Calcium in PDB 8elk: Hras R97F Crystal Form 1

All present enzymatic activity of Hras R97F Crystal Form 1:
3.6.5.2;

The structure of Hras R97F Crystal Form 1, PDB code: 8elk was solved by C.W.Johnson, C.Mattos, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	44.07 / 1.80
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	88.147, 88.147, 133.27, 90, 90, 120
R / Rfree (%)	17 / 19.5

The structure of Hras R97F Crystal Form 1 also contains other interesting chemical elements:

Sodium	(Na)	1 atom
Magnesium	(Mg)	2 atoms

The binding sites of Calcium atom in the Hras R97F Crystal Form 1 (pdb code 8elk). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total 2 binding sites of Calcium where determined in the Hras R97F Crystal Form 1, PDB code: 8elk:
Jump to Calcium binding site number: 1; 2;

Calcium binding site 1 out of 2 in the Hras R97F Crystal Form 1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Hras R97F Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca203 b:19.0 occ:1.00 O A:PHE28 2.3 18.5 1.0 OD2 A:ASP30 2.4 22.8 1.0 C A:PHE28 3.6 18.8 1.0 CG A:ASP30 3.6 27.9 1.0 N A:ASP30 4.0 17.0 1.0 O A:HOH383 4.3 39.9 1.0 CA A:VAL29 4.3 17.5 1.0 N A:VAL29 4.4 15.7 1.0 CB A:ASP30 4.4 21.6 1.0 O A:HOH394 4.5 29.0 1.0 OD1 A:ASP30 4.5 26.2 1.0 CA A:PHE28 4.5 16.1 1.0 C A:VAL29 4.6 20.8 1.0 N A:PHE28 4.7 17.0 1.0 CB A:PHE28 4.8 16.9 1.0 O A:HOH437 4.8 38.8 1.0 CA A:ASP30 4.8 18.3 1.0

Calcium binding site 2 out of 2 in the Hras R97F Crystal Form 1


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 2 of Hras R97F Crystal Form 1 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca205 b:37.1 occ:1.00 O A:HOH360 2.2 43.4 1.0 O A:GLU3 2.3 25.9 1.0 O A:HOH406 2.4 35.6 1.0 OE2 A:GLU76 2.4 39.1 1.0 OE1 A:GLU76 2.7 25.1 1.0 O A:HOH391 2.7 46.0 1.0 O A:HOH427 2.8 48.6 1.0 CD A:GLU76 2.9 34.0 1.0 C A:GLU3 3.5 31.9 1.0 O A:HOH354 4.0 50.2 1.0 CA A:GLU3 4.3 26.9 1.0 CG A:GLU76 4.4 24.0 1.0 N A:GLU3 4.4 25.0 1.0 CB A:GLU3 4.4 29.4 1.0 N A:TYR4 4.4 27.6 1.0 CG A:GLU3 4.5 31.8 1.0 OE2 A:GLU3 4.5 59.3 1.0 CA A:TYR4 4.6 25.7 1.0 N A:LYS5 4.8 21.4 1.0 CG2 A:THR2 5.0 30.7 1.0

C.W.Johnson, S.K.Fetics, K.P.Davis, J.A.Rodrigues, C.Mattos. Allosteric Site Variants Affect Gtp Hydrolysis on Ras. Protein Sci. E4767 2023.
ISSN: ESSN 1469-896X
PubMed: 37615343
DOI: 10.1002/PRO.4767