Calcium in PDB 9ewe: Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex

Enzymatic activity of Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex

All present enzymatic activity of Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex:
2.7.7.7;

Protein crystallography data

The structure of Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex, PDB code: 9ewe was solved by A.Nourisson, A.Haouz, S.Missoury, M.Delarue, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	41.55 / 3.04
*Space group*	P 2₁ 2₁ 2₁
*Cell size a, b, c (Å), α, β, γ (°)*	55.801, 62.251, 138.114, 90, 90, 90
*R / R_free (%)*	24.2 / 27.9

Other elements in 9ewe:

The structure of Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

Calcium Binding Sites:

The binding sites of Calcium atom in the Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex (pdb code 9ewe). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex, PDB code: 9ewe:

Calcium binding site 1 out of 1 in 9ewe

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Calcium Binding Sites List in 9ewe

Calcium binding site 1 out of 1 in the Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex

Mono view

Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Dna Polymerase Lambda I493K, E529D, CA2+ Ground State Ternary Complex within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
A:Ca601 b:35.8 occ:1.00	OG	A:SER417	2.8	38.0	1.0
	O	A:HOH717	2.9	4.5	1.0
	OD1	A:ASP427	3.0	31.1	1.0
	N	A:SER417	3.3	34.2	1.0
	CB	A:SER417	3.5	37.7	1.0
	CA	A:SER417	4.0	36.0	1.0
	N	A:ASP427	4.2	33.0	1.0
	N	A:GLY426	4.2	30.8	1.0
	CG	A:ASP427	4.3	28.5	1.0
	CA	A:GLY416	4.3	37.9	1.0
	C	A:GLY416	4.3	27.9	1.0
	O	A:ASP427	4.4	33.7	1.0
	OD2	A:ASP429	4.4	38.2	1.0
	CA	A:GLY426	4.6	31.4	1.0
	NH2	A:ARG386	4.8	32.0	1.0
	C	A:GLY426	4.9	28.9	1.0
	C	A:ASP427	4.9	26.2	1.0
	CA	A:ASP427	5.0	30.5	1.0

Reference:

A.Nourisson, A.Haouz, S.Missoury, M.Delarue. Dna Polymerase Lambda I493K, E529D, Dctp:at CA2+ Ground State Ternary Complex To Be Published.

Page generated: Mon Aug 4 18:49:17 2025

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