5n

Go back to Calcium Binding Sites List in 5t5n
Calcium binding site 1 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Ca505

b:70.1
occ:1.00
 O E:ALA10 2.3 89.8 1.0
O A:HOH601 2.3 91.8 1.0
OD2 A:ASP301 2.3 90.9 1.0
O A:ASN296 2.5 79.4 1.0
OD2 A:ASP304 2.6 83.7 1.0
O A:GLN293 2.7 85.7 1.0
OD1 A:ASP304 3.0 68.8 1.0
CG A:ASP304 3.2 78.3 1.0
C E:ALA10 3.4 82.0 1.0
C A:ASN296 3.5 74.7 1.0
CG A:ASP301 3.5 77.9 1.0
C A:GLN293 3.8 79.0 1.0
CB A:ASP301 4.1 83.8 1.0
CA E:ASP11 4.2 94.6 1.0
N E:ASP11 4.2 90.1 1.0
CB A:ASN296 4.2 82.6 1.0
CA A:ASN296 4.2 89.4 1.0
N A:ASN296 4.3 78.4 1.0
CA A:GLN293 4.3 52.8 1.0
O E:VAL9 4.3 85.4 1.0
CA E:ALA10 4.4 87.7 1.0
CA A:GLY299 4.4 72.7 1.0
N A:GLY299 4.5 82.1 1.0
N A:PRO297 4.5 75.6 1.0
OD1 A:ASP301 4.5 0.0 1.0
CA A:PRO297 4.6 74.4 1.0
O A:GLU292 4.6 62.0 1.0
CB A:ASP304 4.6 82.2 1.0
CG A:GLN293 4.7 73.8 1.0
C E:ASP11 4.7 84.2 1.0
N A:ASP301 4.8 82.1 1.0
N E:ALA12 4.8 72.0 1.0
OD1 E:ASP11 4.9 0.2 1.0
N A:LEU294 4.9 76.6 1.0
CG A:ASN296 4.9 70.9 1.0
OD1 A:ASN296 5.0 95.5 1.0

Calcium binding site 2 out of 5 in 5t5n

Go back to Calcium Binding Sites List in 5t5n
Calcium binding site 2 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Ca502

b:79.7
occ:1.00
 O A:ALA10 2.4 78.8 1.0
OD2 B:ASP301 2.4 74.6 1.0
O B:ASN296 2.4 79.5 1.0
O B:HOH601 2.5 70.3 1.0
OD2 B:ASP304 2.5 78.3 1.0
O B:GLN293 2.6 84.1 1.0
OD1 B:ASP304 2.8 73.2 1.0
CG B:ASP304 3.0 71.5 1.0
C B:ASN296 3.5 83.2 1.0
C A:ALA10 3.5 76.5 1.0
CG B:ASP301 3.6 78.9 1.0
C B:GLN293 3.7 74.1 1.0
CB B:ASP301 4.1 86.1 1.0
CA A:ASP11 4.2 82.1 1.0
CA B:ASN296 4.2 80.0 1.0
N B:ASN296 4.2 85.6 1.0
CA B:GLN293 4.2 49.7 1.0
N A:ASP11 4.3 82.4 1.0
CB B:ASN296 4.3 75.7 1.0
O A:VAL9 4.4 78.0 1.0
N B:PRO297 4.4 76.0 1.0
N B:GLY299 4.4 75.8 1.0
CA A:ALA10 4.5 75.1 1.0
CA B:GLY299 4.5 72.8 1.0
CA B:PRO297 4.5 69.1 1.0
CB B:ASP304 4.5 82.0 1.0
OD1 B:ASP301 4.6 85.7 1.0
O B:GLU292 4.6 73.0 1.0
CG B:GLN293 4.7 58.2 1.0
C A:ASP11 4.8 90.7 1.0
N B:LEU294 4.8 73.3 1.0
N B:ASP301 4.8 97.2 1.0
N A:ALA12 4.9 81.0 1.0
OD1 A:ASP11 4.9 96.5 1.0
C B:PRO297 5.0 67.6 1.0

Calcium binding site 3 out of 5 in 5t5n

Go back to Calcium Binding Sites List in 5t5n
Calcium binding site 3 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 3 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
C:Ca502

b:67.2
occ:1.00
 OD2 C:ASP301 2.4 79.0 1.0
O B:ALA10 2.4 71.3 1.0
O C:ASN296 2.4 73.7 1.0
OD2 C:ASP304 2.5 77.0 1.0
O C:HOH601 2.5 82.7 1.0
O C:GLN293 2.7 81.3 1.0
OD1 C:ASP304 2.8 82.0 1.0
CG C:ASP304 3.0 74.9 1.0
C C:ASN296 3.5 77.8 1.0
C B:ALA10 3.5 71.4 1.0
CG C:ASP301 3.5 78.8 1.0
C C:GLN293 3.8 76.3 1.0
CB C:ASP301 4.1 77.7 1.0
CA B:ASP11 4.2 89.6 1.0
CA C:ASN296 4.3 75.0 1.0
N B:ASP11 4.3 92.3 1.0
CB C:ASN296 4.3 71.4 1.0
CA C:GLN293 4.3 52.7 1.0
N C:ASN296 4.3 73.9 1.0
N C:GLY299 4.4 76.0 1.0
N C:PRO297 4.4 80.1 1.0
CA C:GLY299 4.4 67.8 1.0
O B:VAL9 4.4 91.7 1.0
CA B:ALA10 4.5 92.2 1.0
CB C:ASP304 4.5 61.0 1.0
CA C:PRO297 4.5 70.4 1.0
OD1 C:ASP301 4.6 92.0 1.0
CG C:GLN293 4.6 55.8 1.0
O C:GLU292 4.7 70.4 1.0
N C:ASP301 4.8 69.4 1.0
C B:ASP11 4.8 77.2 1.0
N B:ALA12 4.9 74.0 1.0
OD1 B:ASP11 4.9 80.3 1.0
N C:LEU294 4.9 73.3 1.0
C C:PRO297 4.9 69.5 1.0
CG C:ASN296 5.0 77.1 1.0
C C:GLY299 5.0 75.4 1.0

