Calcium in PDB 9fbs: Deletion Mutant of Chitinase MMCHI60

All present enzymatic activity of Deletion Mutant of Chitinase MMCHI60:
3.2.1.14;

The structure of Deletion Mutant of Chitinase MMCHI60, PDB code: 9fbs was solved by M.Bejger, W.Rypniewski, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.13 / 2.35
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	50.764, 74.009, 172.538, 90, 90, 90
R / Rfree (%)	18.6 / 26.6

The structure of Deletion Mutant of Chitinase MMCHI60 also contains other interesting chemical elements:

Sodium

(Na)

1 atom

The binding sites of Calcium atom in the Deletion Mutant of Chitinase MMCHI60 (pdb code 9fbs). This binding sites where shown within 5.0 Angstroms radius around Calcium atom.
In total only one binding site of Calcium was determined in the Deletion Mutant of Chitinase MMCHI60, PDB code: 9fbs:

Calcium binding site 1 out of 1 in the Deletion Mutant of Chitinase MMCHI60


Mono view


Stereo pair view

A full contact list of Calcium with other atoms in the Ca binding site number 1 of Deletion Mutant of Chitinase MMCHI60 within 5.0Å range: probe atom residue distance (Å) B Occ A:Ca402 b:77.8 occ:1.00 O A:ALA236 2.7 39.7 1.0 O A:ASN239 2.8 40.1 1.0 NZ A:LYS243 3.2 48.5 1.0 CE A:LYS243 3.5 48.4 1.0 CB A:PHE246 3.5 35.4 1.0 N A:ASN239 3.5 38.1 1.0 C A:GLN237 3.6 35.2 1.0 O A:GLN237 3.6 31.5 1.0 C A:ASN239 3.6 41.1 1.0 CD A:LYS243 3.7 43.0 1.0 CG A:PHE246 3.7 34.8 1.0 CA A:GLN237 3.8 36.4 1.0 C A:ALA236 3.8 36.9 1.0 CA A:LYS243 3.9 44.2 1.0 CD2 A:PHE246 4.0 36.4 1.0 CA A:ASN239 4.0 37.9 1.0 N A:ASN238 4.1 31.8 1.0 N A:GLN237 4.2 37.2 1.0 N A:LYS243 4.3 42.2 1.0 O A:LEU242 4.3 39.0 1.0 CB A:ASN239 4.4 40.7 1.0 C A:LEU242 4.4 41.2 1.0 CD1 A:PHE246 4.4 32.7 1.0 CB A:LYS243 4.4 43.1 1.0 C A:ASN238 4.5 40.9 1.0 CG A:LYS243 4.7 42.8 1.0 CA A:ASN238 4.7 38.3 1.0 N A:ASP240 4.7 45.2 1.0 O A:HOH531 4.8 46.2 1.0 O A:LYS243 4.9 40.7 1.0 C A:LYS243 4.9 44.1 1.0 CE2 A:PHE246 4.9 35.8 1.0 CA A:PHE246 4.9 37.3 1.0

M.Bejger, P.H.Malecki, W.Rypniewski. Probing the Structure and Thermodynamics of A Psychrophilic Chitinase To Be Published.