Calcium in PDB 7bve: Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol

All present enzymatic activity of Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol:
2.4.2.34;

The binding sites of Calcium atom in the Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol (pdb code 7bve). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol, PDB code: 7bve:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca1101 b:0.2 occ:1.00 OD2 A:ASP929 2.7 62.2 1.0 O A:HIS936 3.2 52.2 1.0 O A:ALA933 3.5 61.5 1.0 O A:ASP931 3.5 60.5 1.0 CG A:ASP929 3.7 62.2 1.0 CG A:GLN841 4.2 65.1 1.0 OD1 A:ASP929 4.2 62.2 1.0 C A:HIS936 4.2 52.2 1.0 C A:ALA933 4.3 61.5 1.0 CB A:GLN841 4.4 65.1 1.0 C A:ASP931 4.5 60.5 1.0 O A:SER835 4.5 49.1 1.0 CB A:ASP929 4.7 62.2 1.0 OG A:SER835 4.8 49.1 1.0 O A:PRO934 4.9 61.4 1.0 CA A:PRO934 4.9 61.4 1.0 CA A:ASP931 4.9 60.5 1.0 CB A:HIS936 4.9 52.2 1.0 N A:PRO934 5.0 61.4 1.0 NH1 A:ARG837 5.0 63.7 1.0

Calcium binding site 2 out of 2 in the Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Cryo-Em Structure of Mycobacterium Smegmatis Arabinosyltransferase EMBC2-ACPM2 in Complex with Ethambutol within 5.0Å range: probe atom residue distance (Å) B Occ B:Ca1101 b:0.3 occ:1.00 OD2 B:ASP929 2.7 62.0 1.0 O B:ASP931 2.9 60.7 1.0 O B:HIS936 3.1 53.2 1.0 CG B:ASP929 3.2 62.0 1.0 OD1 B:ASP929 3.4 62.0 1.0 CA B:ASP931 3.5 60.7 1.0 O B:ALA933 3.5 30.0 1.0 C B:ASP931 3.5 60.7 1.0 N B:ASP931 3.6 60.7 1.0 C B:HIS936 4.3 53.2 1.0 OD1 B:ASP931 4.4 60.7 1.0 CB B:ASP929 4.4 62.0 1.0 CD2 B:HIS936 4.5 53.2 1.0 C B:ASP930 4.6 66.2 1.0 O B:SER835 4.7 48.9 1.0 C B:ALA933 4.7 30.0 1.0 OG B:SER835 4.7 48.9 1.0 N B:LEU932 4.8 67.1 1.0 CB B:ASP931 4.8 60.7 1.0 N B:ASP930 4.8 66.2 1.0 CG B:GLN841 4.9 65.0 1.0 CB B:HIS936 4.9 53.2 1.0 CB B:GLN841 5.0 65.0 1.0

L.Zhang, Y.Zhao, Y.Gao, L.Wu, R.Gao, Q.Zhang, Y.Wang, C.Wu, F.Wu, S.S.Gurcha, N.Veerapen, S.M.Batt, W.Zhao, L.Qin, X.Yang, M.Wang, Y.Zhu, B.Zhang, L.Bi, X.Zhang, H.Yang, L.W.Guddat, W.Xu, Q.Wang, J.Li, G.S.Besra, Z.Rao. Structures of Cell Wall Arabinosyltransferases with the Anti-Tuberculosis Drug Ethambutol Science 2020.
ISSN: ESSN 1095-9203
DOI: 10.1126/SCIENCE.ABA9102