Calcium binding site 4 out of 5 in 5t5n

Go back to Calcium Binding Sites List in 5t5n
Calcium binding site 4 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 4 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Ca502

b:53.5
occ:1.00
 OD2 D:ASP301 2.4 74.5 1.0
O D:ASN296 2.4 79.7 1.0
O C:ALA10 2.5 69.2 1.0
O D:HOH601 2.5 69.4 1.0
O D:GLN293 2.6 84.5 1.0
OD2 D:ASP304 2.6 66.2 1.0
OD1 D:ASP304 2.8 70.7 1.0
CG D:ASP304 3.1 70.6 1.0
C D:ASN296 3.5 64.4 1.0
C C:ALA10 3.6 60.3 1.0
CG D:ASP301 3.6 66.1 1.0
C D:GLN293 3.7 68.2 1.0
CB D:ASP301 4.1 74.5 1.0
CA D:GLN293 4.2 52.5 1.0
CA D:ASN296 4.2 64.4 1.0
N D:ASN296 4.2 65.9 1.0
CA C:ASP11 4.3 73.8 1.0
CB D:ASN296 4.3 64.0 1.0
N C:ASP11 4.3 86.0 1.0
O C:VAL9 4.4 69.0 1.0
N D:PRO297 4.4 69.5 1.0
CA D:PRO297 4.5 60.9 1.0
N D:GLY299 4.5 76.5 1.0
CA C:ALA10 4.5 61.6 1.0
CA D:GLY299 4.6 64.1 1.0
CB D:ASP304 4.6 75.2 1.0
CG D:GLN293 4.6 62.1 1.0
OD1 D:ASP301 4.6 76.5 1.0
O D:GLU292 4.6 55.4 1.0
N D:LEU294 4.8 55.5 1.0
C C:ASP11 4.8 68.2 1.0
N C:ALA12 4.9 73.5 1.0
N D:ASP301 4.9 63.0 1.0
C D:PRO297 5.0 68.7 1.0

Calcium binding site 5 out of 5 in 5t5n

Go back to Calcium Binding Sites List in 5t5n
Calcium binding site 5 out of 5 in the Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 5 of Calcium-Activated Chloride Channel Bestrophin-1 (BEST1), Triple Mutant: I76A, F80A, F84A; in Complex with An Fab Antibody Fragment, Chloride, and Calcium within 5.0Å range:
probe atom residue distance (Å) B Occ
E:Ca502

b:49.1
occ:1.00
 OD2 E:ASP301 2.4 84.5 1.0
O D:ALA10 2.4 62.7 1.0
O E:ASN296 2.4 96.3 1.0
OD2 E:ASP304 2.5 77.0 1.0
O E:HOH601 2.5 88.7 1.0
O E:GLN293 2.6 78.4 1.0
OD1 E:ASP304 2.7 84.0 1.0
CG E:ASP304 3.0 77.7 1.0
C D:ALA10 3.5 67.7 1.0
CG E:ASP301 3.5 68.7 1.0
C E:ASN296 3.5 69.9 1.0
C E:GLN293 3.7 71.3 1.0
CB E:ASP301 4.0 73.0 1.0
CA D:ASP11 4.2 76.2 1.0
CA E:GLN293 4.2 44.9 1.0
N D:ASP11 4.2 70.4 1.0
CA E:ASN296 4.3 58.5 1.0
CB E:ASN296 4.3 57.4 1.0
N E:ASN296 4.3 47.9 1.0
O D:VAL9 4.4 73.8 1.0
N E:GLY299 4.4 60.8 1.0
N E:PRO297 4.4 56.8 1.0
CA E:GLY299 4.4 65.2 1.0
CA D:ALA10 4.5 73.8 1.0
CB E:ASP304 4.5 67.1 1.0
CA E:PRO297 4.5 53.0 1.0
OD1 E:ASP301 4.6 85.5 1.0
CG E:GLN293 4.6 57.4 1.0
O E:GLU292 4.7 49.6 1.0
N E:ASP301 4.7 69.7 1.0
C D:ASP11 4.8 58.2 1.0
N D:ALA12 4.8 71.0 1.0
N E:LEU294 4.9 60.0 1.0
OD1 D:ASP11 4.9 83.0 1.0
C E:PRO297 5.0 64.6 1.0
CG E:ASN296 5.0 56.0 1.0

Reference:

G.Vaisey, A.N.Miller, S.B.Long. Distinct Regions That Control Ion Selectivity and Calcium-Dependent Activation in the Bestrophin Ion Channel. Proc. Natl. Acad. Sci. V. 113 E7399 2016U.S.A..
ISSN: ESSN 1091-6490
PubMed: 27821745
DOI: 10.1073/PNAS.1614688113
Page generated: Sat Dec 12 05:43:40 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy