Calcium in PDB 6gqg: Structure of GFPMUT2 Crystallized at pH 8.5

The structure of Structure of GFPMUT2 Crystallized at pH 8.5, PDB code: 6gqg was solved by E.Pasqualetto, G.Lolli, R.Battistutta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	46.50 / 1.79
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	51.166, 62.375, 69.767, 90.00, 90.00, 90.00
R / Rfree (%)	18.3 / 22.3

The binding sites of Calcium atom in the Structure of GFPMUT2 Crystallized at pH 8.5 (pdb code 6gqg). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Structure of GFPMUT2 Crystallized at pH 8.5, PDB code: 6gqg:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Structure of GFPMUT2 Crystallized at pH 8.5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Structure of GFPMUT2 Crystallized at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca301 b:33.6 occ:1.00 O A:HOH401 2.3 14.6 1.0 OD1 A:ASP197 2.7 29.7 1.0 O A:HOH592 2.8 35.1 1.0 OD2 A:ASP197 2.9 34.7 1.0 O A:HOH439 2.9 30.0 1.0 CG A:ASP197 3.2 23.2 1.0 CB A:ASP197 4.7 27.8 1.0 O A:HOH538 4.9 34.0 1.0

Calcium binding site 2 out of 2 in the Structure of GFPMUT2 Crystallized at pH 8.5


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Structure of GFPMUT2 Crystallized at pH 8.5 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca302 b:59.4 occ:1.00 O A:HOH609 2.5 44.2 1.0 O A:HIS139 2.5 22.0 1.0 O A:HOH510 2.6 51.1 1.0 O A:HOH405 2.7 35.9 1.0 OE1 A:GLU172 2.7 34.0 1.0 OE2 A:GLU172 2.8 38.0 1.0 CD A:GLU172 3.1 38.8 1.0 C A:HIS139 3.7 22.8 1.0 CA A:LYS140 4.3 17.2 1.0 O A:HOH413 4.3 41.7 1.0 N A:LYS140 4.5 19.9 1.0 O A:GLY138 4.6 19.4 1.0 CG A:GLU172 4.6 37.0 1.0 CB A:HIS139 4.7 19.5 1.0 CA A:HIS139 4.8 20.5 1.0

G.Lolli, S.Raboni, E.Pasqualetto, R.Benoni, B.Campanini, L.Ronda, A.Mozzarelli, S.Bettati, R.Battistutta. Insight Into GFPMUT2 pH Dependence By Single Crystal Microspectrophotometry and X-Ray Crystallography. J.Phys.Chem.B V. 122 11326 2018.
ISSN: ISSN 1089-5647
PubMed: 30179482
DOI: 10.1021/ACS.JPCB.8B07260